[Identification of a novel KIR3DL3*064 allele by cDNA cloning and sequencing].

Objective: To report on a novel KIR3DL3 allele identified in a southern Han Chinese individual.

Methods: Peripheral blood sample was collected from a voluntary blood donor with inconclusive result by KIR3DL3 sequence-based typing (SBT). Total mRNA was extracted and subjected to reverse transcription to obtain KIR3DL3 cDNA, which was then amplified by PCR with a pair of KIR3DL3-specific primers.

Full-length sequence of a novel allele, HLA-B*40:01:45, identified in a patient with hepatitis B infection.

HLA-B*40:01:45 differs from HLA-B*40:01:02 by a single nucleotide change in exon 1, 33 G > A (codon -14 CTG > CTA).

[Establishment of a quality control system for HLA allele typing and its key points].

Objective: To list the key points for quality control during HLA-A, B, C, DRB1 and DQB1 allele typing by taking consideration of hardware, software and experimental procedures.

Methods: A total of 10 167 samples from randomly selected healthy blood donors and donor-recipient pairs from Shenzhen were typed for exons 2-4 of HLA-A, B, C, exon 2 of HLA-DRB1, and exons 2 and 3 of HLA-DQB1 by PCR- sequence-based typing. For 56 cases whose forward and reverse sequences were inconsistent, the samples were re-checked by a PCR-sequence specific oligonucleotide probe method.

Residual risk of transfusion-transmitted viral infections in Shenzhen, China, 2001 through 2004.

Background: There are no current estimates of the residual risks of transmission by blood of hepatitis B virus (HBV) or hepatitis C virus (HCV) and human immunodeficiency virus (HIV) in China. Such estimates are an essential prerequisite to monitoring and improving transfusion safety as well as supporting evidence based assessment of the value of implementing new screening interventions.

Study Design And Methods: Viral screening data for donors from Shenzhen, China, for the period 2001 to 2004, were retrospectively analyzed.