Publications by authors named "Dongyang Wang"

Aim: To construct the recombinant adenoviral vectors expressing human endostatin, K5 and endostatin-K5 gene respectively, and study their bioactivity in vitro.

Methods: Human endostatin, K5 and endostatin-K5 gene were amplified by PCR, which were then subcloned into shuttle vector pAd5-CMV-H1H2-MCS-6His by enzyme and ligation respectively. The positive recombinant plasmids linearized by Pac I were cotransfected into HEK 293 cells with the Pac I linearized adenoviral backbone plasmid using calcium phosphate precipitation method.

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The transduction efficiency of Ad (adenovirus) depends, to some extent, on the expression level of CAR (coxsackievirus and Ad receptor) of a target cell. The low level of CAR on the cell surface is a potential barrier to efficient gene transfer. To overcome this problem, PTD.

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Aim: To construct the vector for efficient expression of siRNA using pre-mir30 backbone.

Methods: By chemical synthesis method, pre-mir30 backbone introduced an appropriate restriction enzyme site for foreign shRNA inserting was cloned into an expressing vector containing U6 promoter. The silencing efficiency of a new siRNA expressing vector was detected by transfection and Western blot.

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Synopsis of recent research by authors named "Dongyang Wang"

  • - Dongyang Wang's recent research spans the fields of polymeric thermoelectrics and the molecular mechanisms of disease, with a focus on advancing high-performance materials for electronic applications through innovative doping methods and structural designs.
  • - His studies introduced novel techniques such as photoexcitation-assisted molecular doping and imprinted porous polymer films, which significantly enhance thermoelectric performance and provide aesthetically interesting materials for next-generation electronics.
  • - Wang also contributes to understanding genetic influences on diseases, such as the impacts of COVID-19 on cognitive functions and the development of comprehensive databases for miRNA-related SNPs across various species, showcasing a multidisciplinary approach to health and materials science.