Analysis of free and total myo-inositol in foods, feeds, and infant formula by high-performance anion exchange chromatography with pulsed amperometric detection, including a novel total extraction using microwave-assisted acid hydrolysis and enzymatic treatment.

A method for the analysis of free and total myo-inositol in foods, feeds, and infant formulas has been developed and validated using high-performance anion exchange chromatography with pulsed amperometric detection. The option of a free myo-inositol determination or a complete total myo-inositol determination from main bound sources can be achieved. These sources include phytates, lower'phosphorylated forms, and phosphatidylinositol.

Application of ultra-high-performance liquid chromatography/tandem mass spectrometry for the measurement of vitamin D in infant formula and adult/pediatric nutritional formula: First Action 2011.11.

In an effort to measure vitamin D, ultra-high-performance liquid chromatography with tandem mass spectrometry (UHPLC-MS/MS) was applied to samples. The use of UHPLC-MS/MS decreased the run time by 50%. The UHPLC-MS/MS achieved equal or better separation efficiency with complex food matrixes compared to HPLC-MS/MS.

Application of ultra-performance liquid chromatography/tandem mass spectrometry for the measurement of vitamin D in foods and nutritional supplements.

Ultra-performance liquid chromatography (UPLC)/MS/MS was applied to measure vitamin D in various foods and nutritional supplements. The run-time of the chromatographic separation was cut from 20 min in HPLC/MS/MS to 10 min in UPLC/MS/MS, while equal or better separation efficiency was achieved to deal with complex food matrixes. Under the optimized conditions, all the previtamins of vitamin D3, D2, and isotope-labeled vitamin D3 were baseline-separated from their corresponding vitamins.

Measurement of water-soluble B vitamins in infant formula by liquid chromatography/tandem mass spectrometry.

A method has been developed for the simultaneous measurement of multiple B vitamins (i.e., B1, B2, B3, B5, and B6) in infant formulas by LC-MSIMS.

Measurement of vitamin D in foods and nutritional supplements by liquid chromatography/tandem mass spectrometry.

Vitamin D is a fat-soluble vitamin with great nutritional interest. An HPLC/MS/MS method was developed to measure vitamin D with atmospheric pressure chemical ionization. Under the experimental parameters used, the LOQ was 0.

Proposed modifications to AOAC 996.06, optimizing the determination of trans fatty acids: presentation of data.

The increased focus on the accuracy of trans fatty acid data generated using current methodologies has resulted in research initiatives to optimize the quality of these assays. In this study, scientists combined the established methodology from AOAC 996.06 and the American Oil Chemists Society method Ce 1h-05, as well as other independent research.