Correlation analysis of human papillomavirus E6/E7 mRNA detection with diagnosis, prognosis and recurrence risk in patients with cervical epithelioma.

Background: Cervical intraepithelial neoplasia (CIN) is an important precursor of cervical cancer. Early detection and treatment can reduce the incidence of cervical cancer.

Aim: To investigate the detection rate of human papillomavirus (HPV) E6/E7 mRNA in cervical tissue of patients with different types of epithelial cell neoplasia (CIN) and its relationship with CIN progression and diagnosis.

Knowledge, attitude and practice of contraceptive methods among women with an unplanned pregnancy.

Objectives: The study aimed to investigate the knowledge, attitude and practice (KAP) of contraceptive methods among women with an unplanned pregnancy, aiming to improve their reproductive health and increase their understanding of contraceptive methods.

Design: This is a cross-sectional study.

Setting: The study was conducted at the Maternity and Child Healthcare Hospital of Hubei between 20 November 2022 and 20 January 2023.

Photocatalytic Degradation of Malachite Green by Titanium Dioxide/Covalent Organic Framework Composite: Characterization, Performance and Mechanism.

In this paper, a titanium dioxide/covalent organic framework (TiO/COF) composite was prepared and its photocatalytic removal of dye was investigated. Using tetrabutyl titanate as a titanium source, TiO nanomaterial was prepared by sol-gel method. In the presence of TiO, TiO/COF core-shell composite was prepared by solvothermal synthesis using melamine and 1,4-phthalaldehyde as ligands.

Improving Interfacial Adhesion of Graphite/Epoxy Composites by Surface Functionalization.

Graphite/epoxy resin (G/EP) composites are extensively utilized in bipolar plates for fuel cells owing to their outstanding electrical and mechanical properties. However, the mechanical strength of these composites declines notably due to the inadequate bonding interface between graphite and epoxy resin. To address this issue, we used molecular dynamics (MD) simulations to study the influence of graphite surface functionalization on the interfacial structures of composites.

Real-world outcomes of PD-L1 inhibitors combined with thoracic radiotherapy in the first-line treatment of extensive stage small cell lung cancer.

Background: The CREST study showed that the addition of thoracic radiotherapy (TRT) could improve the survival rate in patients with extensive stage small cell lung cancer (ES-SCLC), but whether TRT can bring survival benefit in the era of immunotherapy remains controversial. This study aimed to explore the efficacy and safety of adding TRT to the combination of PD-L1 inhibitors and chemotherapy.

Methods: The patients who received durvalumab or atezolizumab combined with chemotherapy as the first-line treatment of ES-SCLC from January 2019 to December 2021 were enrolled.

Integrated multiple microarray studies by robust rank aggregation to identify immune-associated biomarkers in Crohn's disease based on three machine learning methods.

Crohn's disease (CD) is a complex autoimmune disorder presumed to be driven by complex interactions of genetic, immune, microbial and even environmental factors. Intrinsic molecular mechanisms in CD, however, remain poorly understood. The identification of novel biomarkers in CD cases based on larger samples through machine learning approaches may inform the diagnosis and treatment of diseases.

Serum Spondin-2 expression, tumor invasion, and antitumor immune response in patients with cervical cancer.

Background: Cervical cancer is a gynecological malignancy common in middle-aged and older patients, with a high mortality rate. Spondin-2 is an extracellular matrix protein that involved in innate and acquired immune responses. Herein, we investigated the relationship between serum Spondin-2 expression, tumor invasion and infiltration, and immune response in patients with cervical cancer and provided a theoretical basis for clinical practice.

Establishment and validation of a risk prediction model for high-grade cervical lesions.

Objective: To establish and validate a risk prediction model for cervical high-grade squamous intraepithelial lesions (HSIL).

Methods: This retrospective study included patients who underwent cervical biopsies at the Cervical Disease Centre of Maternal and Child Hospital of Hubei Province between January 2021 and December 2021.

Results: A total of 1630 patients were divided into the HSIL + cervical lesion group (n = 186) and the ≤ LSIL cervical lesions group (n = 1444).

A short peptide encoded by long non-coding RNA small nucleolar RNA host gene 6 promotes cell migration and epithelial-mesenchymal transition by activating transforming growth factor-beta/SMAD signaling pathway in human endometrial cells.

Background: Endometrial dysfunction is closely correlated with the development of multiple severe gynecological disorders including intrauterine adhesion. Accumulating evidence supports that some long non-coding RNAs (lncRNAs) have peptide-coding potential. In this text, the peptide-coding ability of lncRNA SNHG6 was examined.

Reduced Adenoma Miss Rate With 9-Minute vs 6-Minute Withdrawal Times for Screening Colonoscopy: A Multicenter Randomized Tandem Trial.

Introduction: Although the 9-minute mean withdrawal time (m-WT) is often reported to be associated with the optimal adenoma detection rate (ADR), no randomized trials of screening colonoscopy have confirmed the impact of a 9-minute m-WT on adenoma miss rate (AMR) and ADR.

Methods: A multicenter tandem trial was conducted in 11 centers. Seven hundred thirty-three asymptomatic participants were randomized to receive segmental tandem screening colonoscopy with a 9-minute withdrawal, followed by a 6-minute withdrawal (9-minute-first group, 9MF, n = 366) or vice versa (6-minute-first group, 6MF, n = 367).

Determination of Pb by Colorimetric Method Based on Catalytic Amplification of Ag Nanoparticles Supported by Covalent Organic Frameworks.

In this paper, covalent organic frameworks (COFs) are prepared by solvothermal synthesis using 1,3,5-benzenetricarboxaldehyde and benzidine as ligands. Then, using COFs as a template, AgCOFs with high catalytic activity is prepared by in situ loading silver nanoparticles (AgNC) on the surface of COFs by sodium borohydride reduction method. AgCOFs are characterized by TEM, SEM, FTIR and XRD.

A comparative study on roles of natural killer T cells in two diet-induced non-alcoholic steatohepatitis-related fibrosis in mice.

Background: Immune responses are important in the progression of non-alcoholic fatty liver disease (NAFLD). Natural killer T (NKT) cells are main components of the innate immune system that modulate immunity. However, the role of NKT cells in NAFLD remains controversial.

SPI1-related protein inhibits cervical cancer cell progression and prevents macrophage cell migration.

Aim: The functions and molecular mechanisms of SPI1-related protein (SPIB) were examined in cervical cancer (CC) cells.

Methods: Genes related to miscarriage and prognosis in CC were identified by Kaplan-Meier and differential expression analysis, respectively. Cell proliferation, apoptosis, migration, and invasion were examined by cell counting kit-8, flow cytometry, transwell migration, and transwell invasion assays, respectively.

The novel circ_0084904/miR-802/MAL2 axis promotes the development of cervical cancer.

Circular RNAs (circRNAs) have been identified as critical regulators in human cancers, including cervical cancer (CC). However, the precise action of circ_0084904 in cervical carcinogenesis remains to be elucidated. The levels of circ_0084904, microRNA (miR)-802, and Mal, T cell differentiation protein 2 (MAL2) were checked by quantitative real-time PCR (qRT-PCR) or western blot.

Diagnostic value of transvaginal three-dimensional ultrasound combined with color Doppler ultrasound for early cesarean scar pregnancy.

Background: Our study sought to obtain data which assess the diagnostic value of transvaginal three-dimensional ultrasound (3D-US) combined with color Doppler ultrasound (US) for early cesarean scar pregnancy (CSP).

Methods: All participants were randomly divided into a Control group diagnosed using 3D-US and a Combination group diagnosed using 3D-US combined with color Doppler US. The preoperative US results were compared with postoperative pathological results.

Identification of a Ferroptosis-Related Signature Model Including mRNAs and lncRNAs for Predicting Prognosis and Immune Activity in Hepatocellular Carcinoma.

Background: Ferroptosis is a novel form of regulated cell death involved in tumor progression. The role of ferroptosis-related lncRNAs in hepatocellular carcinoma (HCC) remains unclear.

Methods: RNA-seq and clinical data for HCC patients were downloaded from The Cancer Genome Atlas (TCGA) Genomic Data Commons (GDC) portal.

On-signal amplification of silver nanosol RRS/SERS aptamer detection of ultratrace urea by polystyrene nanosphere catalyst.

The catalytic amplification signal of polystyrene nanosphere (PN) is used to conveniently fabricate the resonance Rayleigh scattering (RRS)/surface-enhanced Raman scattering (SERS) dual-mode method to sensitively and selectively detect urea in food. PN has strong catalysis of the slow nanoreaction of citrate-Ag(I) to produce yellow silver nanoparticles (AgNP), which exhibit strong RRS effect and SERS effect with molecular probes. When aptamer (Apt) is present, the Apt is adsorbed on the PN surface, the catalysis is weakened, the AgNP is reduced, and the SERS/RRS signal is weakened.

LncRNA PVT1 promotes cervical cancer progression by sponging miR-503 to upregulate ARL2 expression.

Cervical cancer (CC) is a huge threat to the health of women worldwide. Long non-coding RNA plasmacytoma variant translocation 1 gene (PVT1) was proved to be associated with the development of diverse human cancers, including CC. Nevertheless, the exact mechanism of PVT1 in CC progression remains unclear.

Coronavirus Disease 2019 (COVID-19): Prevention and Control in Gynecological Outpatient Clinic.

The pandemic of coronavirus disease 2019 (COVID-19) has become a major public health challenge around the world, and outbreaks of the SARS-CoV-2 have constituted a public health emergency of international concern. Infection control measures are necessary to prevent further spread of the virus and to help control the epidemic situation. Due to the characteristics of gynecological settings, the risk of cross infection between patients and gynecologic practitioners can be high, strict and effective infection control protocols are urgently needed.

A Highly Sensitive SERS and RRS Coupled Di-Mode Method for CO Detection Using Nanogolds as Catalysts and Bifunctional Probes.

Carbon monoxide (CO) is a commonly poisonous gas. It is important to detect CO in daily life. Herein, a new and sensitive surface enhanced Raman scattering (SERS) and resonance Rayleigh scattering (RRS) coupled di-mode method was developed for CO, based on gold nano-enzyme catalysis and gold nanoprobes.

New Ag-Doped COF Catalytic Amplification Aptamer Analytical Platform for Trace Small Molecules with the Resonance Rayleigh Scattering Technique.

Covalent organic frameworks (COFs) and Ag-doped COFs (AgCOFs) are prepared by the polycondensation procedure and characterized by electron microscopy and molecular spectral techniques. Their catalysis of the CuO particle reaction of glucose (GL)-Cu(II) was examined by resonance Rayleigh scattering (RRS), and AgCOFs were found to exhibit the strongest catalysis. The melamine (ML) aptamers (Apt) can attach to the surface of AgCOF and inhibit its catalytic activity.

A highly sensitive and accurate SERS/RRS dual-spectroscopic immunosensor for clenbuterol based on nitrogen/silver-codoped carbon dots catalytic amplification.

Preparation of multifunctional codoped carbon dots, and their new analytical applications in surface-enhanced Raman scattering (SERS) quantitative analytical method are still challenge. To overcome these problems, the nitrogen/silver-codoped carbon dots (CD) with highly catalytic amplification are prepared by microwave method, and characterized by spectrophotometry and electron microscopy. The results show that CD can strongly catalyze trisodium citrate-HAuCl reaction to generate red nanogold with resonance Rayleigh scattering (RRS) effect and SERS effect using Victoria blue B (VBB) as molecular probes.

A Novel Fluorescence Resonance Energy Transfer-Based High-Throughput Screening Method for Generation of Lysozyme with Improved Antimicrobial Activity against Strains.

Lysozyme has emerged to be a promising alternative to traditional antibiotics to deal with the increasing antibiotic resistance of bacteria. However, its application is hampered by its inferior bactericidal activity against Gram-negative bacteria. To address this problem, a novel "enzyme-cascade fluorescent high-throughput screening (HTS) method" was designed and constructed based on detection of fluorescence resonance energy transfer (FRET) and enzyme-cascade reaction of lysozyme and protease.

A fluorometric clenbuterol immunoassay using sulfur and nitrogen doped carbon quantum dots.

A fluorometric clenbuterol immunoassay is described that uses S- and N-co-doped carbon quantum dots as the fluorescent probe. Strongly fluorescent S/N-doped carbon quantum dots (S/N-CDs) were synthesized by hydrothermal method using fructose as the carbon precursor and L-cysteine as S/N sources. The S/N-CDs were characterized by transmission electron microscopy, energy dispersive spectroscopy and Fourier transform infrared spectroscopy (FTIR).