Morphometric analysis of sperm used for IVP by three different separation methods with spatial light interference microscopy.

The goal of this study was to characterize sperm populations resulting from three different methods of sperm selection used for bovine fertilization. We compared sperm selection with discontinuous Percoll gradients, Swim-Up, and electro-channel. Spatial light interference microscopy (SLIM) was used to evaluate the morphology of the spermatozoa and computer-assisted semen analysis (CASA) was used to evaluate the motility behavior of the sperm.