Molecular diversity of phototrophic biofilms on building stone.

Composition and diversity of aeroterrestrial phototrophic microbial communities are up to now poorly understood. Here, we present a comparative study addressing the composition of algal communities on sandstone substrata based upon the analysis of rRNA gene clone libraries from environmental samples and crude cultures. From a west-facing, shaded wall area of the mediaeval castle ruin Gleichen (Thuringia, Germany), sequences mainly related to the green algae Prasiococcus and Trebouxia (Trebouxiophyceae) were retrieved.