An inventory of human light exposure behaviour.

Light exposure is an essential driver of health and well-being, and individual behaviours during rest and activity modulate physiologically relevant aspects of light exposure. Further understanding the behaviours that influence individual photic exposure patterns may provide insight into the volitional contributions to the physiological effects of light and guide behavioural points of intervention. Here, we present a novel, self-reported and psychometrically validated inventory to capture light exposure-related behaviour, the Light Exposure Behaviour Assessment (LEBA).

The importance of including position and viewing direction when measuring and assessing the lighting conditions of office workers.

Background: Light and alertness studies have applied different measurement methodologies to determine lighting conditions. However, it has been demonstrated that researchers rarely measure or describe the lighting conditions of their studies in sufficient detail to generalize conclusions or derive universal guidelines.

Objective: Part I of this paper summarizes the current measurement methodologies used in light and alertness studies to potentially identify methodological issues.

Recommendations for measuring non-image-forming effects of light: A practical method to apply on cognitive impaired and unaffected participants.

Background: The non-image-forming effects of luminous radiation on people with intellectual disabilities or dementia received attention from researchers. Such studies, however, have generally been conducted using disparate methodologies which precludes generalization and reproducibility.

Objective: The aim of this study was to determine the practical applicability of measurement devices for studies investigating non-image-forming effects of luminous radiation, specifically for people with intellectual disabilities or dementia.

Cutinase binding and activity at the triolein-water interface monitored by oil drop tensiometry.

Changes of the oil-water interfacial tension resulting from binding of Fusarium solani pisi cutinase and subsequent lipid hydrolysis were investigated using the oil drop technique. An ELISA was developed to determine the amount of cutinase bound to the triolein-water interface after biotinylation of the enzyme. Cutinase irreversibly adsorbs to a maximum value of about 2 mg/m2.

MON-150, a versatile monoclonal antibody against involucrin: characterization and applications.

A monoclonal antibody, designated MON-150, was found serendipitously to react strongly with the granular layer of normal human epidermis and with the upper spinous layers of psoriatic epidermis. From analysis by flow cytometry of cultured human keratinocytes, it appeared that the percentage of MON-150-positive cells strongly increased when the cells reached confluence and the growth fraction declined. To identify the antigen recognized by MON-150, a lysate of human keratinocytes was subjected to affinity chromatography using a MON-150 Sepharose column.

A new photometric method for the quantitation of Fc receptors for murine IgG1 on human monocytes and cell lines.

We have previously shown a polymorphism of human Fc receptors for mouse IgG1 using an EA rosette technique in which human erythrocytes sensitized with a murine IgG1 monoclonal antibody against glycophorin A acted as indicator cells. We now describe a method to quantitate this EA rosetting using the pseudoperoxidase activity present in erythrocytes. This photometric assay allows the sensitive quantitative determination of Fc receptor expression on human monocytes and cell lines.