CRISPR/Cas9-mediated mutagenesis of results in enhanced resistance to powdery mildew in grapevine ().

Grapevine (), one of the most economically important fruit crops in the world, suffers significant yield losses from powdery mildew, a major fungal disease caused by . In addition to suppressing host immunity, phytopathogens modulate host proteins termed susceptibility (S) factors to promote their proliferation in plants. In this study, CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated 9) technology was used to enable the targeted mutagenesis of (mildew resistance Locus O) family genes that are thought to serve as factors for powdery mildew fungi.

Ectopic expression of Arabidopsis broad-spectrum resistance gene RPW8.2 improves the resistance to powdery mildew in grapevine (Vitis vinifera).

Powdery mildew is the most economically important disease of cultivated grapevines worldwide. Here, we report that the Arabidopsis broad-spectrum disease resistance gene RPW8.2 could improve resistance to powdery mildew in Vitis vinifera cv.