Development of Electrochemical Aptasensor for Lung Cancer Diagnostics in Human Blood.

We describe the preparation and characterization of an aptamer-based electrochemical sensor to lung cancer tumor markers in human blood. The highly reproducible aptamer sensing layer with a high density (up to 70% coverage) on the gold electrode was made. Electrochemical methods and confocal laser scanning microscopy were used to study the stability of the aptamer layer structure and binding ability.

[Ecologo-hygienic criteria and monitoring indices of oil-contaminated peaty soils].

Intensive oil extraction in Khanty-Mansi Autonomous Okrug is not uncommon accompanied by emergency situations giving rise to the pollution of soil, surface and groundwater and causing an elevation in the population morbidity rate. The purpose of the study is to substantiate the basis for information value of hygienic indices of peat soils with low levels of oil contamination: from background values to exceeding the latters as much as 10-20 times, to apply in the study results in hygienic monitoring and oil regulation. The study was performed in accordance with the current methodological regulations.

[Some aspects of avian flu in the Tyumen region].

The list of the actions spent in the summer of 2005 in territory of the Tyumen region, in connection with a destruction of wild birds and poultry from the avian flu is submitted. Except for the quarantine and other sanitary-epidemic measures warning drift of a avian influenza virus in facilities, it is carried out of serological monitoring among workers of poultry-farming facilities and the population of the struck settlements on presence of antibodies to an influenza A (H5N1) virus. Taking into account ways of migration of a wild waterfowl in this territory, probability of contact to it and risk of infection of people, the conclusion about necessity of continuation the monitoring behind circulation of an avian influenza virus in region is made.

[Experimental studies on possible correction of MPEL of ethylenediamine in the workplace air].

The present MAC for ethylenediamine (2 mg/cu m) does not match up-to-date requirements of industrial medicine and requires experimental analysis. Using contemporary methodic approaches, the authors studied toxicodynamics of ethylenediamine, revealed parameters of its toxicity and jeopardy. Biologic effects of ethylenediamine are irritation, allergic signs and changes in central nervous system.

[Deamination reaction of biogenic amines and other nitrogen compounds during the long-term influence of ethylenediamine in an experiment on rats].

Activity and substrate specificity of monoamine oxidase (MAO) were studied in tissues of rats inhaling low concentrations of ethylene diamine for a long time. While the activity of MAO-A (serotonin as a substrate) was decreased in brain, functions of the enzyme were altered: the enzyme acquired the ability to deaminate glucosamine, which is not a substrate of MAO under normal conditions. Ethylene diamine-dependent modification of MAO was responsible for alterations of the enzyme catalytic properties involved in oxidation of substances with specific biochemical functions.