Noninvasive and repetitive measurement of cellular metabolite from human osteosarcoma cells (MG-63) using 3.0 tesla proton ( H) MR spectroscopy.

Purpose: This study suggests a noninvasive and repetitive measurement method using H magnetic resonance spectroscopy to monitor changes in cellular metabolites within a single sample.

Methods: Longitudinal acquisition of cellular metabolites from three-dimensional cultured human osteosarcoma (MG-63) cells was conducted using 3.0 Tesla H MRS for 2 weeks at three time points: days 1, 7, and 14.