Involvement of human beta-defensin-2 in intracellular signaling: in vitro study.

Aim: To analyze involvement of human beta-defensin-2 (hBD-2) in intracellular signaling in vitro.

Materials And Methods: A431cells were cultured in the presence of 1 microg/ml of recombinant hBD-2 and/or 10 ng/ml EGF. For evaluation of expression of mRNAs for p70S6 kinase, isoforms alpha and beta, RT-PCR analysis was applied.

Immunohistochemical analysis of S6K1 and S6K2 expression in endometrial adenocarcinomas.

Aim: The aim of this study was to analyze the levels 70 kDa ribosomal protein S6 kinase 1 (S6K1) and 70 kDa ribosomal protein S6 kinase 2 (S6K2) expression and S6 ribosomal protein phosphorylation in endometrial adenocarcinomas.

Methods: S6K1/2 expression and phosphorylated ribosomal S6 protein (phS6) content have been detected in formalin fixed, paraffin embedded sections of 50 human endometrial adenocarcinomas with different grade of differentiation and in 13 normal endometrial tissues using immunohistochemical approach with following semiquantitative analysis.

Results: In normal endometrial epithelial cells both S6K1 and S6K2 were expressed on the low level.

[Modifying effect of low-intensive electromagnetic radiation on the irradiated cells].

The normalising effect of low-intensive electromagnetic radiation on the viability of cell culture of L929 line, irradiated with gamma-quantums of 137Cs in a wide dose range was demonstrated.

[The cytotoxic effect of heavy metals on an L-cell culture].

The dependence of some parameters of L-cells culture viability on different concentrations of heavy metals was studied. Considerable cytotoxic effect of low concentrations of nickel (0.025 mcg/ml) and lead (0.