Antibody Design with SE(3) Diffusion.

We introduce , an antibody variable domain diffusion model based on a general protein backbone diffusion framework, which was extended to handle multiple chains. Assessing the designability and novelty of the structures generated with our model, we find that produces highly designable antibodies that can contain novel binding regions. The backbone dihedral angles of sampled structures show good agreement with a reference antibody distribution.

Large scale paired antibody language models.

Antibodies are proteins produced by the immune system that can identify and neutralise a wide variety of antigens with high specificity and affinity, and constitute the most successful class of biotherapeutics. With the advent of next-generation sequencing, billions of antibody sequences have been collected in recent years, though their application in the design of better therapeutics has been constrained by the sheer volume and complexity of the data. To address this challenge, we present IgBert and IgT5, the best performing antibody-specific language models developed to date which can consistently handle both paired and unpaired variable region sequences as input.

ABodyBuilder3: improved and scalable antibody structure predictions.

Summary: In this article, we introduce ABodyBuilder3, an improved and scalable antibody structure prediction model based on ABodyBuilder2. We achieve a new state-of-the-art accuracy in the modelling of CDR loops by leveraging language model embeddings, and show how predicted structures can be further improved through careful relaxation strategies. Finally, we incorporate a predicted Local Distance Difference Test into the model output to allow for a more accurate estimation of uncertainties.

A chip-based C-band ODNP platform.

In this paper, we present a chip-based C-band ODNP platform centered around an NMR-on-a-chip transceiver and a printed microwave (MW) Alderman-Grant (AG) coil with a broadband tunable frequency range of 528MHz. The printable ODNP probe is optimized for a high input-power-to-magnetic-field conversion-efficiency, achieving a measured ODNP enhancement factor of -151 at microwave power levels of 33.3dBm corresponding to 2.

Being right vs. getting it right: orientation to being recorded in psychotherapeutic interaction as disaffiliative vs. affiliative practice.

Introduction: The study focuses on the orientation to being recorded in therapy sessions, emphasizing that these practices adapt to specific circumstances and influence subsequent actions. The study suggests a way to deal with the insolubility of the "observer paradox": to accept that observation has an impact on the observed, but that the recorder is not necessarily a negative determinant. Furthermore, the study builds on the idea that participants' orientations to the recorder can be seen as actions.

A Portable Chip-Based NMR Relaxometry System With Arbitrary Phase Control for Point-of-Care Blood Analysis.

In this article, we present a portable NMR relaxometry system optimized for the point-of-care analysis of body liquids such as blood. The presented system is centered on an NMR-on-a-chip transceiver ASIC, a reference frequency generator with arbitrary phase control, and a custom-designed miniaturized NMR magnet with a field strength of 0.29 T and a total weight of 330 g.

Direct access to millions of mutations by whole genome sequencing of an oilseed rape mutant population.

Induced mutations are an essential source of genetic variation in plant breeding. Ethyl methanesulfonate (EMS) mutagenesis has been frequently applied, and mutants have been detected by phenotypic or genotypic screening of large populations. In the present study, a rapeseed M population was derived from M parent cultivar 'Express' treated with EMS.

Mapping-by-Sequencing Reveals Genomic Regions Associated with Seed Quality Parameters in .

Rapeseed ( L.) is an important oil crop and has the potential to serve as a highly productive source of protein. This protein exhibits an excellent amino acid composition and has high nutritional value for humans.

[Implantation of a pseudo knee prosthesis after rotationplasty: a case report].

Rotationplasty of the knee joint is an established biological reconstruction method with excellent clinical results for patients with tumor-associated resection of the knee joint. The changed biomechanics of the pseudo knee and high demand of the generally young patients commonly leads to the development of osteoarthritic changes in this area. We report on the implantation of an ankle prosthesis in the area of the pseudo knee joint, which made it possible to use a conventional exoprosthesis.

A portable NMR platform with arbitrary phase control and temperature compensation.

In this paper, we present a custom-designed nuclear magnetic resonance (NMR) platform based on a broadband complementary metal-oxide-semiconductor (CMOS) NMR-on-a-chip transceiver and a synchronous reference signal generator, which features arbitrary phase control of the excitation pulse in combination with phase-coherent detection at a non-zero intermediate frequency (IF). Moreover, the presented direct digital synthesis (DDS)-based frequency generator enables a digital temperature compensation scheme similar to classical field locking without the need for additional hardware. NMR spectroscopy and relaxometry measurements verify the functionality of the proposed frequency reference and temperature compensation scheme as well as the overall state-of-the-art performance of the presented system.

Two wrongs make a right: heat stress reversion of a male-sterile Brassica napus line.

Male-sterile lines play important roles in plant breeding to obtain hybrid vigour. The male sterility Lembke (MSL) system is a thermosensitive genic male sterility system of Brassica napus and is one of the main systems used in European rapeseed breeding. Interestingly, the MSL system shows high similarity to the 9012AB breeding system from China, including the ability to revert to fertile in high temperature conditions.

Lesion-symptom mapping of language impairments in patients suffering from left perisylvian gliomas.

Brain tumors cause local structural impairments of the cerebral network. Moreover, brain tumors can also affect functional brain networks more distant from the lesion. In this study, we analyzed the impact of glioma WHO grade II-IV tumors on grey and white matter in relation to impaired language function.

Long-Term Stability of Short-Term Intensive Language-Action Therapy in Chronic Aphasia: A 1-2 year Follow-Up Study.

Intensive aphasia therapy can improve language functions in chronic aphasia over a short therapy interval of 2-4 weeks. For one intensive method, , beneficial effects are well documented by a range of randomized controlled trials. However, it is unclear to date whether therapy-related improvements are maintained over years.

Detecting Corticospinal Tract Impairment in Tumor Patients With Fiber Density and Tensor-Based Metrics.

Tumors infiltrating the motor system lead to significant disability, often caused by corticospinal tract injury. The delineation of the healthy-pathological white matter (WM) interface area, for which diffusion magnetic resonance imaging (dMRI) has shown promising potential, may improve treatment outcome. However, up to 90% of white matter (WM) voxels include multiple fiber populations, which cannot be correctly described with traditional metrics such as fractional anisotropy (FA) or apparent diffusion coefficient (ADC).

Progress in miniaturization and low-field nuclear magnetic resonance.

In this paper, we review the latest developments in miniaturization of NMR systems with an emphasis on low-field NMR. We briefly cover the topics of magnet and coil miniaturization, elaborating on the advantages and disadvantages of miniaturized coils for different applications. The main part of the article is dedicated to progress in NMR electronics.

Network- and systems-based re-engineering of dendritic cells with non-coding RNAs for cancer immunotherapy.

Dendritic cells (DCs) are professional antigen-presenting cells that induce and regulate adaptive immunity by presenting antigens to T cells. Due to their coordinative role in adaptive immune responses, DCs have been used as cell-based therapeutic vaccination against cancer. The capacity of DCs to induce a therapeutic immune response can be enhanced by re-wiring of cellular signalling pathways with microRNAs (miRNAs).

Support vector machine based aphasia classification of transcranial magnetic stimulation language mapping in brain tumor patients.

Repetitive TMS (rTMS) allows for non-invasive and transient disruption of local neuronal functioning. We used machine learning approaches to assess whether brain tumor patients can be accurately classified into aphasic and non-aphasic groups using their rTMS language mapping results as input features. Given that each tumor affects the subject-specific language networks differently, resulting in heterogenous rTMS functional mappings, we propose the use of machine learning strategies to classify potential patterns of rTMS language mapping results.

Precise predictions for double-Higgs production via vector-boson fusion.

Theoretical predictions with next-to-next-to-leading order (NNLO) QCD accuracy combined with the next-to-leading order (NLO) electroweak (EW) corrections are presented for differential observables of the double-Higgs production process via vector-boson fusion. While the QCD corrections were previously known, the EW ones are computed here for the first time. The numerical results are obtained for a realistic experimental set-up at the LHC and are presented in the form of fiducial cross sections and differential distributions.

Increased Recruitment of Domain-General Neural Networks in Language Processing Following Intensive Language-Action Therapy: fMRI Evidence From People With Chronic Aphasia.

Purpose This study aimed to provide novel insights into the neural correlates of language improvement following intensive language-action therapy (ILAT; also known as constraint-induced aphasia therapy). Method Sixteen people with chronic aphasia underwent clinical aphasia assessment (Aachen Aphasia Test [AAT]), as well as functional magnetic resonance imaging (fMRI), both administered before (T1) and after ILAT (T2). The fMRI task included passive reading of single written words, with hashmark strings as visual baseline.

Parton Showers beyond Leading Logarithmic Accuracy.

Parton showers are among the most widely used tools in collider physics. Despite their key importance, none so far have been able to demonstrate accuracy beyond a basic level known as leading logarithmic order, with ensuing limitations across a broad spectrum of physics applications. In this Letter, we propose criteria for showers to be considered next-to-leading logarithmic accurate.

Incidence and linguistic quality of speech errors: a comparison of preoperative transcranial magnetic stimulation and intraoperative direct cortex stimulation.

Objective: Given the interindividual variance of functional language anatomy, risk prediction based merely on anatomical data is insufficient in language area-related brain tumor surgery, suggesting the need for direct cortical and subcortical mapping during awake surgery. Reliable, noninvasive preoperative methods of language localization hold the potential for reducing the necessity for awake procedures and may improve patient counseling and surgical planning. Repetitive navigated transcranial magnetic stimulation (rnTMS) is an evolving tool for localizing language-eloquent areas.

Chromosome-Scale Assembly of Winter Oilseed Rape .

Rapeseed (), the second most important oilseed crop globally, originated from an interspecific hybridization between and . After this genome collision, underwent extensive genome restructuring, via homoeologous chromosome exchanges, resulting in widespread segmental deletions and duplications. Illicit pairing among genetically similar homoeologous chromosomes during meiosis is common in recent allopolyploids like , and post-polyploidization restructuring compounds the difficulties of assembling a complex polyploid plant genome.

The functional relevance of dorsal motor systems for processing tool nouns- evidence from patients with focal lesions.

This study asks whether lesions in different parts of the brain have different effects on the processing of words typically used to refer to objects with and without action affordances, for example tools and animal-related nouns. A cohort of neurological patients with focal lesions participated in a lexical decision paradigm where nouns semantically related to tools, foods and animals were presented along with matched pseudo-words. Differences in semantic features between the categories were confirmed using extensive semantic ratings whereas all semantic word categories were matched for relevant psycholinguistic variables.