[Activation of specific membrane mechanisms in the myocardium cells on early stages of ischemia].

Electron probe microanalysis was employed to determine the elemental concentration (K,Na,Cl) in a myocyte on cryosections of the papillary muscle of the isolated rat (Wistar) heart. Protocols of global ischemia and ischemic conditions under glucose-free anoxic perfusion were applied. It was shown that global ischemia induces potassium deficiency (94 +/- 2 mM) in the myocyte and an increase in the level of sodium (72 +/- 4 mM) and chlorine (42 +/- 1 mM) in the cytoplasm compared with intact cell (122 +/- 2; 36 +/- 1; 24 +/- 1 mM).

[Analysis of elemental composition (Na/K/Ca) of muscle cells in ischemia in a model of the perfused heart].

It has been hypothesized that cardiac ischaemia induces some changes in the cardiac myocyte element. In the present study we analysed whether the imbalance of potassium, sodium and calcium in cardiomyocyte may be coupled with ischaemic conditions, using a perfused heard as a model. Electron Probe Microanalysis (EPMA) of papillary muscle cryosection was employed to examine the intracellular content of elements.

[A comparative analysis of the intracellular potassium content for the cardiomyocyte in papillary muscle tissue and in a primary heart culture].

This work was aimed to examine potassium contents in heart myocytes. The intracellular concentration of this element was measured with electron probe microanalysis (EPMA). The cardiomyocyte was studied in samples of the papillary muscle tissue and in heart primary culture.