Molecular epidemiology of tuberculosis in Prague and South Moravia, Czech Republic: genetic analysis of Mycobacterium tuberculosis isolates by IS6110-RFLP fingerprinting and spoligotyping.

Objectives: To genetically characterize and compare Mycobacterium tuberculosis isolates among culture-confirmed TB cases in two regions in the Czech Republic in 1998.

Methods: Consecutive M. tuberculosis isolates from 111 TB patients in Prague and 120 patients in the South Moravia region were genotyped using the standardized IS6110 Southern blot hybridization method and by spoligotyping.

Postpartum depression as profiled through the depression screening scale.

Purpose: To describe in greater depth the profiles of the dimensions of the Postpartum Depression Screening Scale (PDSS), with a focus on those women identified with postpartum depression (PPD), and to provide a discussion of the implications for early detection in the community.

Method And Design: Subanalysis of data from 150 new mothers who completed the PDSS and had a DSM-IV diagnostic interview conducted by a nurse psychotherapist. Data analysis focused on exploring the profiles of women who were diagnosed with PPD as well as those who were not.

Potential nosocomial exposure to Mycobacterium tuberculosis from a bronchoscope.

Objective: To investigate a possible nosocomial outbreak of tuberculosis (TB).

Design: Retrospective cohort study.

Setting: Community hospital.

Optimization of CH fluorescence diagnostics in flames: range of applicability and improvements with hydrogen addition.

This study quantifies the range of premixed flame conditions for which CH fluorescece diagnostics are applicable, and it shows that the CH fluorescence signal can be increased if some of the hydrocarbon fuel is replaced with hydrogen. The CH fluorescence signal is found to be adequate for fuel-air equivalence ratios (phi) as small as 0.85 for both methane-air and propane-air flames.

Snapshot of moving and expanding clones of Mycobacterium tuberculosis and their global distribution assessed by spoligotyping in an international study.

The present update on the global distribution of Mycobacterium tuberculosis complex spoligotypes provides both the octal and binary descriptions of the spoligotypes for M. tuberculosis complex, including Mycobacterium bovis, from >90 countries (13,008 patterns grouped into 813 shared types containing 11,708 isolates and 1,300 orphan patterns). A number of potential indices were developed to summarize the information on the biogeographical specificity of a given shared type, as well as its geographical spreading (matching code and spreading index, respectively).

Arthroscopic posterior cruciate ligament repair for acute femoral "peel off" tears.

Posterior cruciate ligament (PCL) injuries can be associated with acute and chronic morbidity. Treatment of PCL disruption is typically either nonoperative or reconstructive, using a graft substitute. We describe a minimally invasive arthroscopic technique for repair of acute PCL tears of the femoral origin.

Global distribution of Mycobacterium tuberculosis spoligotypes.

We present a short summary of recent observations on the global distribution of the major clades of the Mycobacterium tuberculosis complex, the causative agent of tuberculosis. This global distribution was defined by data-mining of an international spoligotyping database, SpolDB3. This database contains 11708 patterns from as many clinical isolates originating from more than 90 countries.

Spoligologos: a bioinformatic approach to displaying and analyzing Mycobacterium tuberculosis data.

Spacer oligonucleotide (spoligotyping) analysis is a rapid polymerase chain reaction-based method of DNA fingerprinting the Mycobacterium tuberculosis complex. We examined spoligotype data using a bioinformatic tool (sequence logo analysis) to elucidate undisclosed phylogenetic relationships and gain insights into the global dissemination of strains of tuberculosis. Logo analysis of spoligotyping data provides a simple way to describe a fingerprint signature and may be useful in categorizing unique spoligotypes patterns as they are discovered.

Impact of genotyping of Mycobacterium tuberculosis on public health practice in Massachusetts.

Massachusetts was one of seven sentinel surveillance sites in the National Tuberculosis Genotyping and Surveillance Network. From 1996 through 2000, isolates from new patients with tuberculosis (TB) underwent genotyping. We describe the impact that genotyping had on public health practice in Massachusetts and some limitations of the technique.

Estimated costs of false laboratory diagnoses of tuberculosis in three patients.

We estimated direct medical and nonmedical costs associated with a false diagnosis of tuberculosis (TB) caused by laboratory cross-contamination of Mycobacterium tuberculosis cultures in Massachusetts in 1998 and 1999. For three patients who received misdiagnoses of active TB disease on the basis of laboratory cross-contamination, the costs totaled U.S.

Genotyping analyses of tuberculosis cases in U.S.- and foreign-born Massachusetts residents.

We used molecular genotyping to further understand the epidemiology and transmission patterns of tuberculosis (TB) in Massachusetts. The study population included 983 TB patients whose cases were verified by the Massachusetts Department of Public Health between July 1, 1996, and December 31, 2000, and for whom genotyping results and information on country of origin were available. Two hundred seventy-two (28%) of TB patients were in genetic clusters, and isolates from U.

Molecular epidemiology of multidrug-resistant tuberculosis, New York City, 1995-1997.

From January 1, 1995, to December 31, 1997, we reviewed records of all New York City patients who had multidrug-resistant tuberculosis (MDRTB); we performed insertion sequence (IS) 6110-based DNA genotyping on the isolates. Secondary genotyping was performed for low IS6110 copy band strains. Patients with identical DNA pattern strains were considered clustered.

Sputum induction problems identified through genetic fingerprinting.

Objective: To identify the contamination source of a cluster of eight positive Mycobacterium tuberculosis isolates from one laboratory session.

Methods: Spoligotyping was performed on M. tuberculosis isolates processed during one laboratory session.

Clonal relationships in a shelter-associated outbreak of drug-resistant tuberculosis: 1983-1997.

Setting: An outbreak of tuberculosis caused by Mycobacterium tuberculosis resistant to isoniazid and streptomycin (HS-resistant) was documented in Boston's homeless population in 1984. Isolate relatedness was confirmed at the time by phage typing. In the late 1990s, cases of HS-resistant tuberculosis in the homeless were also documented, confirmed by RFLP typing using IS6110.

A light in the fog: caring for women with postpartum depression.

It is estimated that at least 1 in 10 women will experience postpartum depression, yet systematic screening for it in clinical practice is too often neglected. The foggy unreality of this affective disorder leads women to believe they are losing their minds, and their efforts to find help can be elusive. Women with postpartum depression who go undetected and untreated are at risk for immediate harm and potential lifelong sequelae for themselves and their families, and especially for their children.

Paradigms for assessment: women's knowledge and skill attainment.

This article describes two models of assessment that nurses can use with women throughout their life span: (1) knowledge attainment (Belenky, Clinchy, Goldberger, & Tarule, 1986) and (2) skill attainment (Dreyfus & Dreyfus, 1996). These models consider each woman's unique reality, acknowledging that how she experiences pregnancy and postpartum is based on how she has lived her life. Her integration of experiences, coping strategies, and life events may be the foundation upon which she will incorporate the new experiences of pregnancy and motherhood.

[Spoligotypes of Mycobacterium tuberculosis in the Czech population (methods and pilot study)].

In the introduction the authors describe molecular genetic principles of spacer oligonucleotide typing of the M. tuberculosis complex and mention in detail the methodical procedure of implementation of this examination technique incl. computer analysis of results.

Identification and evolution of an IS6110 low-copy-number Mycobacterium tuberculosis cluster.

A cohort of 56 patients infected with related strains of Mycobacterium tuberculosis, the S75 group, was identified in a New Jersey population-based study of all isolates with a low number of copies of the insertion element IS6110. Genotyping was combined with surveillance data to identify the S75 group and to elucidate its recent evolution. The S75 group had similar demographic and geographic characteristics.

Molecular identification of streptomycin monoresistant Mycobacterium tuberculosis related to multidrug-resistant W strain.

A distinct branch of the Mycobacterium tuberculosis W phylogenetic lineage (W14 group) has been identified and characterized by various genotyping techniques. The W14 group comprises three strain variants: W14, W23, and W26, which accounted for 26 clinical isolates from the New York City metropolitan area. The W14 group shares a unique IS6110 hybridizing banding motif as well as distinct polymorphic GC-rich repetitive sequence and variable number tandem repeat patterns.