Prehospital Mass Casualty Incident Response to a Fire in a Nursing Home in Milan, Italy: Actions Taken and Shortcomings.

On July 7, 2023, at 1:21 am, a fire was declared in a retirement home in Milan, Italy. The number of casualties (n = 87) according to the Simple Triage and Rapid Treatment (START) triage system was categorized as 65 green, 14 yellow, 2 red, and 6 black; 75% were women, and the mean age was 85.1 years (± 9).

Expression of Transposable Elements in the Brain of the Model for Fragile X Syndrome.

Fragile X syndrome is a neuro-developmental disease affecting intellectual abilities and social interactions. represents a consolidated model to study neuronal pathways underlying this syndrome, especially because the model recapitulates complex behavioural phenotypes. Fragile X protein, or FMRP, is required for a normal neuronal structure and for correct synaptic differentiation in both the peripheral and central nervous systems, as well as for synaptic connectivity during development of the neuronal circuits.

Phenotyping of Fecal Microbiota of Winnie, a Rodent Model of Spontaneous Chronic Colitis, Reveals Specific Metabolic, Genotoxic, and Pro-inflammatory Properties.

Winnie, a mouse carrying a missense mutation in the MUC2 mucin gene, is a valuable model for inflammatory bowel disease (IBD) with signs and symptoms that have multiple similarities with those observed in patients with ulcerative colitis. MUC2 mucin is present in Winnie, but is not firmly compacted in a tight inner layer. Indeed, these mice develop chronic intestinal inflammation due to the primary epithelial defect with signs of mucosal damage, including thickening of muscle and mucosal layers, goblet cell loss, increased intestinal permeability, enhanced susceptibility to luminal inflammation-inducing toxins, and alteration of innervation in the distal colon.

Targeted whole exome sequencing and Drosophila modelling to unveil the molecular basis of primary ovarian insufficiency.

Study Question: Can a targeted whole exome sequencing (WES) on a cohort of women showing a primary ovarian insufficiency (POI) phenotype at a young age, combined with a study of copy number variations, identify variants in candidate genes confirming their deleterious effect on ovarian function?

Summary Answer: This integrated approach has proved effective in identifying novel candidate genes unveiling mechanisms involved in POI pathogenesis.

What Is Known Already: POI, a condition occurring in 1% of women under 40 years of age, affects women's fertility leading to a premature loss of ovarian reserve. The genetic causes of POI are highly heterogeneous and several determinants contributing to its prominent oligogenic inheritance pattern still need to be elucidated.

Predicting suitability of finger marks using machine learning techniques and examiner annotations.

Previous research has established the variability of examiners in reaching suitability determinations for friction ridge comparisons. Attempts to create predictive models to assist in this determination have been made, but have been largely confined to fully automated processes that focus on suitability for AFIS entry. This work develops, optimizes, and validates a hybrid predictive model that utilizes both examiner-observed variables and automated measures of quality and rarity to arrive at suitability classifications along four scales that have been proposed in our previous research: Value, Complexity, AFIS, and Difficulty.

Automatic assessment of fingermarks quality: Exploration of the possible application in the context of detection and comparison with human examiners.

In forensic science, particularly in the context of latent fingermarks detection, forensic scientists are often faced with the need to assess the quality of the detected fingermarks to quantitatively interpret their results and express conclusions. Today this process is mainly carried out by human examiners referring to guidelines or provided quality scales. The largest the set of fingermarks (e.

Mind-set - How bias leads to errors in friction ridge comparisons.

Mind-set is a term used in the friction ridge discipline to describe a confirmation bias in which an examiner makes early decisions about their interpretation of a mark but fails to update or reconsider those decisions in light of additional information. This most often occurs during the analysis of a mark when an examiner makes decisions (such as orientation or anatomical source of a mark) to help expedite a manual search or set parameters for an automated search, but fails to re-evaluate these decisions if the initial screening of available exemplars does not yield a comparable area, potentially leading to a miss or an erroneous exclusion. Mind-set can also occur when an examiner believes a comparison may be an identification early in the comparison process and employs poor comparison habits to convince themselves it is true, often creating or adapting comparison notes after seeing the exemplar, straining logic to justify their decision, and potentially leading to an erroneous identification.

Testing the accuracy and reliability of palmar friction ridge comparisons - A black box study.

Critics and commentators have been calling for some time for black box studies in the forensic science disciplines to establish the foundational validity of those fields-that is, to establish a discipline-wide, base-rate estimate of the error rates that may be expected in each field. While the well-known FBI/Noblis black box study has answered that call for fingerprints, no research to establish similar error rates for palmar impressions has been previously undertaken. We report the results of the first large-scale black box study to establish a discipline-wide error rate estimate for palmar comparisons.

Covid-19 and the role of smoking: the protocol of the multicentric prospective study COSMO-IT (COvid19 and SMOking in ITaly).

The emergency caused by Covid-19 pandemic raised interest in studying lifestyles and comorbidities as important determinants of poor Covid-19 prognosis. Data on tobacco smoking, alcohol consumption and obesity are still limited, while no data are available on the role of e-cigarettes and heated tobacco products (HTP). To clarify the role of tobacco smoking and other lifestyle habits on COVID-19 severity and progression, we designed a longitudinal observational study titled COvid19 and SMOking in ITaly (COSMO-IT).

SARS-CoV-2 pandemic in Italy: ethical and organizational considerations.

The current SARS-CoV-2 pandemic is still raging in Italy. The country is currently plagued by a huge burden of virusrelated cases and deaths. So far, the disease has highlighted a number of problems, some in common with other Countries and others peculiar to Italy which has suffered from a mortality rate higher than that observed in China and in most Countries in the world.

Occurrence and associative value of non-identifiable fingermarks.

Fingermarks that have insufficient characteristics for identification often have discernible characteristics that could form the basis for lesser degrees of correspondence or probability of occurrence within a population. Currently, those latent prints that experts judge to be insufficient for identification are not used as associative evidence. How often do such prints occur and what is their potential value for association? The answers are important.

Assessing Gaps in Surgical Oncology Training: Results of a Survey of General Surgery Residents.

Objective: Many general surgeons devote a significant amount of time and effort to the care of cancer patients. There is currently no standardized, national curriculum in Surgical Oncology for General Surgery residents. The objective of this study was to identify gaps in oncology education among General Surgery residents.

Characterization of a novel HLA-B*18 variant allele, HLA-B*18:122, by genome sequencing.

The HLA-B*18:122 allele showed four nucleotide differences from HLA-B*18:01:01:01.

The AIMAR recommendations for early diagnosis of chronic obstructive respiratory disease based on the WHO/GARD model*.

Respiratory diseases in Italy already now represent an emergency (they are the 3(rd) ranking cause of death in the world, and the 2(nd) if Lung cancer is included). In countries similar to our own, they result as the principal cause for a visit to the general practitioner (GP) and the second main cause after injury for recourse to Emergency Care. Their frequency is probably higher than estimated (given that respiratory diseases are currently underdiagnosed).

Administering 25-hydroxyvitamin D3 in vitamin D-deficient young type 1A diabetic patients reduces reactivity against islet autoantigens.

Background & Aims: We investigated whether improving 25-hydroxyvitamin D status in young type 1A diabetic patients reduces reactivity of peripheral blood mononuclear cells against islet autoantigens and associates with beta-cell functional changes.

Methods: Eight patients with 25-hydroxyvitamin D deficiency (<20 ng/ml), out of 15 consecutive young type 1A diabetic subjects received 25-hydroxyvitamin D3 to achieve and maintain levels above 50 ng/ml for up to one year. Peripheral blood mononuclear cell reactivity (Interferon-γ spots) against beta-cell autoantigens (glutamic acid decarboxylase 65-kD isoform, proinsulin and tyrosine phosphatase-like protein IA-2) and C-peptide during mixed meal were assessed before and after 25-hydroxyvitamin D3 replenishment.

Anaphylactic reaction after the concomitant intravenous administration of corticosteroids and gastroprotective drugs: two case reports.

Corticosteroids are therapeutic drugs widely used in cases of allergic, inflammatory and autoimmune diseases, but sometimes allergic hypersensitivity reactions have been reported as a rare adverse effect of the corticosteroids themselves. Moreover, glucocorticoids can induce gastric lesions; that’s why they are sometimes administered intravenously together with some drugs such as proton pump inhibitors (PPI) or inhibitors of histamine-2 receptors (antiH2) working as gastric protectors. Although it is difficult to establish which drug was responsible in case of hypersensitivity reactions, as hypersensitivity reactions following to the use of PPI or anti-H2 have been already described in literature.

Inhaler mishandling is very common in patients with chronic airflow obstruction and long-term home nebuliser use.

Inhalers and nebulisers are devices used for delivering aerosolised drugs in subjects with Chronic Airflow Obstruction (CAO). This multicentre, cross-sectional observational study was performed in a large population of outpatients with CAO regularly using home aerosol therapy and referring to chest clinics. The aims of the study were to compare the characteristics of the group of subjects with CAO who were using home nebulisers but also experienced with inhalers vs.

Xenotropic murine leukaemia virus-related virus is not found in peripheral blood cells from treatment-naive human immunodeficiency virus-positive patients.

The human pathogen xenotropic murine leukaemia virus-related virus (XMRV) has been tentatively associated with prostate cancer and chronic fatigue syndrome. Unfortunately, subsequent studies failed to identify the virus in various clinical settings. To determine whether XMRV circulates in humans and the relationship with its host, we searched for the virus in 124 human immunodeficiency virus-infected patients who might have been exposed to XMRV, might be prone to infection as a result of progressive immunodeficiency, and had not yet been treated with antiretroviral drugs.

Cancer transmissibility across HLA barriers between immunocompetent individuals: rare but not impossible.

The axiom of human leukocyte incompatibility (HLA) incompatibility has always led scientists to consider cancer transmission between HLA-different individuals impossible. In fact, cancer transmission between individuals represents a frightening possibility in animal populations with limited HLA diversity or for rare cancers exploiting downregulation of HLA expression. We review here evidence from nonhuman models and settings for interhuman transmission.

Determination of topiramate in human plasma by capillary electrophoresis with indirect UV detection.

A rapid capillary zone electrophoresis method with indirect UV detection for the determination of topiramate in human plasma was developed and validated. The analyses were carried out with a background electrolyte composed of 10mM sulfamethoxazole as chromophore in phosphate buffer (25 mM, pH 12.0); gabapentin was selected as the internal standard.