Molecular Characterization, Expression Analysis, and CRISPR/Cas9 Mediated Gene Disruption of Myogenic Regulatory Factor 4 (MRF4) in Nile Tilapia.

Myogenic regulator factors (MRFs) are essential for skeletal muscle development in vertebrates, including fish. This study aimed to characterize the role of () in muscle development in Nile tilapia by cloning from muscle tissues. To explore the function of , CRISPR/Cas9 gene editing was employed.

The Isolation and Characterization of Perlucin in Pacific Abalone, : A Shell Morphogenic Protein with Potential Responses to Thermal Stress and Starvation.

Perlucin is a shell matrix protein that plays a significant role in regulating shell biomineralization. This study aimed to isolate and characterize the perlucin gene and analyze its expression to explore its role in shell formation, regeneration, and responses to thermal stress and starvation in Pacific abalone. The isolated full-length cDNA sequence of is 1002 bp long, encoding a 163-amino-acid polypeptide with a signal peptide.

The Neuropeptide HGAP Regulates Growth, Reproduction, Metamorphosis, Tissue Damage Repair, and Response against Starvation in Pacific Abalone.

Introduction: Neuropeptides regulate vital physiological processes in multicellular organisms, including growth, reproduction, metamorphosis, and feeding. Recent transcriptome analyses have revealed neuropeptide genes with potential roles in vertebrate and invertebrate growth and reproduction. Among these genes, haliotid growth-associated peptide (HGAP) was identified as a novel gene in abalone.

Optimal Target Assignment with Seamless Handovers for Networked Radars.

This paper proposes a binary linear programming formulation for multiple target assignment of a radar network and demonstrates its applicability to obtain optimal solutions using an off-the-shelf mixed-integer linear programming solver. The goal of radar resource scheduling in this paper is to assign the maximum number of targets by handing over targets between networked radar systems to overcome physical limitations such as the detection range and simultaneous tracking capability of each radar. To achieve this, time windows are generated considering the relation between each radar and target considering incoming target information.

Optimal Media Conditions for the Detection of Extracellular Cellulase Activity in Ganoderma neo-japonicum.

To determine the optimal media conditions for the detection of the extracellular cellulase activity in Ganoderma neo-japonicum, we varied three media conditions: dye reagent, pH, and temperature. We evaluated the use of four dyes, Congo red, phenol red, remazol brilliant blue, and trypan blue. To observe the effect of pH on the chromogenic reaction, we tested media ranging from 4.

Detection of Extracellular Enzyme Activities in Ganoderma neo-japonicum.

The ability of Ganoderma to produce extracellular enzymes, including β-glucosidase, cellulase, avicelase, pectinase, xylanase, protease, amylase, and ligninase was tested in chromogenic media. β-glucosidase showed the highest activity, among the eight tested enzymes. In particular, Ganoderma neo-japonicum showed significantly stronger activity for β-glucosidase than that of the other enzymes.

Optimal Medium Conditions for the Detection of Cellulolytic Activity in Ganoderma lucidum.

To determine the optimal medium conditions for the detection of the cellulolytic activity in Ganoderma lucidum, we varied three media conditions: dye reagent, pH, and temperature. First, we evaluated the use of four dyes, Congo Red, Phenol Red, Remazol Brilliant Blue, and Trypan Blue. To observe the effect of pH on the chromogenic reaction, we also made and tested various media spanning acidic and alkaline pHs, ranging from 4.

Culture Conditions for the Mycelial Growth of Ganoderma applanatum.

Ganoderma applanatum is one of the most popular medicinal mushrooms due to the various biologically active components it produces. This study was conducted to obtain basic information regarding the mycelial culture conditions of Ganoderma applanatum. Based on the colony diameter and mycelial density, PDA, YMA and MCM media were suitable for the mycelial growth of the mushroom.

Fruitbody Development of Pleurotus ostreatus via Bottle Cultivation Using Recycled Substrate.

This study was carried out to determine the possibility of bottle cultivation utilizing recycled oyster mushroom culture waste as a cultivating substrate for P. ostreatus. Total nitrogen percentage was 0.