Publications by authors named "Doris Rosenfellner"
Objective: To evaluate the impact of dynamic in vitro culture on initiation of early follicular growth in prepubertal mouse ovaries.
Design: Ovaries from 8-day-old BALB/c mice were cultured either in a dynamic system (n=28) or in a static system (n=20) for 4 days. Uncultured 8-day-old (n=9) or 12-day-old (n=17) ovaries served as baseline or in vivo controls, respectively.
Article Synopsis
- The use of replication-deficient retroviral vectors for gene therapy in solid tumors has limitations in efficiency, prompting exploration of replication-competent retroviral vectors as an alternative.
- Research in this study examined how different design features of these vectors affect their spread, genomic stability, and expression levels in both murine and human cell cultures and in tumors in mice.
- Findings indicate that Moloney murine leukemia virus (Mo-MLV)-based vectors perform better than Akv-MLV-based vectors, with faster spread, higher expression, and more genomic stability; the position of the transgene cassette also influences stability, and the effects vary based on the cell line used.
To facilitate a more efficient radiation and chemotherapy of mammary tumours, synthetic enhancer elements responsive to hypoxia and ionizing radiation were coupled to the mammary-specific minimal promoter of the murine whey acidic protein (WAP) encoding gene. The modified WAP promoter was introduced into a retroviral promoter conversion (ProCon) vector. Expression of a transduced reporter gene in response to hypoxia and radiation was analysed in stably infected mammary cancer cell lines and an up to 9-fold increase in gene expression demonstrated in comparison to the respective basic vector.
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