Effects of tumor necrosis factor-α on glucose uptake in human granulosa cells under high androgen conditions.

Objectives: Hyperandrogenism is a key pathological characteristic of polycystic ovary syndrome (PCOS). Tumor necrosis factor α (TNF-α) is both an adipokine and a chronic inflammatory factor, which has been proven to be involved in the pathologic process of PCOS. This study aimed to determine how TNF-α affects glucose uptake in human granulosa cells in the presence of high testosterone concentration.

[Meta-analysis and GRADE evaluation of Guanxinning Tablets in treatment of angina pectoris of coronary heart disease].

This study aims to evaluate the efficacy and safety of Guanxinning Tablets+conventional western medicine in the treatment of angina pectoris of coronary heart disease, and provide evidence-based references for clinical medication. Retrieved from CNKI, Wanfang, VIP, SinoMed, PubMed, EMbase, Cochrane Library, randomized controlled trial(RCT) about Guanxinning Tablets for the treatment of angina pectoris of coronary heart disease from the inception to April 2022 were collected. After literature screening and data extraction, the bias risk assessment tool recommended by the Cochrane evaluation manual handbook 5.

Word's Predictability Can Modulate Semantic Preview Effect in High-Constraint Sentences.

The processing of words in sentence reading is influenced by both information from sentential context (the effect of predictability) and information from previewing upcoming words (the preview effect), but how both effects interact during online reading is not clear. In this study, we tested the interaction of predictability effect and the preview effect in predicting reading processing. In the experiment, sentence constraint was controlled using all high-constraint sentences as materials.

Long Noncoding RNA X-Inactive Specific Transcript Facilitates Cellular Functions in Melanoma via miR-139-5p/ROCK1 Pathway.

Purpose: Although X-inactive specific transcript (XIST) is known to play a critical role in the pathogenesis of melanoma, the mechanisms through which this remains unclear.

Methods: RNAseq, immunohistochemistry, and qRT-PCR were used to identify the levels of XIST, miR-139-5p, and Rho-Associated Coiled-Coil Containing Protein Kinase-1 (ROCK1) in melanoma tissues and cells. A subcellular fractionation assay was used to determine the location of XIST.

Retracted: MicroRNA-373 promotes the development of endometrial cancer by targeting LATS2 and activating the Wnt/β-Catenin pathway.

In the female reproductive tract, endometrial cancer is the most common malignant tumor. Recently, the specific functions of many miRNAs have been identified in endometrial cancer. However, the contradictory effects of microRNA-373 (miR-373) in different human cancers draw our attention.

[Preparation and characterization of the monoclonal antibody against human soluble mesothelin-related proteins].

Aim: To prepare the monoclonal antibody (mAb) against human soluble mesothelin-related proteins (SMR) and identify its properties.

Methods: The B-cell epitopes of mesothelin (MSLN) were predicted by multi-parameter prediction method. Then BALB/c mice were immunized with compound MSLN polypeptide prepare the mAb by hybridoma technique.