Publications by authors named "Dorota Sklenarova"
Article Synopsis
- Laser-induced breakdown spectroscopy (LIBS) enhances immunochemical assays using indirect label detection with nanoparticles, specifically yttrium-based photon-upconversion nanoparticles (UCNPs), achieving sensitivity comparable to established methods like fluorescence immunoassays.
- The study improves the sensitivity of UCNP-based Tag-LIBS by incorporating magnetic microbeads (MBs), which allow for analyte preconcentration and significantly lowers the limit of detection (LOD) to 4.0 pg/mL, a notable advancement over traditional microtiter plate methods.
- The technique demonstrates robust performance in clinical samples and shows strong correlation with standard assays, indicating that MB-based Tag-LIBS offers fast readout, high sensitivity, and potential for multiplexing without relying
Article Synopsis
- Early diagnosis of prostatic carcinoma is crucial for effective treatment and better patient outcomes, and the standard method involves detecting prostate-specific antigen (PSA) non-invasively.
- A new microfluidic device has been created that enhances the sensitivity of PSA detection using photon-upconversion nanoparticles (UCNPs) and optimized magnetic microparticles, achieving a remarkable detection limit of 1.04 pg/mL.
- This microfluidic technique not only simplifies the testing process but also allows for high-throughput analysis and has shown reliable results when tested against standard reference methods in clinical samples.
Conventional immunochemical methods used in clinical analysis are often not sensitive enough for early-stage diagnosis, resulting in the need for novel assay formats. Here, we provide a detailed comparison of the effect of different labels and solid supports on the performance of heterogeneous immunoassays. When comparing three types of streptavidin-modified labels─horseradish peroxidase, carboxyfluorescein, and photon-upconversion nanoparticles (UCNPs)─UCNPs led to the most sensitive and robust detection of the cancer biomarker prostate-specific antigen.
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