Subspecies Typing of Based on Ribosomal Subunit Protein Mass Variation by MALDI-TOF MS.

A ribosomal subunit protein (rsp)-based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) method was developed for fast subspecies-level typing of (Group B , GBS), a major cause of neonatal sepsis and meningitis. A total of 796 GBS whole genome sequences, covering the genetic diversity of the global GBS population, were used to predict molecular mass variability of 28 rsp and to identify unique rsp mass combinations, termed "rsp-profiles". The established GBS typing scheme was validated by MALDI-TOF MS analysis of GBS isolates at two independent research sites in Europe and South East Asia.

Maternal vaginal colonization with selected potential pathogens of neonatal sepsis in the era of antimicrobial resistance, a single center experience from Sri Lanka.

Background: Maternal vaginal colonization with antibiotic resistant organisms is a growing concern in countries with high antibiotic resistance rates.

Methods: A low vaginal swab was collected from mothers on admission, on discharge and a peri-rectal swab was collected from the neonates born to these mothers on discharge. Routine microbiological methods were used to identify the colonization rates for Escherichia coli, Klebsiella spp.

Spread of resistant gram negatives in a Sri Lankan intensive care unit.

Background: Infections with multi drug resistant (MDR) organisms are a major problem in intensive care units (ICUs). Proper infection control procedures are mandatory to combat the spread of resistant organisms within ICUs. Well stablished surveillance programmes will enhance the adherence of the staff to infection control protocols.