Porosity Effects on the Composite Girder by Rheological Dynamics and FEM.

A theoretical model for porous viscoelastoplastic (VEP) materials in the dry state is investigated in this research study. The model is based on the principles of conservation of mass and energy using the rheological dynamic theory (RDT). The model provides expressions for the creep coefficient, Poisson's ratio, modulus of elasticity, damage variable, and strength as a function of porosity and/or void volume fraction (VVF).

Highly suppressible expression of single-chain interleukin-12 by doxycycline following adenoviral infection with a single-vector Tet-regulatory system.

Background: Adenoviral vectors have been shown to efficiently transfer DNA into a wide variety of eukaryotic cells in vitro and in vivo. However, the therapeutic benefit of this approach is limited by severe side effects as a result of uncontrolled transgene expression.

Methods: A bi-directional promoter that controls the desired transgene as well as a tetracycline-suppressible transactivator (tTA) was cloned into the E1-region of E1-deleted recombinant adenoviral vectors.

Amplified Muc1-specific gene expression in colon cancer cells utilizing a binary system in adenoviral vectors.

Mucin-1 is expressed in a variety of colon carcinomas and Muc-1/DF3 promoters have been utilized to reduce systemic toxicity through specific gene expression. To overcome weak expression, which is much lower than the widely used cytomegalovirus-promoter (CMV), new adenoviral vectors containing a binary system of transgene amplification have been developed. The Muc-1/DF3 promoter was used to control the expression of a Gal4VP16 fusion protein.