Publications by authors named "Dongkun Wang"
Tobacco bacterial wilt, which is caused by Ralstonia solanacearum, is a devastating soilborne disease of tobacco worldwide and is widespread in the continuously acidic fields of southern China. Here, the fumigation activity under different pH conditions, component identification, and bioactivity of the volatile organic compounds (VOCs) produced by an acid-tolerant strain, Pseudomonas protegens CLP-6, were investigated. There was a wide antimicrobial spectrum of the VOCs against phytopathogens, including four bacteria, eight fungi, and two oomycetes.
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- Tobacco is a significant crop whose productivity is threatened by a new disease observed in Shandong Province, China, affecting the NC102 variety during crucial growth stages, leading to high infection rates.
- Symptoms start as small chlorotic spots that enlarge into irregular necrotic lesions, and research includes isolating pathogens from infected leaves through a sterilization and culturing process on nutrient agar, revealing yellow colonies.
- Genetic analysis of 30 isolates showed 100% nucleotide identity in the 16S rRNA gene, with one isolate (ZC5) being closely related to known strains of Pseudomonas psychrotolerans, confirming its identity through sequence alignment.
Article Synopsis
- Tobacco is a vital crop in southern China, where an uncharacterized disease affecting it was identified in 2020-2021, particularly in Tongren city, with incidence rates between 20%-100%.
- The disease manifested through yellow-brown necrotic spots on leaves and stems, prompting researchers to collect samples for pathogen isolation and identification.
- Laboratory analysis involved standard procedures for isolating and characterizing fungal pathogens, resulting in sequence data that matches closely with those of Nigrospora oryzae from previous studies.
Tobacco bacterial wilt is one of the most devastating soil-borne diseases in tobacco-producing regions worldwide. It is often responsible for significant economic losses during tobacco production. A rapid, specific, and high-throughput on-site detection method is important for plant disease management.
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- Bacterial wilt, caused by Ralstonia solanacearum, affects important crops, and current management strategies are limited.
- A study isolated Bacillus amyloliquefaciens strain Cas02 from healthy tobacco plants, showing significant plant growth promotion and the ability to suppress the bacterial wilt pathogen.
- Cas02 produced key growth-promoting substances and was effective in both greenhouse and field tests, with its antimicrobial properties linked to identified compounds and genetic mechanisms, highlighting its potential as a biocontrol agent.
Introduction: The prevalence of mild cognitive impairment (MCI) in the elderly population aged 60 to 84 years ranges from 6.7% to 25.2%, and the effective prevention and reversal of MCI progression to Alzheimer disease (AD) is crucial.
View Article and Find Full Text PDFDemand for antimicrobial packaging films is growing due to public attention to food safety. The structures and properties of gellan gum-guar gum blend films incorporated with nisin were investigated in this paper. Fourier transform infrared spectroscopy, rheological analyses showed intermolecular interactions among gellan gum, guar gum, and nisin.
View Article and Find Full Text PDFPurpose: To examine the benefits of different numbers of 1064-nm Nd-YAG laser treatments in patients with onychomycosis.
Methods: This was a pilot study of patients with onychomycosis who were divided into three groups: four treatment sessions (group A), eight sessions (group B), and 12 sessions (group C). Only infected nails of degrees II-III (Scoring Clinical Index for Onychomycosis) were included.
Background: Recent research shows that lasers can inhibit fungal growth and that Nd:YAG 1064-nm lasers can penetrate as deep as the lower nail plate. The aim of this study was to observe the effect of a long-pulse Nd:YAG 1064-nm laser on 154 nails of 33 patients with clinically and mycologically proven onychomycosis.
Methods: Thirty-three patients with 154 nails affected by onychomycosis were randomly assigned to two groups, with the 154 nails divided into three sub-groups (II degree, III degree, and IV degree) according to the Scoring Clinical Index of Onychomycosis.