Publications by Drew M Hansen

Publications by authors named "Drew M Hansen"

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A mobile CRISPRi collection enables genetic interaction studies for the essential genes of Escherichia coli.

Kenneth Rachwalski Megan M Tu Sean J Madden Shawn French Drew M Hansen

Cell Rep Methods

January 2024

Advances in gene editing, in particular CRISPR interference (CRISPRi), have enabled depletion of essential cellular machinery to study the downstream effects on bacterial physiology. Here, we describe the construction of an ordered E. coli CRISPRi collection, designed to knock down the expression of 356 essential genes with the induction of a catalytically inactive Cas9, harbored on the conjugative plasmid pFD152.

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