Investigating Uranium Concentrations in Groundwaters in the State of Idaho Using Kinetic Phosphorescence Analysis and Inductively Coupled Plasma Mass Spectrometry.

The determination of uranium concentrations in natural water samples is of great interest due to the environmental consequences of this radionuclide. In this study, 380 groundwater samples from various locations within the state of Idaho were analyzed using two different techniques. The first method was Kinetic Phosphorescence Analysis (KPA), which gives the total uranium concentrations in water samples.

Motility patterns of filamentous sulfur bacteria, Beggiatoa spp.

The large sulfur bacteria, Beggiatoa spp., live on the oxidation of sulfide with oxygen or nitrate, but avoid high concentrations of both sulfide and oxygen. As gliding filaments, they rely on reversals in the gliding direction to find their preferred environment, the oxygen-sulfide interface.

Filamentous sulfur bacteria, Beggiatoa spp., in arctic marine sediments (Svalbard, 79 degrees N).

Fjord sediments on the west coast of the arctic archipelago Svalbard were surveyed to understand whether large filamentous sulfur bacteria of the genus Beggiatoa thrive at seawater temperatures permanently near freezing. Two sediments had abundant populations of Beggiatoa, while at six sites, only sporadic occurrences were observed. We conclude that Beggiatoa, although previously unnoticed, are widespread in these arctic fjord sediments.

Temperature regulation of gliding motility in filamentous sulfur bacteria, Beggiatoa spp.

The response of gliding motility to changing temperatures was studied in filaments of the large sulfur bacteria Beggiatoa from arctic, temperate and tropical marine environments. The general shape of the gliding speed vs. temperature curves from all three locations was similar, but differed in the maximal gliding speed of the filaments, optimum temperature and the temperature range of motility.

Initial investigation OF 222Rn in the Tbilisi urban environment.

Georgia has geological formations with high uranium content, and several buildings are built with local materials. This can create potentially high radon exposures. Consequently, studies to mitigate these exposures have been started.

Radiological studies in the hot spring region of Mahallat, Central Iran.

Five hot springs called 'Abegarm-e-Mahallat', located in the central part of Iran, have a mean water temperature of 46 +/- 1 degrees C and are used by visitors as spas. This is an area of high natural radiation background due to the presence of (226)Ra and its decay products in the deposited travertine (CaCO(3)). The mean concentration of (226)Ra in these hot springs, measured by the emanation method, ranged from 0.

Process development of a recombinant antibody/interleukin-2 fusion protein expressed in protein-free medium by BHK cells.

The production, purification and stability of quality (in terms of integrity and glycosylation) of an antibody/interleukin-2 fusion protein with potential application in tumour-targeted therapy expressed in BHK21 cells are described. Consistency of the product throughout time was determined by analysis of glycosylation of the fusion protein using MALDI-TOF mass spectroscopy and HPAEC-PAD combined with product integrity studies by SDS-PAGE and Western blotting. These investigations showed consistent expression in terms of integrity and of three major oligosaccharide structures of the fusion protein after 62 generations.

Crystal structure of the extracellular segment of integrin alpha Vbeta3.

Integrins are alphabeta heterodimeric receptors that mediate divalent cation-dependent cell-cell and cell-matrix adhesion through tightly regulated interactions with ligands. We have solved the crystal structure of the extracellular portion of integrin alphaVbeta3 at 3.1 A resolution.

Implementation of the MARSSIM philosophy to evaluate the remedial status of a radioactive waste disposal site at the Idaho National Engineering and Environmental Laboratory. Multi-Agency Radiation Survey and Site Investigation Manual.

Implementation of the MARSSIM remedial-verification protocol at a radioactive waste disposal site is described in some detail to provide a record of the utility of this process. The selected site was the Stationary Low-Power Reactor No. 1 burial ground at the Idaho National Engineering and Environmental Laboratory.

Interspecies comparison of in vitro plasma degradation of dynorphin A 1-13.

Enzymatic cleavage of peptides might release metabolites with distinct pharmacological profile. Interspecies differences in the metabolism of peptides might represent a potential source of error when animal data are intended to predict the human situation. Therefore, the metabolism of dynorphin (Dyn) A1-13 was investigated in the plasma of various species used in experimental practice (monkey, rabbit, rat, guinea pig) and compared to previously reported human data.

Interhemispheric approach with callosal resection for distal anterior cerebral artery aneurysms. Technical note.

Distal anterior cerebral artery aneurysms are commonly found near the genu of the corpus callosum. While these aneurysms may be surgically obliterated through a variety of approaches, exposure via the interhemispheric fissure is used by many surgeons. Early identification of the afferent artery may be difficult with this approach, however, particularly if the aneurysm lies just beneath the genu of the corpus callosum.

Cerebral vasoconstriction as a complication of carotid endarterectomy. Case report.

The case is presented of an elderly man with an acute confusional state occurring soon after he had undergone a right carotid endarterectomy. Angiography demonstrated segmental areas of cerebral vasoconstriction and an electroencephalogram revealed periodic lateralized epileptiform discharges, both involving the right hemisphere. Cerebral hyperperfusion has been implicated in the genesis of several transient neurological syndromes following carotid endarterectomy.

Traumatic atlanto-occipital dislocation. Case report.

Traumatic atlanto-occipital dislocation is a serious injury that is usually fatal. The number of patients surviving this injury, however, appears to be increasing, and most of these survivors are children. This may reflect an improvement in emergency transport services.

Polycationic polypeptides: a possible model for the penetration-enhancing factor in the invasion of host cells by Toxoplasma gondii.

The effect of polycationic polypeptides (polylysine, polyarginine and polyhistidine) on the invasion of mammalian cells and plant protoplasts by Toxoplasma gondii was studied. In JM cells, a human lymphoblastoid cell line with T cell characteristics, all polycationic polypeptides used increased the invasion rate in a concentration-dependent manner. This effect and the morphological changes revealed by electron microscopy resembled the action of the penetration-enhancing factor previously described by E.

Reversible inhibition of poxvirus replication by cycloheximide during the early phase of infection.

Infection of primary chick embryo fibroblasts with Vaccinia WR, IHD-W, and cowpox virus even at high m.o.i.

Immunohistochemical identification of T-lymphocytes in the central nervous system of patients with multiple sclerosis and subacute sclerosing panencephalitis.

T-lymphocytes were identified in frozen brain sections derived from patients with chronic inflammatory disorders of the CNS by using a specific heteroantiserum and the unlabelled antibody enzyme method. Clusters of T-cells were found in post-mortem material of cases with multiple sclerosis (MS) and subacute sclerosing panencephalitis (SSPE). The results suggest that T-lymphocytes are involved in the pathogenesis of both MS and SSPE.

Tubular subviral structure produced in adenovirus-infected KB cells.

At 40 to 90 h after infection with high multiplicities of adenovirus type 2, 4 to 15% of KB cells produced relatively few intranuclear virions detectable by electron microscopy. The nuclei of these cells were found to contain long tubular structures which were made up at least in part by adenovirus structural proteins. The ends of these tubular structures were frequently terminated by morphologically normal adenovirions in varying degrees of completeness.

DNA of Bacillus subtilis bacteriophage SPP1: physical mapping and localization of the origin of replication.

The genome of Bacillus subtilis bacteriophage SPP1, a linear, 28.5-megadalton DNA duplex, was mapped by analysis with the restriction endonucleases endo R.Sal I, Sma I, Xba I, Bgl I, Bgl II, and EcoRI.

Kinetics of horseradish peroxidase migration through cerebral cortex.

This study provides a semiquantitative description of the migration of the extracellular space marker, horseradish peroxidase (HRP), through the cerebral cortex. Following a continuous subarachnoid infusion of HRP, this marker was fixed rapidly within the cortical extracellular space (ECS) by intravascular aldehyde perfusion fixation. Microscopic measurements of the maximum depth of penetration of HRP into the cortex perpendicular to the pial surface were taken from coronal whole brain sections of rabbits that had been exposed to HRP for varying periods of time.

Surgical anatomy of the proximal anterior cerebral artery.

The authors present this study of proximal anterior cerebral arteries in the normal human to provide a clearer basis for strategy in aneurysm surgery. They describe patterns of origin of branches, their subarachnoid course, and parenchymal distribution. Branches that originate from the anterior cerebral artery at the internal carotid bifurcation perfuse the genu and contiguous posterior limb of the internal capsule and the rostral thalamus.