Synthesis and characterization of imprinted sorbent for separation of gramine from bovine serum albumin.

The aim of this study was to develop an efficient sorbent for separation of N,N-dimethyl-3-aminomethylindole (gramine) from bovine serum albumin. An imprinting technology was involved in the synthesis of polymers from nine different functional monomers in the presence of ethylene glycol dimethacrylate as a cross-linker. The analysis of binding capacities showed that the highest specificity towards gramine was achieved when 4-vinylbenzoic acid was used as the functional monomer in methanol to form the bulk imprinted polymer, MIP1 (imprinting factor equal to 21.

Desorption of 3,3'-diindolylmethane from imprinted particles: An impact of cross-linker structure on binding capacity and selectivity.

Here, seven cross-linkers (six polar diacrylates or dimethacrylates of different lengths between double bonds, and one aromatic-divinylbenzene) were used to examine the impact of the cross-linker on binding capacity and selectivity of 3,3'-diindolylmethane (DIM) imprinted material. DIM participates in the suppression of viability of human ovarian and human breast cancer cell lines, but has low bioavailability. The investigations of novel imprinted polymer matrices for improvement of DIM release could allow to utilize not only a potency of DIM but also similar alkaloids, which are the important compounds with pharmacological activity.

[Investigations of molecularly imprinted polymers for medical diagnostics and clinical analysis].

The review focuses on progress of molecularly imprinted polymers for medical diagnostics and clinical analysis. This class of new and selective polymeric materials could find future applications in monitoring of diabetes and tumors, in diagnostics of kidney and heart diseases, arteriosclerosis or in blood analysis.