Microbial single-cell growth response at defined carbon limiting conditions.

Growth is one of the most fundamental characteristics of life, but detailed knowledge regarding growth at nutrient limiting conditions remains scarce. In recent years progress in microfluidic single-cell analysis and cultivation techniques has given insights into many fundamental growth characteristics such as growth homeostasis, aging and cell division of microbial cells. Using microfluidic single-cell cultivation technologies we examined how single-cell growth at defined carbon conditions, ranging from strongly limiting conditions (0.

Analyzing Microbial Population Heterogeneity-Expanding the Toolbox of Microfluidic Single-Cell Cultivations.

Recent research on population heterogeneity revealed fascinating insights into microbial behavior. In particular emerging single-cell technologies, image-based microfluidics lab-on-chip systems generate insights with spatio-temporal resolution, which are inaccessible with conventional tools. This review reports recent developments and applications of microfluidic single-cell cultivation technology, highlighting fields of broad interest such as growth, gene expression and antibiotic resistance and susceptibility.