miR-410-3p is induced by vemurafenib via ER stress and contributes to resistance to BRAF inhibitor in melanoma.

Despite significant development of melanoma therapies, death rates remain high. MicroRNAs, controlling posttranscriptionally gene expression, play role in development of resistance to BRAF inhibitors. The aim of the study was to assess the role of miR-410-3p in response to vemurafenib-BRAF inhibitor.

[Ovarian tumors in pregnancy].

Objective: The clinical characteristics of pregnancy complicated by ovarian tumors were investigated.

Material And Methods: A review was performed of patients who were seen with ovarian tumors in pregnancy from January 1985 to August 2000. We included patients with simple or complex tumors > 5 cm that were seen on ultrasonographic evaluation.

[Analysis of clinical usefulness of the cytologic smear results for pregnant women using the Bethesda system].

The aim of this paper was to analyse the clinical usefulness of cytological smear results for pregnant women by use of Bethesda system. The examinations were carried for 5 years and correct results of cytological assays were achieved from 3265 patients. Low and high grade squamous intraepithelial cells were reported in 4.

New renin inhibitors with pseudodipeptidic units in P(1)-P(1') and P(2')-P(3') positions.

A series of four new potential renin inhibitors has been synthesized. The structure of the compounds was designed in such a way as to produce agents resistant to enzymatic degradation, metabolically stable, possibly potent and with improved oral absorption. All positions of the 8-13 fragment of the human angiotensinogen were occupied by unnatural units (two unnatural amino acids in positions P(3) and P(2) and two pseudodipeptides in positions P(1)-P(1') and P(2')-P(3')).

Function of insulin-like growth factor (IGF-I) and its binding protein (IGFBP-1) in pathological proliferation of endometrium.

The purpose of this project was the investigation of insulin, IGF-I and IGFBP-1 concentrations in the serum of patients with pathological changes in endometrium. IGF-I and its binding protein (IGFBP-1) stimulate cell growth and differentiation of the normal and neoplastic endometrium. The mean values of IGF-I concentrations in the group of patients with atypical hyperplasia and endometrial carcinoma were significantly higher than in the control group with normal endometrium.

[Detection of human papillomavirus genome in cervical squamous intraepithelial lesions].

Objectives: The aim of this work was the assessment of the correlation between different types of HPV and abnormal stages of uterine cervix.

Materials And Methods: The smears were simultaneously collected for both cytooncologic examination and PCR identification of HPV viruses. Cytologic smears were classified according to the Bethesda system.

[Diagnostic procedures of pathological bleeding in women population: comparison of hysteroscopy, ultrasonography and microscopic examination endometrium].

The goal of this study was to asses the diagnostic value of hysteroscopy, cytology, ultrasonography and histopathology in various pathological states in endometrium. 250 patients with abnormal uterine bleeding were examined. The wide range of several diagnoses were achieved with 8 cases of cancer.

[Chromosome instability in women with genital organs carcinoma].

Objectives: The aim of the current work was the assessment of the mutagen susceptibility of chromosomes of patients with carcinomas in comparison to healthy volunteers. It was interesting whether the bleomycin assay can be useful for searching for more susceptible to cancer disease individuals.

Materials And Methods: There were 4 groups of patients analysed: controls and three test groups (patients with uterine cervix carcinoma, endometrium carcinoma and ovarian carcinoma).

[The influence of demographic and behavioral factors on the risk of human papillomavirus infection].

Objectives: The aim of this work was the assessment of correlation between different types of HPV and chosen factors: the number of pregnancies, childbirths and smoking habits.

Materials And Methods: Patients were examined in the Gynaecology Clinic, Silesian Medical Academy in Zabrze from 1998 until 2000. They were either hospital patients or women subjected to routine cytological examination.

Changes in insulin-like growth factor I, 17-b-estradiol, and progesterone in postmenopausal women with benign and malignant ovarian tumours.

Background: The aim of this research was to observe changes in IGF-I and steroid hormones in postmenopausal women with benign and malignant ovarian tumors.

Material And Methods: The concentrations of insulin-like growth factor I (IGF-I), 17-b-estradiol and progesterone were examined by radioimmunological assay in three groups of postmenopausal women: patients with malignant ovarian tumors, patients with benign ovarian tumors, and healthy women. In all three groups the age and body mass index (BMI) were comparable.

[Laparotomy performed in pregnant women due to non-obstetrical causes].

Objective: The clinical characteristics of laparotomy during pregnancy were investigated.

Materials And Methods: A series of 24 cases of laparotomy during pregnancy was investigated with respect to clinicopathologic features and outcome.

Results: Among 23 cases there were 20 ovarian tumors, 2 acute appendicitis and 2 uterine fibroid.

[Comparison of conservative and laparoscopic treatment of PCOS].

Objective: Aim of study contained clinical analysis of results conservative (Clostilbegyt) and laparoscopic (electro-catheterisation of ovaries) methods of treatment PCOS. Authors analysed count of pregnancy, deliveries and serum concentrations of LH, FSH, LH/FSH ratio, 17 beta E2 before and after treatment.

Material And Methods: Stady included 50 obese patients (group A, BMI > 28) treated by Clostilbegyt and 50 lean patients (group B, BMI < 28) treated by laparoscopic ovarian electrocautery.

[The method of treatment of functional evident hyperprolactinemia in sterile women].

Objectives And Design: The aim of study was evaluation of three methods of treatment of functional, evident hyperprolactinemia in women with primary or secondary sterility by use of Bromoergocriptine, Dopergin or Norprolac.

Materials And Methods: Our study contained 65 patients divided into three groups treated by use three methods. We used RIA-method for evaluation serum concentrations of h-PRL.

[Carrier of numerical chromosome abnormalities in infertile married couples].

Objectives: Genetic anomalies are one of many conditions causing infertility.

Design: The aim of the study was to define the frequency of numerical chromosome aberrations in infertile married couples.

Materials And Methods: Metaphasal chromosomes were analysed by G-T-G stration in 650 patients, i.

[Urinary tract infections in pregnancy: perinatal problem].

The authors presented the frequency of urinary tract infection (u.t.i) in pregnancy in own practice.

[Preliminary assessment of transient evoked acoustic emissions (TEOAE) in newborns depending on selected parameters of delivery complications with particular analysis of newborns with hypotrophy].

Objectives: Transient evoked otoacoustic emission (TEOAE) is accepted as a good and universal test for screening of the cochlea function in newborns. This method is particularly useful in the newborn group with risk-factors of hearing losses. Early identification of the cochlear pathology gives possibility of supplying with hearing aid instrument and rehabilitation.

[The evaluation of susceptibility to oxidation of selected lipoproteins of pregnant women with type B diabetes mellitus in 2nd and 3rd trimester of pregnancy].

Objectives And Design: The aim of study was estimation of susceptibility on the process of oxidation of selected lipoproteins of pregnant women with IDDM in II, III trimester of pregnancy.

Materials And Methods: Study included 80 women. Patients were divided into four groups: pregnant with IDDM, pregnant healthy, nonpregnant with and without IDDM.

[The occurrence of malformations in newborns from highly polluted environment: own materials from years 1993-1998].

Objectives: Epidemiological rates and the structure of malformations in live born infants from the II Chair and Clinic of Obstetrics and Gynecology in Zabrze in the year 1993-1998 are shown in this paper.

Material And Methods: 5898 newborns with weight over 500 g were examined. 341 histories of development of newborns with malformations were analysed.

New renin inhibitors with hydrophilic C-terminus.

Four new peptide-based renin inhibitors, Boc-Phe(4-OMe)-MePhe-AHPPA-epsilon Ahx-EA (11), Boc-Phe(4-OMe)-MeLeu-AHP-PA-epsilon Ahx-EA (15), Boc-Phe(4-OMe)-MePhe-Sta-epsilon Ahx-EA (20) and Boc-Phe(4-OMe)-MeLeu-Sta-epsilon Ahx-EA (21) have been synthesized in search of structures with improved biological properties. They were designed as compounds with moderate hydrophobicity (5.28, 4.

[Computer analysis of a cardiotocogram and parameters of acid-base balance in capillary blood of the infant].

Our objective in this study was to compare the parameters of the acid-base balance in the capillary blood 60 minutes after delivery with the antenatal cardiotocographic computer analysis. Our study included 64 infants delivered vaginally on term. The basic criterion to classify into the examined group and control group was a pH result of the capillary blood equal to 7.

Renin inhibitors containing nicotinic or isonicotinic acid at the N-terminus. Part V.

Four new compounds: Nic-Phe/4-OMe/-MePhe-Sta-epsilonAhx-OMe/23/,Nic-Phe/4-OMe/-MePhe- Sta-epsilonAhx-Iaa/24/,iNic-Phe/4-OMe/-MeLeu-Sta-ep silonAhx-OMe/29/ and iNic-Phe/4-OMe/-MeLeu-Sta-epsilonAhx-Iaa/30/ have been synthesized in search after renin inhibitors of improved biological properties. Their stability against chymotrypsin activity, solubility in water at pH 7.4, 6.

The effect of intraamniotically administered L-thyroxin on pulmonary efficacy of newborns delivered prematurely.

The aim of the study was to investigate the effect of L-thyroxine on prenatal lung maturation. Forty pregnant women with indications to preterm delivery were divided into two subgroups: A--30 patients suffering from insulin dependent diabetes mellitus, B--10 patients with other pathology of pregnancy. 500 micrograms L-thyroxine "Henning" was injected intraamniotically.

Enzymatically stable renin inhibitors containing statine and 6 aminohexanoic acid. Part IV.

Eight new peptide renin inhibitors: Boc-Phe/4-OMe/His-Sta-epsilonAhx-Iaa(13), Boc-Phe/4-OMe/-His-Sta-episilonAhx-OMe,(21),Boc-Phe/4-OMe/-MePhe-S ta-epsilonAhx-Iaa(27),Boc-Phe/4-OMe/-MePhe-Sta-epsilonAhx-++ +epsilonAhx-Iaa(32),Boc-Phe/4-OMe/-MePhe-Sta-Val-epsilonAhx- OMe (38),Boc-Phe/4-OMe/-MeVal-Sta-Val-Iaa(48),Boc-Phe/4-OMe/-Me Val-Sta-Iaa(51), Boc-Phe/4-OMe/-MeLeu-Sta-epsilonAhx-Iaa (57) have been synthesized in search after compounds of improved biological properties. All peptides were obtained by carbodimide method in solution by stepwise elongation of the peptide chain or by fragment condensation. Their potency was assayed in vitro by a spectrofluorometric method/assay of Leu-Val-Tyr-Ser released from N-acetyltetradecapeptide substrate by renin in the presence of an inhibitor/.

Evaluation of amniotic fluid elastolytic activity: can it be a method of fetal lung maturity assessment? A comparison with Gluck's L/S test.

Amniotic fluid elastolytic activity was assessed in a group of 120 women who delivered preterm infants and in 35 women who delivered at term. Amniotic fluid elastolytic activity decreases as pregnancy progresses. The lecithin-to-sphingomyelin (L/S) ratio in women's amniotic fluid was determined by the method developed by Gluck and associates [6] and elastolytic activity by that developed by Mehdi and associates.

Renin inhibitors containing statine and 6-aminohexanoic acid. Part III.

Five peptide renin inhibitors containing the sequence: Phe-His-Sta-epsilon Ahx (Sta = 4(S)-amino-3(S)-hydroxy-6-methylheptanoic acid, epsilon Ahx = 6-aminohexanoic acid) were synthesized and their potency was assayed in vitro by a spectrofluorometric method (assay of Leu-Val-Tyr-Ser released from N-acetyltetradecapeptide substrate by renin in the presence of an inhibitor). Their stability was tested by assay of Phe and Pro-Phe released after incubation with chymotrypsin. The most potent inhibitor was Boc-Phe-His-Sta-epsilon Ahx-OMe (IC50 = 5 x 10(-9) M/l), the most stable--Boc-Pro-Phe-His-Sta-epsilon Ahx-OMe (resistant to incubation with chymotrypsin for 4 h).