[Early postoperative acute cholecystitis].

Among the relatively rare complications occurring after various operations acute cholecystitis attracts particular attention. Its genesis is elucidated differently in the literature. In addition to the literature information discussed in the work, the authors, possessing experience in the treatment of 14 patients with such postoperative cholecystitis, give a clinical characteristic of its manifestations and recommendations in respect to its early diagnosis and surgical tactics.

[Laparoscopic microcholecystostomy and its complications].

An original method of laparoscopic microcholecystostomy is proposed based on using a spiral catheter and 3 variants of performing this operation. Causes of complications are analyzed. The method of laparoscopic microcholecystostomy used in 426 patients enabled the authors to reduce the incidence of complications from 11.

[Study of the structural changes in irradiated erythrocyte membranes using 2,6-toluidinonaphthalene sulfonate].

It was shown that 2,6-tolyidinonaphthalene sulfonate (2,6-TNS) is localized mainly at the bilayer-water border of the erythrocytic membranes. Under the effect of gamma-radiation the rearrangements occur in the membrane which bring about changes in the distribution of the probe between the membrane and the medium. The lifetime of the excited state of 2,6-TNS after irradiation varies slightly.

[Study of the aggregation of membrane proteins of erythrocyte ghosts using SDS-PAAG electrophoresis].

Using the method of electrophoresis in SDS-PAAG the authors showed a diminution of proteins of bands I + II (spectrins) and III (major integral protein) after irradiation of erythrocyte ghosts with doses of 50 to 1000 Gy. We failed to ascertain that radiation-induced lipid peroxidation is involved into membrane protein aggregation. Among the radiolysis products, OH-radicals were shown to contribute markedly to the radiation effect observed.

The effect of ionizing radiation on tryptophan fluorescence of thymocyte and erythrocyte plasma membranes.

The effect of ionizing radiation on tryptophan fluorescence of thymocyte and erythrocyte plasma membrane preparations was studied. The intensity of tryptophan fluorescence decreased after applying radiation doses up to 15 Gy. The radiosensitivity of thymocyte membranes appeared to be higher than that of the erythrocyte ghosts.

Use of trinitrobenzenesulfonic acid in the determination of protein amino groups on the external surface of rat erythrocyte membranes.

Description is provided of conditions selected for the study of protein amino groups (AG) on the external surface of rat erythrocyte membranes (EM) by means of 2,4,6-trinitrobenzenesulfonic acid (TNBS). The number of protein AG reacting with TNBS was influenced by the season of the year, the physiologic state of the donor animal, and other factors known to alter EM. It appears that structural perturbations introduced by these factors into EM are responsible for alterations in the accessibility of the protein AG to TNBS.