[Effects of platelet single passage on coronary circulation in the isolated guinea-pig heart: focus on the L-arginine: nitric oxide system].

Introduction: In order to evaluate endothelial response to platelet single passage through coronary vascular bed, experiments on isolated guinea-pig hearts perfused by the Langendorff method at constant pressure were performed.

Material And Methods: Platelet single passage was performed with platelet-rich plasma obtained from healthy volunteers, as well as from patients with acute myocardial infarction. Hearts, isolated from guinea-pigs of either sex were perfused with Krebs-Hensenleit buffer.