Genome-scale transcriptomic insights into the gene co-expression network of seed abortion in triploid Siraitia grosvenorii.

Background: Siraitia grosvenorii (Swingle) C. Jeffrey, also known as Luohanguo or monk fruit, is a famous traditional Chinese medicine ingredient with important medicinal value and broad development prospects. Diploid S.

[Variety and standard arrangement of Yao medicine in Euphorbiaceae].

The names, basic sources, medicinal parts, efficacy and standards of the medicinal materials in Euphorbiaceae were systematically collated and analyzed by the textual research for Yao medicine monographs in this paper. The results showed that there were great differences in the names, basic sources, medicinal parts and efficacy of some medicinal materials recorded in different literatures. There were 19 genera and 60 species(including varieties) of Euphorbiaceae of Yao medicine, involving 50 kinds of medicinal materials.

The effect of 8-OH-DPAT and dapoxetine on gene expression in the brain of male rats during ejaculation.

The 5-HT receptor agonist 8-hydroxy-2-[di--propylamino] tetralin (8-OH-DPAT) promotes ejaculation of male rats, whereas dapoxetine delays this process. However, the gene expression profile of the brain at ejaculation following administrationof these two compounds has not been fully elucidated. In the present study, a transcriptomic BodyMap was generated by conducting mRNA-Seq on brain samples of male Sprague-Dawley rats.

Cloning and characterization of squalene synthase and cycloartenol synthase from .

Mogrosides and steroid saponins are tetracyclic triterpenoids found in . Squalene synthase (SQS) and cycloartenol synthase (CAS) are key enzymes in triterpenoid and steroid biosynthesis. In this study, full-length cDNAs of and were cloned by a rapid amplification of cDNA-ends with polymerase chain reaction (RACE-PCR) approach.

Profiles of brain central nervous system gene expression associated with ejaculation behavior in male rats.

The male rat has been used extensively as a model for evaluating the neurophysiology of sexual behavior. However, gene expression in the brain throughout the process of sexual intercourse has yet to be elucidated. In the present study, we created a transcriptomic BodyMap by performing mRNA-Seq on brain samples from pre-copulatory control (CK), fourth intromission (CR4), ejaculation (EJ) and post-ejaculatory interval 1-min (PEI1) Sprague Dawley rats (n=40, all male, each 10).

Cloning and expression of from .

CYP450 plays an essential role in the development and growth of the fruits of . However, little is known about the gene in . Here, based on transcriptome data, a full-length cDNA sequence of was cloned by reverse transcriptase-polymerase chain reaction (RT-PCR) and rapid-amplification of cDNA ends (RACE) strategies.

[Study on exogenous hormones inducing parthenocarpy fruit growth and development and quality of Siraitia grosvenorii].

To explore the growth and development and analyze the quality of the parthenocarpy fruit induced by exogenous hormones of Siraitia grosvenorii. the horizontal and vertical diameter, volume of the fruit were respectively measured by morphological and the content of endogenous hormones were determined by ELISA. The size and seed and content of mogrosides of mature fruit were determined.

Identification of novel and conserved microRNAs in Panax notoginseng roots by high-throughput sequencing.

Background: MicroRNAs (miRNAs) are small, non-coding RNAs that are important regulators of gene expression, and play major roles in plant development and their response to the environment. Root extracts from Panax notoginseng contain triterpene saponins as their principal bioactive constituent, and demonstrate medicinal properties. To investigate the novel and conserved miRNAs in P.

[Selection of reference genes of Siraitia grosvenorii by real-time PCR].

Siraitia grosvenorii is a traditional Chinese medicine also as edible food. This study selected six candidate reference genes by real-time quantitative PCR, the expression stability of the candidate reference genes in the different samples was analyzed by using the software and methods of geNorm, NormFinder, BestKeeper, Delta CT method and RefFinder, reference genes for S. grosvenorii were selected for the first time.