NiFe Nanoalloy Electrocatalyst as a Coreaction Accelerator for Enhancing Biomarker Electrochemiluminescence Imaging Detection.

The development of coreaction accelerators with excellent performance is crucial for improving electrochemiluminescence (ECL) performance. In this study, a NiFe nanoalloy electrocatalyst (NiFe-NAEC) was used as a coreaction accelerator to promote persulfate activation, resulting in a 44-fold increase in the ECL intensity of flower-like Zn-3,4,9,10-perylenetetracarboxylate (FL-Zn-PTC). Moreover, the synergistic combination of NiFe-NAEC and silver nanoparticles led to a 134-fold increase in the ECL intensity of FL-Zn-PTC, with a 6275% improvement in the ECL efficiency compared with a reference system containing Ru-bpy/KSO.

Aggregation-induced electrochemiluminescence enhancement of Ag-MOG for amyloid β 42 sensing.

This study aimed to introduce an immunosensor for measuring amyloid β 42 (Aβ) levels by aggregation-induced enhanced electrochemiluminescence (ECL). Metal-organic gels (MOGs) are novel soft materials with advantages such as high gel stability, good light-emitting properties, and easy preparation. This study used silver nanoparticle metal-organic gel (Ag-MOG) as a substrate to connect Aβ-Ab2 and the cathodoluminescent probe.

Novel immunosensor based on electrochemiluminescence inner filter effect and static quenching between fibrillary Ag-MOGs and SiO@PANI@AuNPs for enabling the sensitive detection of neuron-specific enolase.

Metal-organic gels (MOGs) are unique supramolecular gels that are convenient to synthesize. In this work, a cathodic electrochemiluminescence (ECL) system based on Ag-MOGs as a luminophore and KSO as a co-reactor was developed. The ECL spectrum of the Ag-MOGs overlapped significantly with the strong UV-Vis spectrum of the SiO@PANI@AuNPs, which effectively quenched the ECL luminescence of the Ag-MOGs.

Electrochemiluminescence immunosensor based on Cu(PO) hybrid nanoflowers as a novel luminophore for the sensitive detection of prostate-specific antigen.

Copper phosphate hybrid nanoflowers (Cu(PO)HNFs) were demonstrated to produce cathodic ECL emission in the presence of potassium persulfate (KSO) and then used as a carrier due to their large specific surface area. AgNPs modified on Cu(PO)HNFs provided more binding sites for immobilizing secondary antibodies and accelerating the electron transfer rate to enhance the ECL signal. In addition, FONDs-Au was used to capture primary antibodies due to its good biocompatibility and large specific surface area.

Electrochemiluminescence resonance energy transfer between a Ru-ZnMOF self-enhanced luminophore and a double quencher ZnONF@PDA to detect NSE.

The construction of advanced systems capable of accurately detecting neuron-specific enolase (NSE) is essential for rapidly diagnosing small-cell lung cancer. In this study, an electrochemiluminescence (ECL) resonance energy transfer immunosensor was proposed for the ultra-sensitive detection of NSE. The co-reactants CO and Ru(bpy) were integrated to form a self-enhanced ECL luminophore (Ru-ZnMOF) as the ECL donor.

Dual-potential electrochemiluminescence cytosensor based on a metal-organic framework and ABEI-PEI-Au@AgNPs for the simultaneous determination of phosphatidylserine and epidermal growth factor receptors on an apoptotic cell surface.

A new electrochemiluminescence (ECL) cytosensor is proposed for the simultaneous determination of phosphatidylserine (PS) and epidermal growth factor receptor (EGFR) based on the ECL signals of metal-organic framework-5 (MOF-5) loaded CdS quantum dots and N-(aminobutyl)-N-(ethylisoluminol)-polyethylenimine capped Au and Ag nanoparticles. Apoptosis promotes the exposure of PS and reduces the expression of EGFR in cell membranes. Two spatially resolved areas on dual-disk glassy carbon electrodes were designed to eliminate the interference from different ECL probes.

Au@NiFeMOFs as the signal quencher of Au@g-CNNSs composite for sensitive "on-off" electrochemiluminescence immunosensing of beta-2-microglobulin.

In this study, an electrochemiluminescence resonance energy transfer (ECL-RET) immunosensor was constructed to detect beta-2-microglobulin (B2M). As a donor-acceptor pair, a carbon nitride nanosheet modified with gold nanoparticles (Au@g-CNNSs) and a nickel- and iron-based organic framework modified with gold nanoparticles (Au@NiFeMOFs) were prepared. The sandwich immunosensor was successfully constructed so that ECL-RET occurred between Au@NiFeMOFs and Au@g-CNNSs.

An electrochemiluminescence immunosensor with double co-reaction accelerators based on AgPO@EuPO-AgNP for detecting squamous cell carcinoma antigen.

This study aimed to design a sandwich electrochemiluminescence (ECL) immunosensor with double co-reaction accelerators for sensitively detecting squamous cell carcinoma antigen (SCCA). First, silver orthophosphate (AgPO) nanoparticles were modified on the surface of EuPO nanowires to improve their poor dispersibility/solubility. At the same time, EuPO was used as a co-reaction accelerator to catalyze SO to produce more intermediates (SO), significantly enhancing the ECL signal of AgPO.

Aggregation-Induced Electrochemiluminescence Based on a Zinc-Based Metal-Organic Framework and a Double Quencher Au@UiO-66-NH for the Sensitive Detection of Amyloid β 42 Resonance Energy Transfer.

A novel sandwich electrochemiluminescence (ECL) biosensor based on aggregation-induced electrochemiluminescence resonance energy transfer (AIECL-RET) was designed for the sensitive detection of amyloid β42 (Aβ42). The synthesized silver nanoparticle-functionalized zinc metal-organic framework (Ag@ZnPTC) and gold nanoparticle-functionalized zirconium organic framework (Au@UiO-66-NH) were used as the ECL donor and acceptor, respectively. AgNPs were generated on the surface of ZnPTC, which further improved the ECL intensity and the loading of antibody 1 (Ab1).

A dual-emitting immunosensor based on manganese dioxide nanoflowers and zinc sulfide quantum dots with enhanced electrochemiluminescence performance for the ultrasensitive detection of procalcitonin.

A dual-emitting electrochemiluminescence (ECL) immunosensor based on manganese dioxide nanoflowers (MnONFs) and zinc sulfide quantum dots (ZnSQDs) was constructed for the first time to sensitively detect procalcitonin (PCT) in human serum. rGO@Ag functioned not only to adsorb primary antibodies (Ab1) but also to improve the electrical conductivity of the immunosensor. The MnONFs and ZnSQDs in the nanocomposite, synergistically with silver nanoparticles, simultaneously functioned as cathodic ECL emitters to enhance the detection sensitivity of PCT by shortening the electron-transfer path, thereby reducing energy loss.

Construction of efficient electrochemiluminescence resonance energy transfer sensor based on SnO/SnSQDs-Ru@IRMOF-3 composite for sensitive detection of procalcitonin.

An efficient electrochemiluminescence resonance energy transfer (ECL-RET) method is proposed which combines the luminescent materials of tris(4,4'-dicarboxylicacid-2,2'-bipyridyl) ruthenium(II) (energy donor) and tin dioxide and tin disulfide quantum dots (SnO/SnSQDs) (energy acceptor) into the isoreticular metal - organic framework-3 (IRMOF-3) material to form a composite. In this mode, the distance between the energy donor and the acceptor was greatly shortened, reducing the energy loss, and thereby effectively improving RET efficiency and further significantly improving the ECL signal. The obtained composite (SnO/SnSQDs-Ru@IRMOF-3) was combined with sandwich immunoreaction to construct an ECL immunosensor for the sensitive detection of procalcitonin (PCT).

Electrochemiluminescence resonance energy transfer of MnCO for ultrasensitive amyloid-β protein detection.

A composite material MnCO/poly(diallyl dimethyl ammonium chloride) (PDDA)/Ag with excellent electrochemiluminescence (ECL) performance and high biocompatibility was prepared by adding MnCO and PDDA to silver nanoparticles (AgNPs). MnCO/PDDA/Ag and Au@SiONPs were used as ECL donors and acceptors, respectively. Thus, an effective ECL-resonance energy transfer (RET) sensing platform was established.

An electrochemiluminescence immunosensor based on ABEI-GO-AgNPs as a double-amplified luminophore for the ultra-sensitive detection of prostate-specific antigen.

A sandwich electrochemiluminescence (ECL) immunosensor based on an N-(4-aminobutyl)-N-ethylisoluminol-graphene oxide-Ag nanoparticle (ABEI-GO-AgNPs) complex and cysteine silver nanowires (AgCysNWs) was prepared to detect prostate-specific antigen (PSA). Our results showed that an ECL signal probe, ABEI-GO-AgNPs, with an ultrahigh specific surface area, favorable catalytic properties, and electrical conductivity was prepared by a one-step synthesis method. ABEI-GO-AgNPs with good biocompatibility immobilized secondary antibody (Ab) via AgN bonds.

Carbon quantum dots with blue/near infrared emissions for ratiometric fluorescent lornoxicam sensing and bio-imaging.

An economical and eco-friendly hydrothermal method for the preparation of nitrogen-doped carbon quantum dots (N-CQDs) was studied with rambutan peel and lysine. The morphology, structure, and optical properties of N-CQDs were characterized by transmission electron microscopy, Fourier transform infrared spectrometry, X-ray powder diffractometer, X-ray photoelectron spectrometry, and UV spectrophotometry. The synthesized N-CQDs have excellent characteristics such as strong fluorescence, good dispersion, high stability, and excellent water solubility.

Spatially-resolved dual-potential sandwich electrochemiluminescence immunosensor for the simultaneous determination of carbohydrate antigen 19-9 and carbohydrate antigen 24-2.

A new electrochemiluminescence (ECL) immunosensor based on spatially-resolved dual-potential technology was designed for the simultaneous determination of carbohydrate antigen 19-9 (CA 19-9) and carbohydrate antigen 24-2 (CA 242). Luminol-AgNPs@ZIF-67 was used as the anodic probe, and Pt nanoparticle-functionalized graphitic carbon nitride nanosheets (g-CN@PtNPs) were used as the cathodic probe. Two spatially-resolved areas on the dual-disk glassy carbon electrode (DDGCE) were modified with a AuNPs film by electrodeposition to improve the conductivity of the sensing interface.

A potential-resolved electrochemiluminescence resonance energy transfer strategy for the simultaneous detection of neuron-specific enolase and the cytokeratin 19 fragment.

An electrochemiluminescence resonance energy transfer (ECL-RET) immunosensor was developed based on the potential-resolved technology for the simultaneous detection of neuron-specific enolase (NSE) and the cytokeratin 19 fragment (CYFRA21-1). The absorption spectrum of gold nanorods (AuNRs) perfectly overlapped with the ECL spectra of SnS2@Pt and Ru(bpy)32+/Zn-MOF, so they exhibited an excellent ECL-RET effect with high efficiency. Zn-MOF possesses a large surface area, which allows for the loading of Ru(bpy)32+.

N-doped carbon quantum dots from osmanthus fragrans as a novel off-on fluorescent nanosensor for highly sensitive detection of quercetin and aluminium ion, and cell imaging.

In this work, fluorescent N-doped carbon quantum dots (N-CQDs) have been synthesized by simple hydrothermal heating of natural osmanthus fragrans, without any toxic ingredients or surface chemical modifications. The N-CQDs possess a high quantum yield of 21.9 %, outstanding blue fluorescence, good water dispersity, and excellent optical stability.

Electrochemiluminescence immunoassay of human chorionic gonadotropin using silver carbon quantum dots and functionalized polymer nanospheres.

A composite, reduced graphene oxide (rGO) doped with silver nanoparticles (Ag NPs), was prepared by using binary reductants of sodium citrate and hydrazine hydrate. Carbon quantum dots (CQDs) synthesized by papaya peel combined with silver ions to form a CQDs-loaded silver nanoparticle (AgCQDs) nanocomposite. Polymer nanospheres (PNS) were generated via the infinite coordination polymer of ferrocene dicarboxylic acid and employed as carriers to load AgCQDs.

Electrochemiluminescent immunoassay for neuron specific enolase by using amino-modified reduced graphene oxide loaded with N-doped carbon quantum dots.

An ultrasensitive electrochemiluminescence based sandwich immunoassay is presented for determination of neuron specific enolase. The method uses silver-cysteine nanowires as the capture probe and a composite made of amino-modified reduced graphene oxide and nitrogen-doped carbon quantum dots as the signal probe. It was synthesized by covalent coupling of amino-modified reduced graphene oxide to the carboxy groups of nitrogen-doped carbon quantum dots.

Facile Preparation of Boron and Nitrogen Codoped Green Emission Carbon Quantum Dots for Detection of Permanganate and Captopril.

A hydrothermal strategy for preparing boron and nitrogen codoped carbon quantum dots was studied using the precursors of -amino salicylic acid, boric acid and ethylene glycol dimethacrylate. The boron and nitrogen codoped carbon quantum dots have high fluorescence intensity, good monodispersity, high stability, superior water solubility, and a fluorescence quantum yield of 19.6%.

A Novel Carbon Quantum Dots Signal Amplification Strategy Coupled with Sandwich Electrochemiluminescence Immunosensor for the Detection of CA15-3 in Human Serum.

A sensitive sandwich electrochemiluminescence immunosensor was established by employing graphene oxide-PEI-carbon quantum dots (CQDs)-Au nanohybrid as probe to measure carbohydrate antigen 15-3 (CA15-3), a breast cancer biomarker. In this work, nanocomposites of Ag nanoparticles and polydopamine (AgNPs-PDA) were synthesized by redox reaction between dopamine and Ag. The nanocomposite with high surface area can provide an efficient substrate for immobilizing initial antibody (Ab1).

Ginkgo leaf-based synthesis of nitrogen-doped carbon quantum dots for highly sensitive detection of salazosulfapyridine in mouse plasma.

A simple, economical hydrothermal strategy for synthesizing nitrogen-doped carbon quantum dots (N-CQDs) was developed using Ginko leaves as a carbon source. These N-CQDs have strong blue fluorescence, excitation-relevant emissions, high monodispersity, good stability, good water solubility, and a 22.8% fluorescence quantum yield.