Single-cell genetic models to evaluate orphan gene function: The case of QQS regulating carbon and nitrogen allocation.

We demonstrate two synthetic single-cell systems that can be used to better understand how the acquisition of an orphan gene can affect complex phenotypes. The Arabidopsis orphan gene, () has been identified as a regulator of carbon (C) and nitrogen (N) partitioning across multiple plant species. modulates this important biotechnological trait by replacing NF-YB (Nuclear Factor Y, subunit B) in its interaction with NF-YC.

Venous Thrombus Embolism in Polytrauma: Special Attention to Patients with Traumatic Brain Injury.

Venous thrombus embolism (VTE) is common after polytrauma, both of which are considered significant contributors to poor outcomes and mortality. Traumatic brain injury (TBI) is recognized as an independent risk factor for VTE and one of the most common components of polytraumatic injuries. Few studies have assessed the impact of TBI on the development of VTE in polytrauma patients.

Multifactorial Shock: A Neglected Situation in Polytrauma Patients.

Background: Shock after traumatic injury is likely to be hypovolemic, but different types of shock (distributive shock, obstructive shock, or cardiogenic shock) can occur in combination, known as multifactorial shock. Multifactorial shock is a neglected area of study, and is only reported sporadically. Little is known about the incidence, characteristics, and outcomes of multifactorial shock after polytrauma.

Multi-omics analysis reveals the molecular changes accompanying heavy-grazing-induced dwarfing of .

Heavy grazing significantly reduces growth. To enhance our understanding of plant responses to heavy grazing, we conducted transcriptomic, proteomic, and metabolic analyses of the leaves of non-grazed plants (NG) and heavy-grazing-induced dwarf plants (HG) of . A total of 101 metabolites, 167 proteins, and 1,268 genes differed in abundance between the HG and NG groups.

Clinical Efficacy and Drug Sensitivity Test Results of Azithromycin Combined With Other Antimicrobial Therapies in the Treatment of MDR Ventilator-Associated Pneumonia.

The aim of the research was to study the effect of azithromycin (AZM) in the treatment of MDR VAP combined with other antimicrobial therapies. The clinical outcomes were retrospectively collected and analyzed to elucidate the efficacy of different combinations involving azithromycin in the treatment of MDR-PA VAP. The minimal inhibitory concentration (MIC) of five drugs was measured by the agar dilution method against 27 isolates of MDR-PA, alone or in combination.

De novo transcriptomic profiling of the clonal Leymus chinensis response to long-term overgrazing-induced memory.

Sheepgrass (Leymus chinensis) is one of the dominant grass species present on typical steppes of the Inner Mongolia Plateau. However, L. chinensis has developed a dwarfing phenotype in response to the stressful habitat in grasslands that are severely degraded due to heavy grazing.

Identification of the WRKY gene family and functional analysis of two genes in Caragana intermedia.

Background: WRKY transcription factors, one of the largest families of transcriptional regulators in plants, play important roles in plant development and various stress responses. The WRKYs of Caragana intermedia are still not well characterized, although many WRKYs have been identified in various plant species.

Results: We identified 53 CiWRKY genes from C.

Calmodulin-binding protein CBP60g functions as a negative regulator in Arabidopsis anthocyanin accumulation.

Anthocyanins, a kind of flavonoid, normally accumulate in the flowers and fruits and make them colorful. Anthocyanin accumulation is regulated via the different temporal and spatial expression of anthocyanin regulatory and biosynthetic genes. CBP60g, a calmodulin binding protein, has previously been shown to have a role in pathogen resistance, drought tolerance and ABA sensitivity.

Selection of Reference Genes for qRT-PCR Analysis of Gene Expression in Stipa grandis during Environmental Stresses.

Stipa grandis P. Smirn. is a dominant plant species in the typical steppe of the Xilingole Plateau of Inner Mongolia.

De novo assembly and transcriptomic profiling of the grazing response in Stipa grandis.

Background: Stipa grandis (Poaceae) is one of the dominant species in a typical steppe of the Inner Mongolian Plateau. However, primarily due to heavy grazing, the grasslands have become seriously degraded, and S. grandis has developed a special growth-inhibition phenotype against the stressful habitat.

Methods for analysis of disease resistance and the defense response in Arabidopsis.

Many G proteins are involved in the plant defense responses against pathogen infection. With Arabidopsis as a model, this chapter describes the protocols commonly used for inoculating plants with Pseudomonas syringae (a bacterial pathogen) and Botrytis cinerea (a fungal pathogen) for analyzing disease resistance phenotypes caused by these pathogens. In addition, the procedures are provided for observation of the hypersensitive response triggered by avirulent strains of P.

Calmodulin-binding protein CBP60g is a positive regulator of both disease resistance and drought tolerance in Arabidopsis.

Unlabelled: Calmodulin-binding proteins (CBPs) have been known to be involved in both biotic and abiotic stress responses. Recently, two closely related CBPs, Arabidopsis SAR Deficient 1 and CBP60g, were found to belong to a new family of transcription factors that regulate salicylic acid (SA) biosynthesis triggered by microbe-associated molecular patterns. In this study, we found that overexpression of CBP60g in Arabidopsis caused elevated SA accumulation, increased expression of the defense genes, and enhanced resistance to Pseudomonas syringae.