Kinetic and thermodynamic investigation of Rhodanese synthesized by enhanced JCM 1665 strain: a comparative between the free and immobilized enzyme entrapped in alginate beads.

JCM 1665 was subjected to extracellular rhodanese production using a submerged fermentation technique. The organism was further engineered for higher cyanide tolerance and rhodanese yield using ethylmethanesulfonate as a mutagen. Mutagenesis resulted in an improved mutant with high cyanide tolerance (100 mM) and rhodanese yield (26.