Epithelial apical glycosylation changes associated with thin endometrium in women with infertility - a pilot observational study.

Background: Low endometrial receptivity is one of the major factors affecting successful implantation in assisted reproductive technologies (ART). Infertile patients with thin endometrium have a significantly lower cumulative clinical pregnancy rate than patients with normal endometrium. Molecular pathophysiology of low receptivity of thin endometrium remains understudied.

Perturbed Ca-dependent signaling of DYT2 hippocalcin mutant as mechanism of autosomal recessive dystonia.

A recent report of autosomal-recessive primary isolated dystonia (DYT2 dystonia) identified mutations in HPCA, a gene encoding a neuronal calcium sensor protein, hippocalcin (HPCA), as the cause of this disease. However, how mutant HPCA leads to neuronal dysfunction remains unknown. Using a multidisciplinary approach, we demonstrated the failure of dystonic N75K HPCA mutant to decode short bursts of action potentials and theta rhythms in hippocampal neurons by its Ca-dependent translocation to the plasma membrane.

Measurement of intracellular concentration of fluorescently-labeled targets in living cells.

Estimations of intracellular concentrations of fluorescently-labeled molecules within living cells are very important for guidance of biological experiments and interpretation of their results. Here we propose a simple and universal approach for such estimations. The approach is based upon common knowledge that the dye fluorescence is directly proportional to its quantum yield and the number of its molecules and that a coefficient of proportionality is determined by spectral properties of the dye and optical equipment used to record fluorescent signals.

[Superior vena cava blood flow measurements for results prognosing of a bidirectional cava-pulmonary anastomosis in correction of hemodynamics parameters in patients with hypoplastic right ventricle].

The method of prediction the enability of closure atrial septal defect before operation or in pre-bypass period during the one and one-half ventricle repair of CHD with hypoplastic right ventricle was presented. The method is based on comparison of the initial blood flow on superior vena cava (SVC) and right-to-left shunt at atrial level. Defect can be closed if flow on SVC and shunt flow are approximately equal.

Decoding glutamate receptor activation by the Ca2+ sensor protein hippocalcin in rat hippocampal neurons.

Hippocalcin is a Ca(2+)-binding protein that belongs to a family of neuronal Ca(2+)sensors and is a key mediator of many cellular functions including synaptic plasticity and learning. However, the molecular mechanisms involved in hippocalcin signalling remain illusive. Here we studied whether glutamate receptor activation induced by locally applied or synaptically released glutamate can be decoded by hippocalcin translocation.

Hippocalcin signaling via site-specific translocation in hippocampal neurons.

Hippocalcin is a Ca(2+)-binding protein, which belongs to the family of neuronal Ca(2+) sensors. It is highly expressed in the hippocampus but molecular mechanisms underlying its action in this part of the brain have not been investigated in detail. To study whether intrinsic neuronal activity could result in hippocalcin-mediated signal transduction we examined spontaneous and action potential (AP)-dependent changes in fluorescence of yellow fluorescent protein-tagged hippocalcin (HPCA-YFP) in transiently transfected hippocampal cultured neurons.

Pseudoaneurysm of subclavian artery 21-years after staged repair of tetralogy of Fallot.

Three cases of pseudoaneurysm of subclavian artery in long-term follow-up after staged repair of tetralogy of Fallot are described. The angiographical study revealed that aneurysms were located in the systemic end area of previously ligated modified Blalock-Taussing shunt. Aneurysmectomy with resection of a shunt and segment of subclavian artery was performed.

[The conduit application in surgical treatment of corrected transposition of the main vessels].

Experience of surgical treatment of 45 patients with corrected transposition of the main vessels (CTMV) is summarized. Indications and tactic of surgical intervention for CTMV with the pulmonary trunk (PT) stenosis using conduit as a parallel exit from venous ventricle are substantiated. Original construction of autopericardial conduit is adduced.

[Complete cava-pulmonary conjunction in surgical treatment of heart abnormalities with a single functionally active ventricle].

Complete cavapulmonary conjunction (CCPC) was done in 27 patients, in 3 of whom the tricuspid valve atresia and in 24--various forms of the heart only ventricle. In the early postoperative period 7 (25.9%) patients died.

[The basic anatomical variants of the single heart ventricle and the characteristics of their clinical courses].

Results are analysed of examination of 146 patients with different anatomical variants of single ventricle of the heart. 52 cases (35.6%) demonstrated biflow left ventricle, 49 (33.

[The use of bi-directional cava-pulmonary anastomosis in the treatment of severe congenital heart defects].

Two-directional cava-pulmonary anastomosis was done in 35 patients with a inborn heart failure, ageing from 10 months to 11 years. In 29 patients the significant improvement of their state was noted. The level of arterial blood saturation with an oxygen have rised up to (90.

[Plasminogen activators in assessing the reaction of patients with chronic obstructive bronchitis to physical loading].

Patients with chronic obstructive bronchitis showed an increased activity of the blood plasminogen activator in a group of patients under 40 years of age and absence of the dynamics of this index an older age group. The urokinase urinary activity did not change in the two groups. The blood activator activity increased in healthy young non-trained and trained young persons under the effect of loads.

[The plasma fibronectin content and its significance in patients with bronchial asthma].

Reduction of the content of fibronectin in blood plasma depending on severity and form of the disease was revealed in patients with infection-dependent bronchial asthma. Discreteness of influence of autologous leucocytes upon the concentration of fibronectin in plasma, caused by the factor of hormone dependence of the disease was discovered. It was determined that tactivin recovers capability of leucocytes in the patients with hormone dependent asthma directly (monocytes) or indirectly (Lymphocytes, neutrophils) to produce fibronectin.