LncRNA81246 regulates resistance against tea leaf spot by interrupting the miR164d-mediated degradation of NAC1.

Non-coding RNAs play crucial roles in plant responses to viral stresses. However, their molecular mechanisms in tea leaf spot responses remain unclear. In this study, using Camellia sinensis, we identified lncRNA81246 as a long non-coding RNA that localizes to both the nucleus and cytoplasm.

The use of electromagnetic navigation bronchoscopy-guided microwave ablation in patients with multiple bilateral pulmonary nodules: a retrospective study of 26 cases.

Background: To treat multiple bilateral ground-glass opacities (GGOs), surgical treatments and electromagnetic navigation bronchoscopy (ENB)-guided ablation therapy are recommended therapeutic measures. However, the differences between bilateral and unilateral ablation, with or without surgery, remain unknown. This study aims to evaluate the differences in efficacy among various strategies.

Constructing perfluorinated UiO-67 for enrichment of polycyclic aromatic hydrocarbons in seawater and seabed sediments.

To investigate the ocean contamination caused by polycyclic aromatic hydrocarbons (PAHs), UiO-67/perfluorooctanoic acid (UiO-67/PFOA) was synthesized through solvent-assisted ligand incorporation method. The UiO-67/PFOA was then served as an adsorbent in headspace solid-phase microextraction (HS-SPME) technology for collecting and concentrating trace PAHs. The addition of the PFOA improved the hydrophobicity and stability of the UiO-67/PFOA coating, and the C-F functional group in UiO-67/PFOA could form the pseudo hydrogen bonding with the CH on the benzene ring of PAHs, which endowed the UiO-67/PFOA with 1.

Boronic acid functionalized of covalent organic framework for high performance capture of trace phthalates.

In order to improve the enrichment performance of parent covalent organic frameworks (COFs), boronic acid functionalized of COF (COF-B(OH)) was obtained by a simple standing method for the first time. The obtained COF-B(OH) exhibited the new characteristics that were not possessed by pure COF and was employed as the solid phase microextraction (SPME) coating material for highly efficient enrichment of trace endocrine disruptors phthalates (PAEs). Compared to pure COF, the synergistic effect of the newly emerged unique pore structure and boric acid interaction sites, and the large specific surface area and the abundant benzene ring structure inherited by original COF framework endowed COF-B(OH) with enhanced enrichment performance for PAEs.

The Microbial Metabolite Wuyiencin Potential Targets Threonine dehydratase in to Achieve Control of Tea Leaf Spot.

Tea leaf spot caused by is a disease that has recently been discovered in the tea plantations of Southwest China, and which has a significant negative impact on the yield and quality of tea leaves. Wuyiencin is a nucleotide antimicrobial that is effective against a range of fungal diseases. However, its mode of action is still unclear.

Phenazine-1-carboxamide Regulates Pyruvate Dehydrogenase of Phytopathogenic Fungi to Control Tea Leaf Spot Caused by .

Due to a lack of understanding of the disease epidemiology and comprehensive control measures, tea leaf spot caused by has a significant negative impact on tea yield and quality in the tea plantations of Southwest China. Phenazine-1-carboxamide (PCN) is a phenazine compound derived from species, which exhibits antimicrobial activity against various pathogens. However, its inhibitory mechanism is not yet clear.

CYP3A4 gene expression discloses individual differences in postoperative pain susceptibility and drug treatment response in patients with lung cancer.

This study investigates the influence of CYP3A4 gene polymorphisms on postoperative pain sensitivity and analgesic response in lung cancer patients undergoing intercostal nerve block with local anesthetics. Sixty patients (ages 31-74) undergoing thoracoscopic lung cancer surgery were enrolled and divided into two groups based on CYP3A4 gene expression level: Group I (high CYP3A4) and Group II (low CYP3A4). Postoperative pain was assessed using the Visual Analogue Scale (VAS), and patient-controlled intravenous analgesia (PCIA) pump usage, ECG ST-T segment changes, complications, hospital stay, and costs were recorded.

Pyriofenone Interacts with the Major Facilitator Superfamily Transporter of Phytopathogenic Fungi to Potentially Control Tea Leaf Spot Caused by .

Tea leaf spot caused by is a newly discovered fungal disease in southwest China. Due to a lack of knowledge of its epidemiology and control strategies, the disease has a marked impact on tea yield and quality. Pyriofenone is a new fungicide belonging to the aryl phenyl ketone fungicide group, which has shown marked efficacy in controlling various fungal diseases.

Proteome and Phosphoproteome Profiling Reveal the Toxic Mechanism of Epsilon Toxin in MDCK Cells.

Epsilon toxin (ETX), a potential agent of biological and toxic warfare, causes the death of many ruminants and threatens human health. It is crucial to understand the toxic mechanism of such a highly lethal and rapid course toxin. In this study, we detected the effects of ETX on the proteome and phosphoproteome of MDCK cells after 10 min and 30 min.

Gibberellin 2-oxidase 1(CsGA2ox1) involved gibberellin biosynthesis regulates sprouting time in camellia sinensis.

Background: Tea is an important cash crop and buds are its main product. To elucidate the molecular mechanism of the sprouting time of tea plants, 'Yuchunzao', which was an early sprouting tea cultivar, was studied. 'Echa 1', sprout one week later than 'Yuchunzao' in spring, was used as the control.

The effect of land use and land cover on soil carbon storage in the Yellow River Delta, China: Implications for wetland restoration and adaptive management.

Gaining a comprehensive understanding of the effect of land use/land cover (LULC) and soil depth on soil carbon storage, through the manipulation of external carbon input and turnover processes, is crucial for accurate predictions of regional soil carbon storage. Numerous research investigations have been conducted to examine the impact of LULC on the storage and cycling of carbon in the surface soils of coastal wetlands. Nevertheless, there remains a dearth of understanding concerning the implications of this phenomenon on subterranean soils, a crucial factor in discerning the capacity for carbon sequestration in coastal wetlands and implementing measures for their preservation.

[Cell membrane-coated nanoparticles in disease therapy].

Nanotechnology has attracted increasing attention in the field of medical applications due to its significant potential for development. However, one major challenge that has emerged with nanoparticles is their tendency to activate the host immune clearance system, which hampers the achievement of desired therapeutic outcomes and may lead to harmful side effects. In recent years, membrane-coated nanoparticles have emerged as a promising solution, demonstrating their effectiveness in evading immune system clearance.

A combinatorial MRI sequence-based radiomics model for preoperative prediction of microsatellite instability status in rectal cancer.

This study aimed to develop an optimal radiomics model for preoperatively predicting microsatellite instability (MSI) in patients with rectal cancer (RC) based on multiparametric magnetic resonance imaging. The retrospective study included 308 RC patients who did not receive preoperative antitumor therapy, among whom 51 had MSI. Radiomics features were extracted and dimensionally reduced from T2-weighted imaging (T2WI), T1-weighted imaging (T1WI), diffusion-weighted imaging (DWI), and T1-weighted contrast enhanced (T1CE) images for each patient, and the features of each sequence were combined.

Phosphorylation of FOXK2 at Thr13 and Ser30 by PDK2 sustains glycolysis through a positive feedback manner in ovarian cancer.

Ovarian cancer is one of the most common gynecological malignant tumors with insidious onset, strong invasiveness, and poor prognosis. Metabolic alteration, particularly aerobic glycolysis, which is tightly regulated by transcription factors, is associated with the malignant behavior of OC. We screened FOXK2 in this study as a key transcription factor that regulates glycolysis in OC.

MOF-derived porous carbon microspheres Ni@C-acid as solid-phase microextraction coating for extraction of polycyclic aromatic hydrocarbons from tea infusions.

The improvement of the stability and adsorption properties of materials on targets in sample pre-treatment has long been an objective. Extensive efforts have been made to achieve this goal. In this work, metal-organic framework Ni-MOF precursors were first synthesized by solvothermal method using polyvinylpyrrolidone (PVP) as an ideal templating agent, stabiliser and nanoparticle dispersant.

Plant polysaccharides extracted by high pressure: A review on yields, physicochemical, structure properties, and bioactivities.

Polysaccharides are biologically essential macromolecules, widely exist in plants, which are used in food, medicine, bioactives' encapsulation, targeted delivery and other fields. Suitable extraction technology can not only improve the yield, but also regulate the physicochemical, improve the functional property, and is the basis for the research and application of polysaccharide. High pressure (HP) extraction (HPE) induces the breakage of raw material cells and tissues through rapid changes in pressure, increases extraction yield, reduces extraction time, and modifies structure of polysaccharides.

Desmoglein-2 Affects Vascular Function in Moyamoya Disease by Interacting with MMP-9 and Influencing PI3K Signaling.

The pathogenesis and development of Moyamoya disease are still unclear. This study aimed to investigate the effect of desmoglein-2 (DSG2) on Moyamoya disease and determine the inhibitory effect of DSG2 in vascular remodeling in Moyamoya disease.RNA sequencing, immunohistochemistry (IHC), and western blotting were used to detect the expression of DSG2 in the superficial temporal artery (STA) tissues of Moyamoya disease.

A GAPDH serotonylation system couples CD8 T cell glycolytic metabolism to antitumor immunity.

Apart from the canonical serotonin (5-hydroxytryptamine [5-HT])-receptor signaling transduction pattern, 5-HT-involved post-translational serotonylation has recently been noted. Here, we report a glyceraldehyde-3-phosphate dehydrogenase (GAPDH) serotonylation system that promotes the glycolytic metabolism and antitumor immune activity of CD8 T cells. Tissue transglutaminase 2 (TGM2) transfers 5-HT to GAPDH glutamine 262 and catalyzes the serotonylation reaction.

Stabilization of Pin1 by USP34 promotes Ubc9 isomerization and protein sumoylation in glioma stem cells.

The peptidyl-prolyl cis-trans isomerase Pin1 is a pivotal therapeutic target in cancers, but the regulation of Pin1 protein stability is largely unknown. High Pin1 expression is associated with SUMO1-modified protein hypersumoylation in glioma stem cells (GSCs), but the underlying mechanisms remain elusive. Here we demonstrate that Pin1 is deubiquitinated and stabilized by USP34, which promotes isomerization of the sole SUMO E2 enzyme Ubc9, leading to SUMO1-modified hypersumoylation to support GSC maintenance.

Prediction of Mild Cognitive Impairment Progression to Alzheimer's Disease Based on Diffusion Tensor Imaging-Derived Diffusion Parameters: Construction and Validation of a Nomogram.

Introduction: The aim of the study was to construct and validate a nomogram that combines diffusion tensor imaging (DTI) parameters and clinically relevant features for predicting the progression of mild cognitive impairment (MCI) to Alzheimer's disease (AD).

Method: A retrospective analysis was conducted on the MRI and clinical data of 121 MCI patients, of whom 32 progressed to AD during a 4-year follow-up period. The MCI patients were divided into training and validation sets at a ratio of 7:3.