Metagenomic insights into the wastewater resistome before and after purification at large‑scale wastewater treatment plants in the Moscow city.

Wastewater treatment plants (WWTPs) are considered to be hotspots for the spread of antibiotic resistance genes (ARGs). We performed a metagenomic analysis of the raw wastewater, activated sludge and treated wastewater from two large WWTPs responsible for the treatment of urban wastewater in Moscow, Russia. In untreated wastewater, several hundred ARGs that could confer resistance to most commonly used classes of antibiotics were found.

New insight into the granule formation in the reactor for enhanced biological phosphorus removal.

While granulated activated sludge exhibits high productivity, the processes of granule formation are incompletely studied. The processes of granule formation and succession of communities were investigated in a laboratory sequencing batch reactor (SBR) under conditions for enhanced biological phosphorus removal (EBPR) using microbiological and molecular techniques. Active consumption of acetate, primarily by the phosphate-accumulating organisms (PAO), commenced at day 150 of cultivation.

[Assessment of exo- and endocrine function of pancreas following distal pancreatectomy].

Aim: The assessment of pancreatic resection volume influence on exo- and endocrine pancreatic functions.

Materials And Methods: The resected pancreatic volume influence was assessed in 47 patients: 31 (66%) patients after resections of pancreatic body and tail, and 16 (34%) patients after distal resections. The exocrine pancreatic function was assessed by pancreatic fecal elastase 1 as well as endocrine pancreatic function was assessed by C-peptide level measurement.

Diagnostic value of screening methods for the determination of antinuclear antibodies using indirect immunofluorescence on HEp-2 cells and enzyme immunoassay in autoimmune liver diseases.

Antinuclear antibodies (ANA) are a heterogeneous group of autoantibodies that react with various components of the cell nucleus and cytoplasm. ANA is the main serological marker for autoimmune liver disease (AILD). The aim of the study was to compare the diagnostic value of two methods of screening for the determination of ANA (indirect immunofluorescence reaction on HEp-2 cells (IIF -HEp-2) and enzyme-linked immunosorbent assay (ELISA) in the sera of AILD patients.

Previous hepatitis B viral infection-an underestimated cause of pancreatic cancer.

Background: The etiology of pancreatic cancer remains unclear. This limits the possibility of prevention and effective treatment. Hepatitis B virus (HBV) is responsible for the development of different types of cancer, but its role in pancreatic cancer is still being discussed.

The structure of microbial communities of activated sludge of large-scale wastewater treatment plants in the city of Moscow.

Microbial communities in wastewater treatment plants (WWTPs) play a key role in water purification. Microbial communities of activated sludge (AS) vary extensively based on plant operating technology, influent characteristics and WWTP capacity. In this study we performed 16S rRNA gene profiling of AS at nine large-scale WWTPs responsible for the treatment of municipal sewage from the city of Moscow, Russia.

New Insight Into the Interspecies Shift of Anammox Bacteria "Brocadia" and "Jettenia" in Reactors Fed With Formate and Folate.

The sensitivity of anaerobic ammonium-oxidizing (anammox) bacteria to environmental fluctuations is a frequent cause of reactor malfunctions. It was hypothesized that the addition of formate and folate would have a stimulating effect on anammox bacteria, which in turn would lead to the stability of the anammox process under conditions of a sharp increase in ammonium load, i.e.

[The characteristics of medical demographic indices in the Jewish autonomous oblast].

The research in the field of regional trends and risk factors related to population morbidity is considered as background of management decision-making in health care. The article presents the results of analysis of medical demographic indices and population health status in the Jewish Autonomous Oblast in 2000-2019. It is established that in in the Jewish Autonomous Oblast population size reduced by 35 100 people to 2019.

Prevalence and Structure of Periodontal Disease and Oral Cavity Condition in Patients with Coronary Heart Disease (Prospective Cohort Study).

Purpose: To study the incidence and structure of periodontal disease in elderly Moscow residents suffering from permanent coronary heart disease, as well as examine the oral cavity and tooth structure in patients with generalized periodontitis and coronary heart disease.

Patients And Methods: Stage 1 (studying the incidence and structure of periodontal diseases) enrolled 330 patients over 50 years old: Group 1 consisted of 180 patients (102 males and 78 females) with stable coronary heart disease; Group 2 consisted of 150 dental patients (90 males and 60 females) with periodontal pathology without associated coronary heart disease. Stage 2 enrolled 216 patients with generalized periodontitis (studying features of the generalized periodontitis course depending on the coronary heart disease presence): Group 1 consisted of 145 patients with coronary heart disease and generalized periodontitis (79 males and 66 females), Group 2 consisted of 71 patients with generalized periodontitis but without coronary heart disease (40 males and 31 females).

May Previous Hepatitis B Virus Infection Be Involved in Etiology and Pathogenesis of Autoimmune Liver Diseases?

Introduction: Viral infections, especially with hepatotropic viruses, may trigger autoimmune liver diseases (AILDs) and deteriorate their course. However, association of previous hepatitis B virus (HBV) infection (presence of anti-HBc with or without anti-HBs or HBV DNA in serum) with AILDs is poorly studied so far. The aim of the study was to assess the prevalence of previous hepatitis B virus infection markers and its clinical significance in patients with autoimmune liver diseases.

Investigation of Mechanical and Microstructural Properties of Welded Specimens of AA6061-T6 Alloy with Friction Stir Welding and Parallel-Friction Stir Welding Methods.

The present study investigates the effect of two parameters of process type and tool offset on tensile, microhardness, and microstructure properties of AA6061-T6 aluminum alloy joints. Three methods of Friction Stir Welding (FSW), Advancing Parallel-Friction Stir Welding (AP-FSW), and Retreating Parallel-Friction Stir Welding (RP-FSW) were used. In addition, four modes of 0.

Novel CAR T therapy is a ray of hope in the treatment of seriously ill AML patients.

Acute myeloid leukemia (AML) is a serious, life-threatening, and hardly curable hematological malignancy that affects the myeloid cell progenies and challenges patients of all ages but mostly occurs in adults. Although several therapies are available including chemotherapy, allogeneic hematopoietic stem cell transplantation (alloHSCT), and receptor-antagonist drugs, the 5-year survival of patients is quietly disappointing, less than 30%. alloHSCT is the major curative approach for AML with promising results but the treatment has severe adverse effects such as graft-versus-host disease (GVHD).

Preparation of W-C-Co Composite Micropowder with Spherical Shaped Particles Using Plasma Technologies.

The possibility of obtaining composite micropowders of the W-C-Co system with a spherical particle shape having a submicron/nanoscale internal structure was experimentally confirmed. In the course of work carried out, W-C-Co system nanopowders with the average particle size of approximately 50 nm were produced by plasma-chemical synthesis. This method resulted in the uniform distribution of W, Co and C among the nanoparticles of the powder in the nanometer scale range.

Nanoscale real-time detection of quantum vortices at millikelvin temperatures.

Since we still lack a theory of classical turbulence, attention has focused on the conceptually simpler turbulence in quantum fluids. Reaching a better understanding of the quantum case may provide additional insight into the classical counterpart. That said, we have hitherto lacked detectors capable of the real-time, non-invasive probing of the wide range of length scales involved in quantum turbulence.

Renaissance of armored immune effector cells, CAR-NK cells, brings the higher hope for successful cancer therapy.

In recent decades, a new method of cellular immunotherapy was introduced based on engineering and empowering the immune effector cells. In this type of immunotherapy, the immune effector cells are equipped with chimeric antigen receptor (CAR) to specifically target cancer cells. In much of the trials and experiments, CAR-modified T cell immunotherapy has achieved very promising therapeutic results in the treatment of some types of cancers and infectious diseases.

A standardised model for stool banking for faecal microbiota transplantation: a consensus report from a multidisciplinary UEG working group.

Background: Faecal microbiota transplantation is an emerging therapeutic option, particularly for the treatment of recurrent Clostridioides difficile infection. Stool banks that organise recruitment and screening of faeces donors are being embedded within the regulatory frameworks described in the European Union Tissue and Cells Directive and the technical guide to the quality and safety of tissue and cells for human application, published by the European Council.

Objective: Several European and international consensus statements concerning faecal microbiota transplantation have been issued.

[Сlinical, immunological and morphological features in different types of autoimmune hepatitis].

Unlabelled: Due to the absence of the pathognomonic diagnostic criteria and to the diversity of clinical, serological and morphological manifestations, the diagnostic of the autoimmune hepatitis (AIH) remains to be a difficult task, which might lead to the delay of the timely beginning of the immunosuppressive therapy (IST), which in turn affects the disease outcomes.

Aim: To studying the clinical, biochemical, immunological and morphological markers in patients with seronegative (SN) and seropositive (SP) AIH and the qualities of their response to the IST.

Materials And Methods: This retrospective cohort study included 82 AIH patients over the course of the years 20142019.

Dental anomalies in people living in radionuclide-contaminated regions.

The 1986 accident at the Chernobyl Nuclear Power Plant led to large-scale changes in the environmental situation. The purpose of our study was to conduct a comparative analysis of the morphological states of the dentition of individuals living in regions exposed to radiation to determine the groups at risk for the main classes of dental anomalies. We believe our results will support the development of a differentiated system for dental rehabilitation and follow-up of individuals exposed to radiation.

A STUDY OF THE PREVALENCE OF NON-CARIOUS DENTAL LESIONS RELATED TO PRODUCTION FACTORS IN RESIDENTS OF THE CITY OF PENZA.

The aim of the study was to study the current problems of non-carious dental lesions in residents of Penza, depending on the influence of production factors. In the structure of non-carious lesions, there is a large group of diseases (and injuries) of the hard tissues of the teeth (enamel, dentin, cement), which have their various clinical manifestations, the occurrence and development of which is not directly related to the microbial factor, unlike carious lesions. Such diseases are enamel and dentin hypoplasia, fluorosis, wedge-shaped defect, etc.

Opposite hinge fractures in high tibial osteotomy: a displacement subtype is more critical than a fracture type.

Purpose: Analysis of the structure of the fractures of opposite hinge (FOH) after angle-stable closed-wedge (CW) and open-wedge (OW) high tibial osteotomy (HTO), and their influence on the development of tibial pseudarthrosis.

Methods: 187 CW and 94 OWHTOs were analyzed retrospectively. The FOHs in the OWHTO were classified according to Takeuchi, and in the CWHTO-according to the own classification with two types (depending on the direction of FOH).

Opposite cortical fractures in closed-wedge HTO: New classification and treatment algorithm.

Background: An intact opposite cortex (OC) is essential for HTO stability. For an appropriate prognosis of the role of opposite cortical fracture (OCF) in the development of mechanical complications, it is important to identify the type of OCF. This study seeks to establish an OCF classification in CWHTO with a treatment algorithm.

[Complication analysis after angle-stable CW and OW high tibial osteotomy].

Background: High tibial osteotomy (HTO) is a good joint-preserving alternative to joint replacement in the treatment of isolated medial varus gonarthrosis. It is, however, accompanied by a number of complications, which can compromise the outcome of the treatment.

Objectives: Analysis and comparison of the complication structure after angle-stable navigated closed wedge (CW) HTO and conventional angle-stable open wedge (OW) HTO, as well as determination of influence factors.

[The experimental clinical substantiation of treatment of patients with odontogenic phlegmon of maxillofacial area using Dаlargin in complex therapy.].

The patients with odontogenic phlegmon of maxillofacial area suffer from immune depression at the surgical stage of treatment that can unfavorably affect their rehabilitation. The study analyzes possibility of application of medication Dalargin in complex treatment of patients during post-operation period. The experimental study established components of immune system affected by medication.

[The characteristics of crystallization of mixed saliva in patients using heroin and methadone.].

According many publications, rate and severity of various diseases of oral cavity are significantly increasing in drug-dependent patients. One of drugs using in many countries as a substitution therapy is methadone. However, last research data testifies that negative effect of methadone on human organism in general is more expressed as compared with case of heroin.