[Genetic analysis and assisted reproductive guidance for two infertile patients with rare small supernumerary marker chromosomes].

Objective: To carry out cytogenetic and molecular genetic analysis for two infertile patients carrying rare small supernumerary marker chromosomes (sSMC).

Methods: Two infertile patients who received reproductive and genetic counseling at CITIC Xiangya Reproductive and Genetic Hospital on October 31, 2018 and May 10, 2021, respectively were selected as the study subjects. The origin of sSMCs was determined by conventional G banding, fluorescence in situ hybridization (FISH) and copy number variation sequencing (CNV-seq).

Tapered Fiber Bioprobe Based on U-Shaped Fiber Transmission for Immunoassay.

In this paper, a tapered fiber bioprobe based on Mach-Zehnder interference (MZI) is proposed. To retain the highly sensitive straight-tapered fiber MZI sensing structure, we designed a U-shaped transmission fiber structure for the collection of optical sensing signals to achieve a miniature-insert-probe design. The spectrum responses from the conventional straight-tapered fiber MZI sensor and our proposed sensor were compared and analyzed, and experimental results showed that our proposed sensor not only has the same sensing capability as the straight-tapered fiber sensor, but also has the advantages of being flexible, convenient, and less liquid-consuming, which are attributed to the inserted probe design.

Ultra-sensitive all-optical phase modulation based on a fiber optic interferometer combined with TiCT MXene-incorporated PDMS.

This study proposes an all-optical phase modulator based on a compact fiber optic interferometer combined with TiCT MXene-incorporated PDMS. Due to the high photothermal conversion efficiency of MXene and the high thermo-optic coefficient of PDMS, changes in pump power can be effectively converted into refractive index (RI) variations in the PDMS. Then, by employing a fiber optic interferometer with a high RI response, ultra-sensitive all-optical phase modulation can be realized.

Titanium Nitride Modified Fiber Optic Interferometer for Refractive Index Sensitivity Enhancement.

As one of the most well-established biocompatible transition metal nitrides, titanium nitride (TiN) has been widely applied for fiber waveguide coupling device applications. This study proposes a TiN-modified fiber optic interferometer. Benefiting from the unique properties of TiN, including ultrathin nanolayer, high refractive index, and broad-spectrum optical absorption, the refractive index (RI) response of the interferometer is greatly enhanced, which is desired all the time in the field of biosensing.

Biallelic variants in KCTD19 associated with male factor infertility and oligoasthenoteratozoospermia.

Study Question: Can whole-exome sequencing (WES) reveal new genetic factors responsible for male infertility characterized by oligozoospermia?

Summary Answer: We identified biallelic missense variants in the Potassium Channel Tetramerization Domain Containing 19 gene (KCTD19) and confirmed it to be a novel pathogenic gene for male infertility.

What Is Known Already: KCTD19 is a key transcriptional regulator that plays an indispensable role in male fertility by regulating meiotic progression. Kctd19 gene-disrupted male mice exhibit infertility due to meiotic arrest.

Analysis and improvement of dynamic range in a time-division-multiplexing interferometric fiber-optic sensor array.

Dynamic range, the ratio of maximum detectable amplitude, and the noise level, are of great importance in characterizing the performance of an interferometric fiber-optic sensor (IFOS). Here we analyze the dependence of dynamic range on carrier frequency and repetition frequency in a time-division-multiplexing (TDM) IFOS array using a heterodyne detection scheme. A novel, to the best of our knowledge, demodulation method with higher carrier frequency and an upgraded algorithm is proposed to improve the dynamic range.

Femtosecond laser written ultra-weak Fabry-Perot array for distributed absolute temperature profile sensing with high spatial resolution.

In this paper, high spatial-resolution distributed temperature sensing has been realized based on a femtosecond laser written ultra-weak Fabry-Perot Array (FPA). 50 identical Fabry-Perot cavities are fabricated in a 10 mm long optical fiber by femtosecond laser point-by-point written technology, and the corresponding spatial resolution is as high as 200 µm. Besides, by employing the total phase demodulation method, the optical path lengths (OPLs) in the ultra-weak FPA are successively demodulated based on the Rayleigh backscattering signal recorded by an optical frequency domain reflectometry (OFDR), and therefore the absolute temperature values instead of the relative ones can be obtained.

Ultrasensitive deafness gene DNA hybridization detection employing a fiber optic Mach-Zehnder interferometer: Enabled by a black phosphorus nanointerface.

The rapid and efficient detection of deafness gene DNA plays an important role in the clinical diagnosis of deafness diseases. This study demonstrates the ultrasensitive detection of complementary DNA (cDNA) by employing a nanointerface-sensitized fiber optic biosensor. The sensor consists of SMF-TNCF-MMF-SMF (abbreviated as STMS) structure with lateral offset.

Indirect charging of carbon by aqueous redox mediators contributes to the enhanced desalination performance in flow-electrode CDI.

Reversible electrochemical separation based on flow electrodes (e.g., flow-electrode capacitive deionization (FCDI)) is promising to desalinate brackish water, a reliable alternative source of freshwater.

TiCN MXene-based ultra-sensitive optical fiber salinity sensor.

A TiCN MXene enabled ultra-sensitive optical fiber sensor is proposed, and a salinity measurement is conducted to evaluate its sensing performance in a low-concentration target molecule detection environment. Owing to the abundance of hydrophilic functional groups (-O, -F, and -OH), large specific surface, and broad-spectrum absorption characteristics of the MXene layers, the sensing performance of the MXene-incorporated sensor is greatly improved and an ultra-high salinity sensitivity of -5.34 nm/‰ is achieved (equivalent to a refractive index sensitivity of -33429 nm/RIU).

Plasmonic tapered-fiber interference sensor for simultaneously detecting refractive index and temperature.

A single-optic-fiber sensor is proposed to simultaneously detect the refractive index (RI) and temperature (T) at a single wavelength band. This sensor is based on the mixed effects of Mach-Zehnder interference (MZI) and surface plasmon resonance (SPR), where MZI is excited by a tapered-fiber structure, and SPR is stimulated by a 45 nm gold film on the tapered-fiber surface. The detection signal of an SPR spectrum superimposed on interference stripes was obtained.

Multiplexed Weak Waist-Enlarged Fiber Taper Curvature Sensor and Its Rapid Inline Fabrication.

This study proposes a multiplexed weak waist-enlarged fiber taper (WWFT) curvature sensor and its rapid fabrication method. Compared with other types of fiber taper, the proposed WWFT has no difference in appearance with the single mode fiber and has ultralow insertion loss. The fabrication of WWFT also does not need the repeated cleaving and splicing process, and thereby could be rapidly embedded into the inline sensing fiber without splicing point, which greatly enhances the sensor solidity.

In situ potential measurement in a flow-electrode CDI for energy consumption estimation and system optimization.

Flow electrode capacitive deionization (FCDI) is a promising electrochemical technique for brackish water desalination; however, there are challenges in estimating the distribution of resistance and energy consumption inside a FCDI system, which hinders the optimization of the rate-limiting compartment. In this study, energy consumption of each FCDI component (e.g.

High-sensitivity and large-range fiber optic temperature sensor based on PDMS-coated Mach-Zehnder interferometer combined with FBG.

Although numerous efforts have been dedicated towards developing fiber sensors with high performances, challenges still remain in achieving high-quality temperature sensors with high sensitivity, large measurement range and high stability. This study proposes a compact fiber optic temperature sensor based on PDMS-coated Mach-Zehnder interferometer (MZI) combined with FBG, and it can realize both high-sensitivity and large-range temperature measurement. The MZI is based on Thin No-Core Fiber (TNCF) with lateral-offset.

Simultaneous enhancement of treatment performance and energy recovery using pyrite as anodic filling material in constructed wetland coupled with microbial fuel cells.

Constructed wetland coupled with microbial fuel cells (CW-MFCs) are a promising technology for sustainable wastewater treatment. However, the performance of CW-MFCs has long been constrained by the limited size of its anode. In this study, we developed an alternative CW-MFC configuration that uses inexpensive natural conductive pyrite as an anodic filling material (PyAno) to extend the electroactive scope of the anode.

Four-wave mixing-based photonic crystal fiber microfluid sensor with embedded U-shape microslits.

In this paper, we propose a four-wave mixing-based photonic crystal fiber (PCF) microfluid sensor, and two U-shape microslits fabricated by a femtosecond laser are embedded into the sensor for real-time microfluid measurement. Theoretical and experimental results prove that the signal wavelength is sensitive to both the refractive index (RI) and the material dispersion property of the liquid sample filled into the air channels. For different aqueous target samples at low concentrations, the responses of signal wavelength are consistent with each other.

Optimized localization algorithm of dual-Sagnac structure-based fiber optic distributed vibration sensing system.

In this study, an optimized localization algorithm is proposed for a dual-Sagnac structure-based fiber optic distributed vibration sensing (DVS) system. Different from the previous localization algorithms, the spectrum peak ratio of the interference signals in the frequency domain is applied for localization calculation, and the localization accuracy is effectively improved for the interference signal with low Signal-Noise-Ratio (SNR). Besides, the proposed optimized algorithm can solve the difficult problem of multi-point vibration localization by employing a continuous low-coherence light source, which largely reduces the system cost.

Common-mode noise self-suppressed 3-component fiber optic accelerometer based on low-reflectivity Bragg gratings.

Common-mode noises (CMNs) are frequent in the fiber optic accelerometer, and their suppression is extremely important, particularly in the ultra-weak signal detection application, e.g., micro-seismic monitoring.

The genetic cause of intellectual deficiency and/or congenital malformations in two parental reciprocal translocation carriers and implications for assisted reproduction.

Purpose: To elucidate the genetic cause of intellectual deficiency and/or congenital malformations in two parental reciprocal translocation carriers and provide appropriate strategies of assisted reproductive therapy (ART).

Materials And Methods: Two similar couples having a child with global developmental delay/intellectual disability symptoms attended the Reproductive and Genetic Hospital of CITIC-Xiangya (Changsha, China) in 2017 and 2019, respectively, in order to determine the cause(s) of the conditions affecting their child and to seek ART to have a healthy baby. Both of the healthy couples were not of consanguineous marriage, denied exposure to toxicants, and had no adverse life history.

Interrogation technique analyses of a hybrid fiber optic sensor based on SPR and MMI.

This study evaluates the interrogation techniques of a hybrid fiber optic sensor based on surface plasmon resonance (SPR) and multimode interference (MMI). The sensor is based on a single mode, fiber-no core, fiber-single mode fiber (SMF-NCF-SMF) structure with a deposited gold film layer. Both SPR and MMI effects are excited in a single sensor structure without enlarging the device size.

High-performance fiber sensor via Mach-Zehnder interferometer based on immersing exposed-core microstructure fiber in oriented liquid crystals.

Rapid technology development and various applications show great demands for high-quality temperature sensors with super-sensitivity, broad working temperature ranges, excellent linearity and high stability. Although tremendous efforts have been dedicated towards developing fiber sensors with high performance, challenges still remain in achieving all of the four parameters. Herein, we fabricate a fiber sensor via a Mach-Zehnder interferometer (MZI) combined with a liquid crystal (LC)-filled microtube, where the LC in the microtube is uniformly orientated.

Analysis of molecular cytogenetic features and PGT-SR for two infertile patients with small supernumerary marker chromosomes.

Research Question: Can preimplantation genetic testing for structural rearrangement (PGT-SR) with next-generation sequencing (NGS) be used to infertile patients carrying small supernumerary marker chromosomes (sSMCs)?

Design: In this study, two infertile patients carrying ring sSMCs were recruited. Different molecular cytogenetic techniques were performed to identify the features of the two sSMCs, followed by clinical PGT-SR cycles.

Results: The results of G-banding and FISH showed that patient 1's sSMC originated from the 8p23-p10 region, with a resulting karyotype of [ 47,XY, del(8)(p23p10), +r(8)(p23p10).

Distributed fiber optics disturbance sensor using a dual-Sagnac interferometer.

We demonstrate a distributed fiber optics disturbance sensing system based on a dual-Sagnac interferometer that uses one broadband light source and works at the same wavelength. This proposed sensing system is able to reduce the coherent noise and avoid the influence of polarization crosstalk effectively. Experimental results show that, for the presented prototype system with the fiber loop of 1.

High-accuracy transient response fiber optic seismic accelerometer using a shock-absorbing ring as a mechanical antiresonator.

This study proposes a high-accuracy transient response fiber optic seismic accelerometer based on the resonance suppression mechanism. A shock-absorbing ring is embedded in the accelerometer structure, which acts as a mechanical antiresonator. The experimental results show that the sensitivity at the resonance frequency is suppressed by 21.