Publications by authors named "Doris Joy D Espiritu"
Role of NH(2) and COOH termini in targeting, stability, and activity of sodium bicarbonate cotransporter 1.
Am J Physiol Renal Physiol
September 2006
Article Synopsis
- Sodium bicarbonate cotransporter 1 (NBC1) is crucial for bicarbonate reabsorption in kidneys, but the mechanisms influencing its function and stability are unclear.
- Researchers tested truncation mutants of NBC1, specifically DeltaN424 and DeltaC92, and found that while they reached the membrane, they did not exhibit NBC1 activity and DeltaC92 was more prone to endocytosis.
- The stability of NBC1 at the basolateral membrane involves its COOH terminus, and cotransfecting both mutants together helped restore some functional activity and membrane stability.
Regulation of renal Na-HCO cotransporter (NBC1) activity by cholinergic agonists, ANG II, and acute acidosis (CO(2)) requires both Src family kinase (SFK) and classic MAPK pathway activation. The nonreceptor tyrosine kinase proline-rich tyrosine kinase 2 (Pyk2) couples discrete G protein-coupled receptor and growth factor receptor signaling to SFK activation. We examined the role of Pyk2-SFK interaction in coupling these stimuli to increased NBC1 activity in opossum kidney cells.
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