Analysis of differential metabolites and metabolic pathways in adipose tissue of tree shrews (Tupaia belangeri) under gradient cooling acclimation.

In order to investigate the influence of gradient cooling acclimation on body mass regulation in tree shrews (Tupaia belangeri), white adipose tissue (WAT) and brown adipose tissue (BAT) in T. belangeri between the control group and gradient cooling acclimation group on day 56 were collected, body mass, food intake, thermogenic capacity, differential metabolites, and related metabolic pathways in WAT and BAT were measured, the changes of differential metabolites were analyzed by non-targeted metabolomics method based on liquid chromatography-mass spectrometry. The results shown that gradient cooling acclimation significantly increased body mass, food intake, resting metabolic rate (RMR), non-shivering thermogenesis (NST), and masses of WAT and BAT.

Genomic insights into the evolution of the critically endangered soft-shelled turtle Rafetus swinhoei.

The 2019 death of the female Swinhoes soft-shelled turtle (Rafetus swinhoei) individual in China put this species in a dire conservation state. To avoid the loss of genetic information from the female individual and facilitate the protection and preservation of R. swinhoei, we sequenced and assembled its draft chromosome-level genome by combining BGISEQ short reads, Nanopore long reads, and Hi-C sequencing reads.

Metabonomics of white adipose tissue and brown adipose tissue in Tupaia belangeri during cold acclimation.

In the present study, liquid chromatography-mass spectrometer (LC-MS) was used to perform untargeted metabolomics analysis of white adipose tissue (WAT) and brown adipose tissue (BAT) in Tupaia belangeri during cold acclimation. Differences in biochemical composition between WAT and BAT were compared. Clarifying how the two adipose tissues respond to the lower temperature in terms of metabolomics, which elucidate the metabolic process and energy homeostasis regulation mechanism in T.

Metabolomics on serum levels and liver of male Tupaia belangeri from 12 locations in China by GC-MS.

Objectives: To investigate adaptive strategies of Tupaia belangeri to environmental factors in different populations, 12 locations were selected, including higher and lower altitude areas.

Results: Total of 96 and 90 metabolites were annotated in serum and liver respectively, which were mainly concentrated in primary metabolites. Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) showed that the locations were divided into two groups in serum metabolites, but each group had a few samples overlap.

[Evaluation of different ultrasonic modalities for prenatal screening congenital heart diseases].

Objective: To evaluate the value of four chamber view, outflow tract view and color Doppler echocardiography for detecting the fetal congenital heart defects among high-risk populations. And to discuss the significance of screening congenital heart defects by three methods and select the suitable method for wider applications.

Methods: The echocardiographic records of all pregnant women performed at our hospital from January 2006 to December 2007 were reviewed retrospectively by screening of clinical epidemiology.

[Early diagnosis of endometrial disorder in women with postmenopausal bleeding by three-dimensional transvaginal sonography and hysterosonography].

Objective: To evaluate the value of three-dimensional transvaginal sonography and hysterosonography in the diagnosis of endometrial lesion in women with postmenopausal bleeding.

Methods: A total of 74 patients with postmenopausal bleeding were prospectively studied by two- and three-dimensional transvaginal sonography. Endometrial thickness and volume were measured by 2D and 3D ultrasound respectively.

[Prenatal ultrasonography for fetus with hydronephrosis].

Objective: To investigate the relationship between classification and prognosis of hydronephrosis in fetus.

Methods: 226 pregnant women in their pregnant weeks 20 - 40 who were suggested to be with fetuses suffering from hydronephrosis underwent ultrasonography to observe the configuration of the kidneys, form and size of renal pelvis, extent of calyces, and thickness of renal cortex of their fetuses. The ultrasonography was conducted regularly and the outcome after birth was followed up.