Gelatin capsules for post mortem vitrification of Piracanjuba (Brycon orbignyanus) ovarian tissue as an accessible solution for emergency genetic preservation.

Environmental disasters have caused significant losses in fish populations, threatening the genetic diversity of many species. Cryopreservation of genetic material post-mortem has emerged as a potential solution to safeguard biodiversity. However, a fast, accessible method for cryopreserving such material in emergencies is needed.

Short-Term Storage of Collared Peccary Semen in a Transport Container at 5°C Using Different Extenders.

The advancement of short-term storage methods for collared peccary semen targets its potential application in artificial insemination programmes and for combination with cryopreservation techniques. The objective of this study was to evaluate the performance of a transport container (Botutainer) for the preservation of collared peccary semen using commercial extenders (BTS, NUTRIXcell+ and PRIMXcell Ultra) as well as a TRIS + egg yolk extender. Ten ejaculates obtained by electroejaculation were diluted and stored at 5°C for 72 h.

Tropical forests in the Americas are changing too slowly to track climate change.

Understanding the capacity of forests to adapt to climate change is of pivotal importance for conservation science, yet this is still widely unknown. This knowledge gap is particularly acute in high-biodiversity tropical forests. Here, we examined how tropical forests of the Americas have shifted community trait composition in recent decades as a response to changes in climate.

A New Instrument to Measure Individual Finger Strength in Palmar Grip.

Functional assessment of the upper limbs is the most effective method to verify the motor condition. However, not all dynamometers are sensitive enough to measure individual finger contribution to the force. This paper focuses on a novel virtual instrumentation developed to measure palm grip strength.

Oxidation-responsive, settable bone substitute composites for regenerating critically-sized bone defects.

Critically-sized bone defects that cannot spontaneously heal on their own remain a significant problem in the clinic. Synthetic polymeric implants are promising therapies for improving bone healing as they are highly tunable and avoid the potential complications associated with autologous bone grafts. However, biostable implants such as poly(methyl methacrylate) (PMMA) suffer from numerous shortcomings including negligible biodegradability and limited osseointegration with bone.

Development, Safety, and Therapeutic Evaluation of Voriconazole-Loaded Zein-Pectin-Hyaluronic Acid Nanoparticles Using Alternative In Vivo Models for Efficacy and Toxicity.

: Fungal infections caused by species remain a significant clinical challenge, exacerbated by limitations in current antifungal therapies, including toxicity and poor bioavailability. This study aimed to develop and evaluate voriconazole-loaded zein-pectin-hyaluronic acid nanoparticles (ZPHA-VRC NPs) as a novel drug delivery system to enhance efficacy and reduce toxicity. Alternative in vitro and in vivo models were utilized to assess the safety and therapeutic potential of the nanoparticles.

Brazilian Stingless Bee Geopropolis Exhibit Antioxidant Properties and Anticancer Potential Against Hepatocellular Carcinoma Cells.

Hepatocellular carcinoma (HCC) is the third most common cancer in terms of mortality and the sixth in incidence worldwide. Treatment varies by tumor stage, but low survival rates are common across all stages. Due to these poor outcomes, there is a critical need for new treatment options and lead compounds, prompting an active search.

Unraveling the role of satellite DNAs in the evolution of the giant XY sex chromosomes of the flea beetle Omophoita octoguttata (Coleoptera, Chrysomelidae).

Background: The flea beetle Omophoita octoguttata (Coleoptera, Chrysomelidae) is a member of a group in which the males completely lack meiotic recombination (male-specific achiasmy) and that have extraordinarily large X and Y chromosomes. We combined genome sequencing, including microdissected Y and X chromosomes, and cytogenetic in situ hybridization studies, to evaluate the potential role of satellite DNAs (satDNAs) in the differentiation of those gigantic sex chromosomes.

Results: We report flow cytometry results showing that this species has a very large genome size (estimated to be 4.

Use of Digital Health Technologies for Dementia Care: Bibliometric Analysis and Report.

Background: Dementia is a syndrome that compromises neurocognitive functions of the individual and that is affecting 55 million individuals globally, as well as global health care systems, national economic systems, and family members.

Objective: This study aimed to determine the status quo of scientific production on use of digital health technologies (DHTs) to support (older) people living with dementia, their families, and care partners. In addition, our study aimed to map the current landscape of global research initiatives on DHTs on the prevention, diagnosis, treatment, and support of people living with dementia and their caregivers.

Antiviral Activity of Electrospun Polyamide Ultrathin Fibers Against SARS-CoV-2 Variant.

The development of new strategies to produce nanomaterials that can be used as personal protective equipment with antiviral activity and low toxicity is crucial. Electrospun ultrathin fibers have attracted considerable attention due to their potential for biomedical applications, including antiviral activity. Herein, we electrospun different grades of commercially available polyamide to produce ultrathin fibers and investigate their antiviral activity against SARS-CoV-2 Gamma lineage (P.

Unraveling the differences between pyrolytic and petrogenic sources in oiled areas of the South Atlantic: An analytical method for biliary PAHs.

Polycyclic aromatic hydrocarbons (PAHs) represent significant organic pollutants within the scope of environmental monitoring and management investigations of oiled areas. Characterized as precursors of mutagenic and carcinogenic processes, they exert a direct influence on human and environmental health. To assess the impacts of one of the largest oil spills affecting coral reefs in Alagoas, Brazil, researchers developed, validated, and applied an innovative methodology.

Comparing apples with apples: A proposed taxonomy for "Community Health Workers" and other front-line health workers for international comparisons.

This paper proposes a taxonomy for Community Health Workers (CHWs) and others engaged in front-line community health activities, encompassing formally-employed workers extending government primary health care (PHC) service delivery as well as a range of other actors with roles at the nexus of government PHC and communities. The taxonomy is grounded in current definitions from the World Health Organization and the International Labor Organization, and proposes some refinements for future iterations of guidance from these agencies. The designation, "Community Health Worker" is currently used to cover a broad range of roles.

Developing robust food composition models: Strategies for handling temperature and packaging variations in dry-cured ham using near infrared spectrometry.

Low-cost near infrared devices intended for consumers able to easily determine composition and quality of food products may boost adoption of sustainable healthy diets. However, predictive algorithms robust to external variations are needed. The aim of this work was to evaluate different data analysis strategies to develop robust predictive models for food composition when using spectrometric data subjected to external variations, specifically temperature and packaging material, acquired using low-cost sensors.

IgM against Merozoite Surface Protein 1-Block 2 Haplotypes as New Tools for Infections.

Introduction: The tools to distinguish relapse from reinfection are needed in malaria-endemic areas. We evaluated seroprevalence against sets of specific peptides to the block 2 region of -merozoite surface protein-1 (PvMSP1) to detect parasite clones.

Methods: We applied amplicon deep sequencing (ADS) of block 2 region of the MSP-1 gene () to determine cocirculating parasite clones within eight -infected individuals.

Evolutionary dynamics of the B chromosomes in the fish species Valenciennes, 1837 of the Paraná River Basin.

The fish species has an interesting B chromosome system, with three morphological types as acrocentric, metacentric, and submetacentric. However, most cytogenetic studies on this species are restricted to the natural population of the Mogi Guaçu River. Given this, the present work aimed to study the structure karyotypic profile as well as the occurrence of supernumeraries in in several localities in the Paraná River basin, where this species is abundant.

Computational Methods for Modeling Lipid-Mediated Active Pharmaceutical Ingredient Delivery.

Lipid-mediated delivery of active pharmaceutical ingredients (API) opened new possibilities in advanced therapies. By encapsulating an API into a lipid nanocarrier (LNC), one can safely deliver APIs not soluble in water, those with otherwise strong adverse effects, or very fragile ones such as nucleic acids. However, for the rational design of LNCs, a detailed understanding of the composition-structure-function relationships is missing.

Chemical Characterization and Antimicrobial Activity of Essential Oils and Nanoemulsions of and .

This study aimed to develop gel nanoemulsions (NEs) of Brazilian essential oils (EOs) from and , as well as to perform chemical characterization and investigate the antimicrobial activity of the EOs and NEs. : The main chemical compounds of EO were curzerene (34.80%) and germacrene B (11.

Bcl-2 and Bcl-xL in Diabetes: Contributions to Endocrine Pancreas Viability and Function.

Diabetes is a chronic metabolic disorder whose prevalence increases every year, affecting more than 530 million adults worldwide. Type 1 (T1D) and type 2 diabetes (T2D), the most common forms of diabetes, are characterized by the loss of functional pancreatic β-cells, mostly due to apoptosis. B-cell leukemia/lymphoma 2 (Bcl-2) and B-cell lymphoma-extra large (Bcl-xL), two anti-apoptotic proteins belonging to the Bcl-2 family, are crucial for regulating the intrinsic pathway of apoptosis.

Early ultrastructural damage in retina and optic nerve following intraocular pressure elevation.

Elevated intraocular pressure (IOP) is a significant risk factor for glaucoma, causing structural and functional damage to the eye. Increased IOP compromises the metabolic and structural integrity of retinal ganglion cell (RGC) axons, leading to progressive degeneration and influencing the ocular immune response. This study investigated early cellular and molecular changes in the retina and optic nerve (ON) following ocular hypertension (OHT).

Intranasal liposomal remdesivir induces SARS-CoV-2 clearance in K18-hACE2 mice and ensures survival.

A huge challenge after the emergence of COVID-19 has been the discovery of effective antiviral drugs. Although remdesivir (RDV) emerged as one of the most promising drugs, its pharmaceutical formulation Veklury® is limited by moderate efficacy, high toxicity and need for parenteral administration. The aim of the present work was to develop a liposomal formulation of RDV for pulmonary administration and evaluate its efficacy in models of COVID-19.

Rhamnogalacturonan promotes intestinal mucosal repair through increased cell migration.

Mucosal healing is the primary goal for inflammatory bowel disease (IBD) treatment. We previously showed the direct beneficial effects of rhamnogalacturonan (RGal) on intestinal epithelial barrier function. Here, we aimed to evaluate the effect of RGal in intestinal epithelial wound healing.

Examining the under-representation of black researchers in sports science and sports medicine publications: 'You Can't be what You Can't See'.

Objectives: This study evaluated the representation of black researchers as authors of articles published in four peer-reviewed journals with the highest impact factors (IFs) in the field of sports science and sports medicine.

Methods: An analysis was conducted on articles published between 2018 and 2022 in four leading sports science journals with high IFs (2022): The British Journal of Sports Medicine, Journal of Sport and Health Science, Sports Medicine and Exercise Immunology Review. Data extraction from the articles included the researcher's names, sex/gender, total number of authors, number of black authors, their position in the author list, publication year, article title and type and digital object identifier.

Twelve weeks of exercise training improves cognitive status, physical performance and quality of life in Alzheimer's disease: A systematic review and meta-analysis.

Alzheimer's disease (AD) is a progressive neurodegenerative disorder in which there is slow and gradual impairment of mental function. Considering the increase in cases due to population aging, the potential benefits of physical training in AD are of great importance and need further elucidation. This study aims to identify the impact of physical training on crucial aspects of AD such as cognitive status, physical performances, quality of life and activities of daily living.

Characterization of Three Novel Papillomavirus Genomes in Vampire Bats ().

Bats are mammals with high biodiversity and wide geographical range. In Brazil, three haematophagous bat species are found. is the most documented due to its role as a primary host of rabies virus in Latin America.