[Immunobiological properties of monoclonal antibodies to Mycoplasma hominis antigens].

Approaches to obtaining stable mouse hybridomas synthesizing monoclonal antibodies (McAb) to M. hominis key antigens were developed. 4 clones capable of the stable synthesis of McAb of different IgG classes were obtained.

[Monoclonal antibodies to Mycoplasma pneumoniae antigens].

Approaches to obtaining stable mouse hybridomas, capable of producing monoclonal antibodies (McAb) to M. pneumoniae key antigens, were developed. As the result of hybridization experiments, 7 clones were obtained; of these, 4 clones stably synthesized IgG McAb.

[Immunodiagnostic preparations based on monoclonal antibodies to Chlamydia trachomatis].

Highly sensitive diagnostic preparations for the detection of C. trachomatis by direct immunofluorescent and enzyme immunoassay techniques were obtained with the use monoclonal antibodies to C. trachomatis genus-specific polysaccharide antigen.

[The immunochemical and biological properties of monoclonal antibodies to Chlamydia trachomatis].

In this work some properties of 11 monoclonal antibodies to C.trachomatis, obtained in our earlier investigations, were studied and the antigens recognized by these McAb were characterized. Some McAb reacted with a genus-specific thermostable antigen, sensitive to sodium metaperiiodate and having a mol.

[Multispecific monoclonal antibodies].

Immunization of BALB/c mice with Rickettsia prowazekii antigens, Bordetella pertussis toxin and Legionella pneumophila cytolysin induces the synthesis of IgM autoantibodies of different specificity. Among monoclonal antibodies, multispecific antibodies with a wide reactivity spectrum have been found to make up high percentage (30-80%). Monoclonal antibodies interact with different bacterial antigens and tissue substances.

[The typing of Legionella pneumophila strains by using monoclonal antibodies to the cytolysin].

Studies on the typing of L. pneumophila strains of serogroup 1, isolated from patients and environmental objects, have been made with the use of monoclonal antibodies (McAb) to cytolysin. The results of the comparison of the specificity of our McAb with that of a commercial set McAb obtained from the USA make it possible to recommend preparations based on McAb to cytolysin for the detection of L.

[The biological properties of monoclonal antibodies to the cytolysin of Legionella pneumophila].

The study of the biological properties of monoclonal antibodies (McAb) to L. pneumophila cytolysin has been carried out. These McAb have been shown to possess no capacity for the in vitro neutralization of cytolysin and the protection of guinea pigs from aerosol infection with L.

[The detection of Rickettsia prowazekii in the vectors of louse-born typhus Pediculus humanus by using monoclonal antibody-based preparations].

The results of this investigation indicate that preparations obtained on the basis of monoclonal antibodies have proved to be suitable for the detection of R. prowazekii antigens in the natural carrier of typhus when used in all types of the enzyme immunoassay; of these, the assay made by the capture method has been found to possess the highest sensitivity. The testing of the sensitivity limits has shown that this method known as ELISA-mu-capture, i.

[The surface antigens of Rickettsia prowazekii studied with monoclonal antibodies].

R. prowazekii antigens have been tested with the use of monoclonal antibodies (McAb) to different epitopes of the microorganism. As revealed in these tests, McAb B4/4 and A-3/D, active against species-specific thermolabile antigen, interact with protein having a molecular weight of 90-120 KD.

[Monoclonal antibodies to the species-specific and group antigens of Rickettsia prowazekii].

A number of hybridomas to different R. prowazekii determinants were obtained by the hybridization of spleen cells of BALB/c mice immunized with R. prowazekii corpuscular and soluble antigens.

[Monoclonal antibodies to Legionella pneumophila cytolysin].

The fusion of spleen cells, taken from BALB/c mice immunized with the purified preparation of L. pneumophila cytolysin, with cells Sp2/0 and NP has been carried out. As a result, hybridoma cells producing IgG1, IgG3 and IgM antibodies to this protein have been obtained.

[Monoclonal antibodies cross-reacting with fibroblasts of interstitial connective tissue of the myocardium and cell wall protein antigens of group A Streptococcus].

Monoclonal antibodies (MCA) B6/5 and C5/3 were obtained after immunization of BALB/c mice with the protein non-type-specific antigens (NTSA) of streptococcal group A cell wall. MCA B6/5 in the indirect immunofluorescence react with human and animal interstitial connective tissue (ICT) of the myocardium and human fibroblast culture cells. MCA C5/3 react with the bands of muscle fibers of the myocardium.

[Monoclonal autoantibodies to the epithelial basement membrane cells of human skin and thymus obtained through immunization with Rickettsia prowazekii antigens].

As the result of immunization of BALB/c mice with the commercial preparation of typhus vaccine and R. prowazekii corpuscular antigen, in 29.2% and 40.

[Ultraimmunocytochemical diagnosis of squamous cell cancer of the digestive tract].

Procedure using fluorescent and peroxidase labeling and monoclonal antibodies to basal-cell antigen of multilayer epithelium showed the antigen to occur in squamous cell cancer irrespective of site and degree of cell differentiation; this, however, was not the case with tumors of other origins. Ultraimmunocytochemical method was used to identify localization of the antigen at cellular and subcellular levels, namely, in tonofilaments and desmosomes. Said situation is considered to suggest squamous cell carcinoma.

[Monoclonal autoantibodies to different epithelial structures of the thymus gland obtained by the immunization with streptococcal group A antigens].

By the indirect immunofluorescence method it was shown to which epithelial thymus structures monoclonal antibodies (mAT) reacting with the different epidermal structures are directed. These mAT related to the autoantibodies were obtained earlier, as a result of lymphoid cells polyclonal activation, by the immunization of BALB/c mice with streptococcal group A nonspecific protein antigens of the cell wall. It was shown that mAT A6/1, reacting with the basal layer of the skin epithelium are directed to the epithelium of the cortical and medullar thymus zones, which is regarded as the so called endocrinal epithelium.

[Characteristics of monoclonal antibodies directed to species specific and group specific antigens of Rickettsia prowazekii].

A number of hybridomas against different determinants of R. prowazekii were obtained by hybridization of splenocytes from BALB/c mice immunized with whole cells or soluble antigens of the organism. Some of the monoclonal antibodies bound to thermolabile species specific protein of R.

[The localization of structurally specific antigens in squamous cell skin cancer using monoclonal antibodies to epidermal antigens].

Immunomorphological studies by indirect immunofluorescent method using monoclonal antibodies to antigens of basal cells, suprabasal cells and basal membrane of the epidermis were carried out in 8 cases of squamous-cell skin cancer. Specific characteristics of localization of structure-specific antigens (as compared with normal human skin) in squamous-cell skin cancer are discussed.

[Monoclonal antibodies to polysaccharides of group A streptococci cross reacting with mammalian connective tissue].

Monoclonal antibodies (MCA) D 4/1 were obtained by immunization of BALB/c mice with group A streptococci treated with pepsin. MCA D 4/1 react in ELISA test with streptococcal group A, C, L, and A-variant (V) polysaccharides (PS). D 4/1 are autoantibodies, cross-reacting in immunofluorescent test with all the epithelial layers and fibroblast-like structures of human and BALB/c mouse skin.

[Production of monoclonal antibodies to tissue-specific antigens of human skin epithelium].

Immunization of BALB/c mice with non-type-specific protein antigens of the cellular wall of group A streptococcus and formalin-treated streptococcal culture resulted in stimulating production of polyclonal autoantibodies to antigens of the epithelium of human and murine skin. As a result of hybridoma technique using splenic cells of immunized animals, monoclonal antibodies to different antigens of epidermal cell cytoplasm, i.e.

[Reaction of monoclonal antibodies A 6/1 with the antigen determinant of ultrastructures of tumor cells in epidermal-type tegmental tissues].

Using indirect immunoenzyme assay and ultrathin sections, it was shown that antibodies against basal cell antigens react with tonofibril and desmosome filaments of squamous tissue carcinoma cells.

[Preparation of monoclonal antibodies to nuclear DNA of mammalian cells by immunization with group A streptococcal polysaccharide conjugated with synthetic polyelectrolytes].

Monoclonal antibodies (MAb) were obtained by hybridization of spleen cells from BALB/c mice immunized with streptococcal group A polysaccharide (A-PS) conjugated with synthetic polyelectrolytes (PEL). These MAb reacted with nuclei from human and mouse cells. MAb reacting with nuclei were obtained after prolonged immunization with conjugates and were not formed by hybridization of spleen cells from non-immunized mice or by the immunization with PEL.

[Trial of pertussis toxin activity in vitro on CHO cells].

The activity of B. pertussis toxin has been tested in the continuous culture of CHO (Chinese hamster ovary) cells. The in vitro method of testing B.

[Monoclonal antibodies against group A streptococcal polysaccharide reacting with basal-cell antigen of tumors histogenetically related to epidermal tissues].

Immunofluorescence of tumour tissues histogenetically related to tissues containing cross-reacting squamous epithelial basal-cell antigen (BCAg) was performed using monoclonal antibodies (McAb) A6/I-D to squamous epithelial basal-cell antigen cross-reacting with group A staphylococcal polysaccharide. BCAg was found in tumour cells arising from surface tissues of the epidermal type (basal-cell cancer, squamous-cell cancer of the skin, esophagus, cervix uteri and other organs) and was not found in glandular tumours (adenocarcinoma of the stomach, intestine, mammary glands). The results obtained indicate that McAb A6/I-D against cross-reacting BCAg may be used while characterizing the majority of normal epidermal tissues and tumours arising from tissues containing cross-reacting BCAg.