Biodiesel Production from Soybean Oil Using a Free-Enzyme and Whole-Cell Dual Lipase System as a Biocatalyst.

A dual lipase system has been developed to convert soybean oil into biodiesel through synergistic catalysis of Thermomyces lanuginosus lipase (TLL) and Yarrowia lipolytica lipase 2 (YLL) in this study. Pichia pastoris recombinant strains expressing lipases were successfully constructed, and the activities of TLL and YLL in the fermentation supernatant reached 23,142.71 ± 280.

Biodiesel Production from Waste Oil Through Efficient Enzymatic Synthesis Using Yarrowia lipolytica Lipase 2 in the Presence of Glucose, β-Cyclodextrin, or G50.

In this study, the liquid lipase Yarrowia lipolytica lipase 2 (YLLip2) expressed by Pichia pastoris GS115 was used to produce biodiesel from waste oil. Four signal peptides were compared to express YLLip2 in P. pastoris, among which SP23 exhibited greater secretion performance.

Efficient Expression of Candida antarctica Lipase B in Pichia pastoris and Its Application in Biodiesel Production.

Lipase B from Candida antarctica (CALB) is an important biocatalyst with many potential applications. However, original CALB is usually with lower enzyme activity and also costly to produce from Candida antarctica; hence, it is often necessary to prepare recombinant CALB through gene heterologous expression. In this research, seven promoters and five signal peptides were compared respectively for expressing codon-optimized CALB in Pichia pastoris, and then recombinant P.