[Effects of astragaloside IV on the expressions of transforming growth factor-β1 and thymic stromal lymphopoietin in a murine model of asthma].

Objective: To observe the effects of astragaloside IV on the airway remodeling and the expressions of transforming growth factor (TGF)-β1 and thymic stromal lymphopoietin (TSLP) in a murine model of asthma.

Methods: Forty-eight BALB/c mice were randomly divided into 4 groups, i.e.

[Alternate-day oral tadalafil for erectile dysfunction with no successful intercourse at the baseline].

Objective: To summarize the experience in the treatment of erectile dysfunction (ED) with no successful intercourse at the baseline with tadalafil.

Methods: We retrospectively analyzed 21 cases of ED with no successful intercourse at the baseline treated with tadalafil on alternate days combined with sex guidance.

Results: After 4 weeks of tadalafil treatment, 19 of the patients achieved successful sexual intercourse, and the IIEF-5 score was remarkably improved as compared with pre-medication (3.

Diffuse interface model of multicomponent vesicle adhesion and fusion.

Prefusion and postfusion states of the biological fusion process between lipid bilayer vesicle membranes are studied in this paper. Based on the Helfrich-type continuum theory, a diffuse interface model is developed which describes the phase changes on the deformable vesicles via a scalar phase field function, and incorporates the adhesion effect between the different phases of the vesicles through a nonlocal interaction potential. Various equilibrium configurations in the prefusion and postfusion states are examined.

Expression of cell-cycle regulatory proteins BUBR1, MAD2, Aurora A, cyclin A and cyclin E in invasive ductal breast carcinomas.

Cyclin A, cyclin E, BUBR1, MAD2 and Aurora A are all cell-cycle regulatory proteins and have been proven to play crucial roles in carcinogenesis. However, their expression patterns in invasive ductal breast carcinoma (IDBC) are controversial and unclear. In this study, we examined the expression status of these candidate proteins in a set of 117 invasive ductal carcinomas, and evaluated their associations with known clinicopathological parameters and the expressions of estrogen receptor, progesterone receptor, Ki-67 and Her-2.

Grape seed proanthocyanidin extract attenuates airway inflammation and hyperresponsiveness in a murine model of asthma by downregulating inducible nitric oxide synthase.

Allergic asthma is characterized by hyperresponsiveness and inflammation of the airway with increased expression of inducible nitric oxide synthase (iNOS) and overproduction of nitric oxide (NO). Grape seed proanthocyanidin extract (GSPE) has been proved to have antioxidant, antitumor, anti-inflammatory, and other pharmacological effects. The purpose of this study was to examine the role of GSPE on airway inflammation and hyperresponsiveness in a mouse model of allergic asthma.

Effect of immunohistochemistry on molecular analysis of tissue samples: implications for microdissection technologies.

Laser-based tissue microdissection is an important tool for the molecular evaluation of histological sections. The technology has continued to advance since its initial commercialization in the 1990s, with improvements in many aspects of the process. More recent developments are tailored toward an automated, operator-independent mode that relies on antibodies as targeting probes, such as immuno-laser capture microdissection or expression microdissection (xMD).

[Intracytoplasmic sperm injection for obstructive azoospermia].

Objective: To sum up the experience in the treatment of obstructive azoospermia by intracytoplasmic sperm injection (ICSI).

Methods: We retrospectively analyzed 107 cases of obstructive azoospermia treated by ICSI in our center from Jan. 2006 to Dec.

Paeonol attenuates airway inflammation and hyperresponsiveness in a murine model of ovalbumin-induced asthma.

Paeonol, the main active component isolated from Moutan Cortex, possesses extensive pharmacological activities such as anti-inflammatory, anti-allergic, and immunoregulatory effects. In the present study, we examined the effects of paeonol on airway inflammation and hyperresponsiveness in a mouse model of allergic asthma. BALB/c mice sensitized and challenged with ovalbumin were administered paeonol intragastrically at a dose of 100 mg/kg daily.

[Over expression of hyperplasia suppressor gene inhibits the malignant phenotype of breast cancer cell].

Objective: To investigate the effect of over expression of human hyperplasia suppressor gene (HSG) on proliferation, invasion, apoptosis and cell cycle of human breast cancer cells and to determine the relationship between HSG and Ras-dependent signaling pathway.

Methods: Full length HSG coding sequences were cloned into plasmid pcDNA3.0.

Adhesion of multicomponent vesicle membranes.

In this work, we study the adhesion of multicomponent vesicle membrane to both flat and curved substrates, based on the conventional Helfrich bending energy for multicomponent vesicles and adhesion potentials of different forms. A phase field formulation is used to describe the different components of the vesicle. For the axisymmetric case, a number of representative equilibrium vesicle shapes are computed and a few energy diagrams are presented which reveal the dependence of the calculated shapes and solution branches on various parameters including both bending moduli and spontaneous curvatures as well as the adhesion potential constants.

Application of Population Dynamics to Study Heterotypic Cell Aggregations in the Near-Wall Region of a Shear Flow.

Our research focused on the polymorphonuclear neutrophils (PMNs) tethering to the vascular endothelial cells (EC) and the subsequent melanoma cell emboli formation in a shear flow, an important process of tumor cell extravasation from the circulation during metastasis. We applied population balance model based on Smoluchowski coagulation equation to study the heterotypic aggregation between PMNs and melanoma cells in the near-wall region of an in vitro parallel-plate flow chamber, which simulates in vivo cell-substrate adhesion from the vasculatures by combining mathematical modeling and numerical simulations with experimental observations. To the best of our knowledge, a multiscale near-wall aggregation model was developed, for the first time, which incorporated the effects of both cell deformation and general ratios of heterotypic cells on the cell aggregation process.

Note: Single-shot continuously time-resolved MeV ultrafast electron diffraction.

We have demonstrated single-shot continuously time-resolved MeV ultrafast electron diffraction using a static single crystal gold sample. An MeV high density electron pulse was used to probe the sample and then streaked by an rf deflecting cavity. The single-shot, high quality, streaked diffraction pattern allowed structural information within several picoseconds to be continuously temporally resolved with an approximately 200 fs resolution.

Extracellular ATP enhances in vitro invasion of prostate cancer cells by activating Rho GTPase and upregulating MMPs expression.

We previously found that in addition to anti-proliferation function, extracellular ATP had a pro-invasion effect on prostate cancer cells, and probably serves as an important regulator of invasion in local microenvironment. However, the underlying mechanism remains unclear. In this study, we demonstrated that ATP increased the motility of prostate cancer cells, and promoted formation of lamellipodia and filopodia.

Apigenin inhibits allergen-induced airway inflammation and switches immune response in a murine model of asthma.

Many flavonoids were demonstrated to possess the antiallergic effect. Here we detected whether apigenin, a flavonoid, can attenuate allergen-induced airway inflammation and what is the possible mechanism in a murine model of asthma. Apigenin decreased the degree of the inflammatory cell infiltration, airway hyperresponsiveness, and total immunoglobulin E levels compared with the ovalbumin group.

Cross-Regulation Between Wnt and NF-κB Signaling Pathways.

Cross-regulation between the Wnt and nuclear factor (NF)-κB signaling pathways has emerged as an important area for the regulation of a diverse array of genes and pathways active in chronic inflammation, immunity, development, and tumorigenesis. The ligands, kinases, transcription factors, and products of their target gene expression are involved in cross-regulation of these two signaling pathways. Both β-catenin and NF-κB activate inducible nitric oxide synthase (iNOS) gene expression; however, β-catenin also exerts an inhibitory effect on NF-κB-mediated transcriptional activation, including iNOS.

[Reliability and validity of a Chinese version of the Quality of Life in Epilepsy Inventory (QOLIE-31-P)].

Objective: To develop and validate a Chinese version of the Patient-Weighted Quality of Life in Epilepsy Questionnaire (QOLIE-31-P).

Methods: The original English version of the QOLIE-31-P was translated into the Chinese language. The inventory was then completed by 200 adult patients with epilepsy; and 49 patients also completed the scale twice within three weeks.

[A new method for REZ-1 cochlear implant electrode array insertion].

Objective: To develop a new method for REZ-1 cochlear implant electrode array insertion.

Methods: REZ-1 cochlear implant electrode array was implanted in 22 human temporal bone specimens. Cochlear diameters were measured from spiral CT scan before implantation.

Naringenin inhibits allergen-induced airway inflammation and airway responsiveness and inhibits NF-kappaB activity in a murine model of asthma.

Naringenin, a flavonoid, has antiinflammatory and immunomodulatory properties. We investigated whether naringenin could attenuate allergen-induced airway inflammation and its possible mechanism in a murine model of asthma. Mice were sensitized and challenged with ovalbumin.

Experimental demonstration of high quality MeV ultrafast electron diffraction.

The simulation optimization and an experimental demonstration of improved performances of mega-electron-volt ultrafast electron diffraction (MeV UED) are reported in this paper. Using ultrashort high quality electron pulses from an S-band photocathode rf gun and a polycrystalline aluminum foil as the sample, we experimentally demonstrated an improved spatial resolution of MeV UED, in which the Debye-Scherrer rings of the (111) and (200) planes were clearly resolved. This result showed that MeV UED is capable to achieve an atomic level spatial resolution and a approximately 100 fs temporal resolution simultaneously, and will be a unique tool for ultrafast structural dynamics studies.

MAGE-D1 inhibits proliferation, migration and invasion of human breast cancer cells.

MAGE-D1, also known as NRAGE or Dlxin-1, is a member of the MAGE family of proteins. It interacts with multiple adaptors and mediates various cellular functions such as regulation of apoptosis, transcription, cell cycle, cell adhesion and angiogenesis. In this study, we evaluated the effect of MAGE-D1 plasmid transfection on the growth, migration and invasion of breast cancer cells.

Preactivation of NKT cells with alpha-GalCer protects against hepatic ischemia-reperfusion injury in mouse by a mechanism involving IL-13 and adenosine A2A receptor.

Hepatic preconditioning has emerged as a promising strategy of activating natural pathways to augment tolerance to liver ischemia-reperfusion (IR) injury. Liver-resident natural killer T (NKT) cells play an important role in modulating the local immune and inflammatory responses. This work was aimed to investigate whether preactivation of NKT cells could provide a beneficial "preconditioning" effect to ameliorate the subsequent hepatic IR injury.

Inhibitory effects of sunitinib on ovalbumin-induced chronic experimental asthma in mice.

Background: Tyrosine kinase signaling cascades play a critical role in the pathogenesis of allergic airway inflammation. Sunitinib, a multitargeted receptor tyrosine kinase inhibitor, has been reported to exert potent immunoregulatory, anti-inflammatory and anti-fibrosis effects. We investigated whether sunitinib could suppress the progression of airway inflammation, airway hyperresponsiveness (AHR), and airway remodeling in a murine model of chronic asthma.

Hesperetin inhibits the maturation and function of monocyte-derived dendritic cells from patients with asthma.

Dendritic cells (DCs) are crucial regulators of allergic diseases. Hesperetin, an important bioactive compound in Chinese traditional medicine, has antioxidant and anti-allergic properties. In this study, we examined whether hesperetin influences surface molecule expression, cytokine production, the capacity to induce T cell proliferation, and the underlying signaling pathway in monocyte-derived DCs from patients with allergic asthma.