Effects of Different Cultivation Modes on Morphological Traits and Correlations between Traits and Body Mass of Crayfish ().

In this study, juvenile crayfish hatched from the same population were cultured in different growing environments: pond (), paddy field (), and aquaculture barrel (), and fed for 60 days. Crayfishes were selected randomly, females and males, 50 tails each from six groups (D1-♀, D1-♂, D2-♀, D2-♂, D3-♀, D3-♂) to measure the following morphological traits: full length (), body length (), chelicerae length (), chelicerae weight (), cephalothorax length (), cephalothorax width (), cephalothorax height (), eye spacing (), caudal peduncle length (), and caudal peduncle weight (). We found that the coefficient of variation (CV) of was the largest in each culture mode, and males (28.

Systematic screening of hepatoprotective components from traditional Chinese medicine: Zuojin Pill as an example.

Ethnopharmacological Relevance: Zuojin Pill (ZJP), composed of Coptis chinensis Franch. and Euodia ruticarpa (A. Juss.

Study on quality control of Zuojin pill by HPLC fingerprint with quantitative analysis of multi-components by single marker method and antioxidant activity analysis.

Current quality control methods for Zuojin Pill (ZJP) lack comprehensiveness and practicability. This study aimed to develop a comprehensive strategy for the quality evaluation of ZJP and the prediction of potential bioactive components in ZJP. First, an HPLC method with excellent separation of main components was developed and was used to establish the chromatographic fingerprint of ZJP.

National-scale urinary phthalate metabolites in the general urban residents involving 26 provincial capital cities in China and the influencing factors as well as non-carcinogenic risks.

Phthalates (PAEs) are widely used in daily products but can cause a variety of adverse effects in humans. Few studies have been carried out on human internal exposure levels of PAEs on a large-scale, especially in developing countries. In the present study, 1161 urine samples collected from residents of 26 provincial capitals in China were analyzed for nine phthalate metabolites (mPAEs).

Conducting large, repeated, multi-game economic experiments using mobile platforms.

We demonstrate the possibility of conducting synchronous, repeated, multi-game economic decision-making experiments with hundreds of subjects in-person or remotely with live streaming using entirely mobile platforms. Our experiment provides important proof-of-concept that such experiments are not only possible, but yield recognizable results as well as new insights, blurring the line between laboratory and field experiments. Specifically, our findings from 8 different experimental economics games and tasks replicate existing results from traditional laboratory experiments despite the fact that subjects play those games/task in a specific order and regardless of whether the experiment was conducted in person or remotely.

Fast Screening of Biomembrane-Permeable Compounds in Herbal Medicines Using Bubble-Generating Magnetic Liposomes Coupled with LC-MS.

A novel strategy based on the use of bionic membrane camouflaged magnetic particles and LC-MS was developed to quickly screen the biomembrane-permeable compounds in herbal medicines. The bionic membrane was constructed by bubble-generating magnetic liposomes loaded with NHHCO (BMLs). The lipid bilayer structure of the liposomes enabled BMLs to capture biomembrane-permeable compounds from a herbal extract.

Improving rare disease classification using imperfect knowledge graph.

Background: Accurately recognizing rare diseases based on symptom description is an important task in patient triage, early risk stratification, and target therapies. However, due to the very nature of rare diseases, the lack of historical data poses a great challenge to machine learning-based approaches. On the other hand, medical knowledge in automatically constructed knowledge graphs (KGs) has the potential to compensate the lack of labeled training examples.

The complete mitochondrial genome of the hybrid [ (♀) × (♂)].

The complete mitochondrial genome of the hybrid of (♀) (♂) was characterized first in this study. The total length of the mitochondrial genome of was identical to the female parent as 16,581 bp, and the overall base composition was 31.87% A, 24.

The complete mitochondrial genome of the hybrid (♀)× (♂)].

The complete mitochondrial genome of the hybrid of (♀)×  (♂) was characterized first in this study. The total length of the genome was identical to the female parent as 16,581 bp, and the overall base composition was 31.80% A, 24.

Base composition of the Danio chrysotaenitus complete mitochondrial genome.

In this study, 16 sets of primers were used to amplify contiguous, overlapping segments of the complete mitochondrial DNA (mtDNA) of Danio chrysotaenitus in order to characterize and compare their mitochondrial genomes. The total length of the mitochondrial genome is 16,608 bp and deposited in the GenBank with accession numbers KP407138. The organization of the mitochondrial genomes was similar to those reported from other Mountain carp fishes mitochondrial genomes containing 37 genes (13 protein-coding genes, 2 ribosomal RNA, and 22 transfer RNAs) and a major non-coding control region (D-loop region).

Thyroxine and reserpine-induced changes in metabolic profiles of rat urine and the therapeutic effect of Liu Wei Di Huang Wan detected by UPLC-HDMS.

The promise of metabonomics, a new "omics" technique, to validate Chinese medicines and the compatibility of Chinese formulas has been appreciated. The present study was undertaken to explore the excretion pattern of low molecular mass metabolites in the male Wistar-derived rat model of kidney yin deficiency induced with thyroxine and reserpine as well as the therapeutic effect of Liu Wei Di Huang Wan (LW) and its separated prescriptions, a classic traditional Chinese medicine formula for treating kidney yin deficiency in China. The study utilized ultra-performance liquid chromatography/electrospray ionization synapt high definition mass spectrometry (UPLC/ESI-SYNAPT-HDMS) in both negative and positive electrospray ionization (ESI).

In vitro study of the effects of lens extract on rat retinal neuron survival and neurite outgrowth.

Background: Optic nerve regeneration has previously been achieved by injuring the lens, which results in the release of lentogenic factors. However, these lentogenic factors are still unknown.

Objectives: To investigate what were the lentogenic factors by examining the effects of lens extract and macrophage-conditioned medium (MCM) on the survival and the neurite outgrowth of rat retinal neurons in vitro.

Distribution and expression of RhoA in rat retina after optic nerve injury.

Background: RhoA is a small guanosine triphosphatase which participates in signaling pathways of axonal repellents or inhibitors. However, the distribution and expression of RhoA in the rat retina after optic nerve injury has not been elucidated yet.

Objectives: To study the distribution and expression of RhoA in the rat retina after optic nerve injury.

[Influence of fluorescent protein expression on the proliferation of NIH3T3 cells in vitro].

Objective: To investigate the influence of fluorescent protein expression on the proliferation of murine NIH3T3 cells, so as to provide a theoretical basis for cell tracing technology.

Methods: NIH3T3 cells were cultured in vitro, and were randomly divided into control, pLEGFP-N1 (with transfection of pLEGFP-N1 retroviral vector), pEGFP-N1 (with transfection of pEGFP-N1 vector) and pDsRed2-C1 (with transfection of pDsRed2-C1 vector) groups. Then the cells were screened by G418 for 3 weeks.