Features of Thermomechanical Stability of Anionic-Cation Exchange Matrix "Polikon AC" on Viscose Non-Woven Materials.

The thermomechanical stability of the anion-cation exchange matrix "Polikon AC" on viscose nonwoven materials is investigated. In this work, a molecular model of a solvation environment for experimentally obtained "Polikon AC" mosaic membranes is refined. Mosaic membranes on a viscose fiber base were fabricated by the method of polycondensation filling.

Probing the Effect of Molecular Nonuniformity in Directed Self-Assembly of Diblock Copolymers in Nanoconfined Space.

Various complex self-assembled morphologies of lamellar- and cylinder-forming block copolymers comprising poly(dimethylsiloxane)-b-polylactide (PDMS-b-PLA) confined in cylindrical channels were generated. Combining top-down lithography with bottom-up block copolymer self-assembly grants access to morphologies that are otherwise inaccessible with the bulk materials. Channel diameter (D) was systematically varied with four diblock copolymers having different compositions and bulk domain spacing (L0), corresponding to a range of frustration ratios (D/L0 from 2 to 4).

[The synthesis of P1-[11-(anthracen-9-ylmethoxy)undecyl]-P2(2-Acetamido-2-deoxy-α-D-glucopyranosyl) diphosphate and the study of its acceptor properties in the enzymic reaction catalyzed by D-rhamnosyltransferase from Pseudomonas aeruginosa].

P1-[11-(Anthracen-9-ylmethoxy)undecyl]-P2-(2-acetamido-2-deoxy-α-D-glucopyranosyl) diphosphate, a fluorescent derivative of undecyl diphosphate 2-acetamido-2-deoxyglucose, was chemically synthesized. The ability of the compound to serve as acceptor substrate of D-rhamnose residue in the enzymatic reaction catalyzed by D-rhamnosyltransferase from Pseudomonas aeruginosa PAO1 was demonstrated.

[Ultrasound monitoring of consolidation processes in fractures of long tubular bones in osteosynthesis using bioactive implants].

Objective: To show the capabilities of ultrasound monitoring to assess consolidation processes in fractures of long tubular bones in the use of bioactive material-containing implants.

Material And Methods: Eighty-two (45.1%) patients whose bone fragments had been fixed with bioactive material-coated plates and 100 (54.

[Synthesis of 11-[(2-pyridyl)amino]- and 11-[(9-anthracenylcarbonyl)amino]undecyl phosphate and investigation of their acceptor properties in the enzymic reaction catalyzed by galactosylphosphotransferases from Salmonella].

11-[(2-Pyridyl)amino]undecyl phosphate and 11-[(9-anthracenylcarbonyl)amino]undecyl phosphate were chemically synthesized. The abiliy of these new fluorescent derivatives of undecyl phosphate to serve as acceptor substrate of galactosyl phosphate residue in the enzymic reaction catalyzed by galactosylphosphotransferase from Salmonella anatum or Salmonella newport membrane preparation was demonstrated.

Biochemical characterization of a new β-1,3-galactosyltransferase WbuP from Escherichia coli O114 that catalyzes the second step in O-antigen repeating-unit.

In this study, synthetic acceptor substrate GlcNAc alpha-PO3-PO3-(CH2)11-O-phenyl (GlcNAc-PP-PhU) was employed in glycosyl transferase assays to characterize the WbuP galactosyltransferase activity. This activity was time- and enzyme concentration-dependent. The optimal enzyme activity was observed at pH 6.

[Synthesis and acceptor properties of 11-[(9'-anthracenyl)methoxy]undecyl phosphate and P1-{11-[(9'-anthracenyl)methoxy]undecyl}-P2-(alpha-D-galactopyranosyl) diphosphate in the enzymic reactions catalyzed by galactosylphosphotransferase and mannosyltransferase from Salmonella newport].

Fluorescent 11-[(9'-anthracenyl)methoxy]undecyl phosphate and P1-{11-[(9'-anthracenyl)methoxy]undecyl}-P2-(alpha-D-galactopyranosyl) diphosphate were chemically synthesized for the first time. The ability of the first compound to serve as substrate-acceptor ofgalactosyl phosphate residue and the second compound of mannosyl residue in enzymic reactions catalyzed by galactosylphosphotransferase and mannosyltransferase from Salmonella newport membrane preparation was demonstrated.

Hierarchical, guided self-assembly of preselected carbon nanotubes for the controlled fabrication of CNT structures by electrooxidative nanolithography.

A fully controllable process for the fabrication of carbon nanotube assemblies is presented on the basis of a sequential electrochemical oxidation lithography process. This approach utilizes the local chemical conversion of a n-octadecyltrichlorosilane self-assembled monolayer into a template featuring polar acid groups. The capability to utilize such chemically active templates for the site-selective assembly of individual carbon nanotubes was demonstrated, and a hierarchical, sequential structuring routine to obtain crossed CNT configurations, formed by preselected carbon nanotubes, was implemented.

Synthesis of a fluorescent acceptor substrate for glycosyltransferases involved in the assembly of O-antigens of enterohemorrhagic Escherichia coli O157 and O5.

The assembly of the repeating units of O-antigens in Gram negative bacteria is catalyzed by specific glycosyltransferases. Previously we used GlcNAc/GalNAcα-diphosphate-phenoxyundecyl as natural acceptor substrate analogs in assays of the transfer of radioactive sugars by bacterial glycosyltransferases. In order to develop new, fluorescence based assays we have synthesized a fluorescent acceptor P¹-[11-(anthracen-9-ylmethoxy)undecyl]-P²-(2-acetamido-2-deoxy-α-D-galactopyranosyl) diphosphate and have shown that the compound was an excellent acceptor for glucosyltransferase WbdN from Escherichia coli (E.

[Synthesis of p(1)-(11-phenoxyundecyl)-P(2)-(alpha-D-galactopyranosyl) diphosphate and p(1)-(11-phenoxyundecyl)-P(2)-(alpha-D-glucopyranosyl) diphosphate; assay of their acceptor properties for mannosyl residue in the enzmic reaction catalyzed by mannosyltransferase from Salmonella newport].

P(1)-(Phenoxyundecyl)-P(2)-(alpha-D-galactopyranosyl) diphosphate as well as P(1)-(11-phenoxyundecyl)-P(2)-(alpha-D-glucopyranosyl) diphosphate are newly synthesized and their ability to serve as substrate-acceptor of mannosyl residue in enzymic reaction catalyzed by mannosyltransferase from Salmonella newport membrane preparation is investigated. The possibility ofgalactosyl-containing derivative to serve as mannosyl acceptor from GDP-Man is established whereas glucosyl-containing compound is inactive in this process.

Biochemical characterization of WbdN, a β1,3-glucosyltransferase involved in O-antigen synthesis in enterohemorrhagic Escherichia coli O157.

The enterohemorrhagic O157 strain of Escherichia coli, which is one of the most well-known bacterial pathogens, has an O-antigen repeating unit structure with the sequence [-2-d-Rha4NAcα1-3-l-Fucα1-4-d-Glcβ1-3-d-GalNAcα1-]. The O-antigen gene cluster of E. coli O157 contains the genes responsible for the assembly of this repeating unit and includes wbdN.

Tissue regeneration in vivo within recombinant spidroin 1 scaffolds.

One of the major tasks of tissue engineering is to produce tissue grafts for the replacement or regeneration of damaged tissue, and natural and recombinant silk-based polymer scaffolds are promising candidates for such grafts. Here, we compared two porous scaffolds made from different silk proteins, fibroin of Bombyx mori and a recombinant analog of Nephila clavipes spidroin 1 known as rS1/9, and their biocompatibility and degradation behavior in vitro and in vivo. The vascularization and intergrowth of the connective tissue, which was penetrated with nerve fibers, at 8 weeks after subcutaneous implantation in Balb/c mice was more profound using the rS1/9 scaffolds.

[Simple synthesis of P¹-(11-phenoxyundecyl)-P²-(2-acetamido-2-deoxy-α-D-galactopyranosyl) diphosphate].

A simple scheme of synthesis of P¹-(11-phenoxyundecyl)-P²-(2-acetamido-2-deoxy-α-D-galactopyranosyl) diphosphate synthetic lipid acceptor for glycosyltransferases participating in gram-negative bacteria O-antigenic polysaccharides is suggested.

Strategies for post-synthesis alignment and immobilization of carbon nanotubes.

Carbon nanotubes (CNTs) have developed into a standard material used as a building block for nanotechnological developments. Based on the unique properties that make CNTs useful for many different applications in nanotechnology, optics, electronics, and material science, there has been a rapid development of this research area and many different applications have emerged in the past few years. Frequently, the alignment and immobilization of CNTs play an important role for many applications and different strategies, in particular post-synthesis approaches, can be applied.

11-Phenoxyundecyl phosphate as a 2-acetamido-2-deoxy-α-d-glucopyranosyl phosphate acceptor in O-antigen repeating unit assembly of Salmonella arizonae O:59.

A synthesis of 11-phenoxyundecyl phosphate and its biochemical transformation (using GlcNAc-P transferase from Salmonella arizonae O:59 membranes catalysing transfer of GlcNc-phosphate from UDP-GlcNAc on lipid-phosphate) into P(1)-11-phenoxyundecyl, P(2)-2-acetamido-2-deoxy-α-D-glucopyranosyl diphosphate are described.

Microwave-assisted fabrication of carbon nanotube AFM tips.

A new, fast, alternative approach for the fabrication of carbon nanotube (CNT) atomic force microscopy (AFM) tips is reported. Thereby, the tube material is grown on the apex of an AFM tip by utilizing microwave irradiation and selective heating of the catalyst. Reaction times as short as three minutes allowed the fabrication of CNT AFM tips in a highly efficient process.

Mutation in the pssM gene encoding ketal pyruvate transferase leads to disruption of Rhizobium leguminosarum bv. viciae-Pisum sativum symbiosis.

Aims: To study the question whether acidic exopolysaccharide (EPS) modification, e.g. pyruvylation, plays any role in the development of Rhizobium leguminosarum/Pisum sativum symbiosis.

[Synthesis of 11-phenoxyundecyl phosphate and its use as an acceptor substrate in the reaction with UDP-GlcNAc : polyprenyl phosphate GlcNAc-phosphotransferase from Salmonella arizona O:59].

A new scheme of synthesis of 11-phenoxyundecyl phosphate from 11-bromoundecanoic acid was suggested for its ability to react as an acceptor of 2-acetamido-2-deoxy-alpha-D-glucopyranosyl phosphate in a reaction catalyzed by UDP-N-acetylglucosamine : polyprenyl phosphate N-acetylglucosamine phosphotransferase from Salmonella arizona O:59.

[Assessment of the level of immunoglobulin E in patients with pyo-inflammatory diseases and its influence on the course of the infectious process].

The level of immunoglobulin E (IgE) in patients with different pyo-inflammatory diseases was assessed and it was found to elevate in patients with acute appendicitis in 49% of cases, in patients with chronic relapsing furunculosis (CRF) in 41%, with chronic osteomyelitis--in 66.6%. In 8 out of 16 examined patients with the elevated level of IgE there were antibodies to toxocars in titers 1:800, 1:400.

Chemical synthesis of dolichyl phosphates, their analogues and derivatives and application of these compounds in biochemical assays.

Several methods for simple and efficient chemical synthesis of dolichyl phosphates and their analogues and derivatives are briefly summarized with a special emphasis on chemical modification of phosphoryl group and preparation of dolichyl phosphates labelled at the omega-end and at the gamma-isoprene unit of the isoprene chain by fluorescent groups, 2-aminopyridine and 1-aminonaphtalene residues. Additionally, data on biochemical assays with application of the compounds mentioned above are presented.

[The pssA gene encodes UDP-glucose: polyprenyl phosphate-glucosyl phosphotransferase initiating biosynthesis of Rhizobium leguminosarum exopolysaccharide].

Symbiotic nitrogen-fixing bacteria Rhizobium leguminosarum by. viciae VF39 secrete an acidic heteropolysaccharide, the biosynthesis of which involves the stage of polyprenyl diphosphate octasaccharide formation, with its carbohydrate fragment corresponding to the repeating polymer unit. The amino acid analysis of the product of the pssA gene, we have earlier identified, showed its homology to bacterial polyisoprenyl phosphate hexose 1-phosphate transferases catalyzing the formation of phosphodiester bonds between polyprenyl phosphates and hexose 1-phosphates, whose donors are nucleotide sugars.