Pleiotropic effect of anionic phospholipids absence on mitochondrial morphology and cell wall integrity in strictly aerobic Kluyveromyces lactis yeasts.

Cardiolipin and phosphatidylglycerol are anionic phospholipids localized to the inner mitochondrial membrane. In this study, it is demonstrated by fluorescence and transmission electron microscopy that atp2.1pgs1Δ mutant mitochondria lacking anionic phospholipids contain fragmented and swollen mitochondria with a completely disorganized inner membrane.

Production of squalene by lactose-fermenting yeast Kluyveromyces lactis with reduced squalene epoxidase activity.

Unlabelled: Utilization of yeast as squalene source for commercial use is limited by relatively high production costs. The ability of Kluyveromyces lactis to grow on cheap lactose-containing diary industry wastes could improve the economy of the production process. We therefore tested the potential of this yeast for squalene production.