Enhancing the photo-induced oxidase-like activity of fluorescein with methyl viologen for colorimetric detection of organophosphorus pesticide.

Overcoming the intrinsic low activity of most photoinduced oxidase mimics has been extremely challenging. In this work, we developed a methyl viologen (MV) mediated strategy to enhance the oxidase-like activity of fluorescein. The presence of MV gives it a high affinity for TMB with a low Michaelis-Menten constant (K) of 0.

Comparison between liraglutide alone and liraglutide in combination with insulin on osteoporotic rats and their effect on bone mineral density.

Objectives: To compare the therapeutic efficacy of liraglutide (LRG) single drug combined with insulin (Ins) on osteoporosis in rats and its effect on bone mineral density (BMD). A rat model of diabetes combined with osteoporosis was established.

Methods: 40 Sprague-Dawley rats were divided into four groups (blank, control, LRG and LRG+Ins).

Enhancing the peroxidase-like activity of ficin by rational blocking thiol groups for colorimetric detection of biothiols.

The peroxidase-like activity of ficin is relatively low, which limits its application. It was found that thiol groups of ficin could inhibit its peroxidase-like activity. So, two procedures, i.

Tuning the fluorescence performance of carbon dots with a reduction pathway.

A reduction pathway was developed to tune the fluorescence quantum yield (QY) and emission wavelength of carbon dots (CDs). Reducing the original CDs (QY ca. 1.

Efficacy of hyaluronic acid after knee arthroscopy: A systematic review and meta-analysis.

Objective: To investigate the effect of hyaluronic acid on functional recovery and pain control in patients following knee arthroscopy.

Design: A systematic review and meta-analysis was conducted to explore the efficacy of hyaluronic acid following knee arthroscopy.

Subjects And Methods: Randomized controlled trials (RCTs) assessing the effect of hyaluronic acid in knee arthroscopy were included.

Hollow carbon dots labeled with FITC or TRITC for use in fluorescent cellular imaging.

Hollow carbon dots (HCDs) were prepared by a solvothermal method and conjugated to either tetramethyl rhodamine isothiocyanate (TRITC) or fluorescein-5-isothiocyanate (FITC). This resulted in HCDs with bright red or green fluorescence, with excitation/emission peaks at 550/580 and 491/520 nm, respectively. The nanocomposites are well water-soluble, remarkably photostable and biocompatible.

Intrinsic Peroxidase-like Activity of Ficin.

Ficin is classified as a sulfhydryl protease isolated from the latex of fig trees. In most cases, a particular enzyme fits a few types of substrate and catalyzes one type of reaction. In this investigation, we found sufficient proofs for the intrinsic peroxidase-like activity of ficin and designed experiments to examine its effectiveness in a variety of scenarios.

Detection of glucose based on the peroxidase-like activity of reduced state carbon dots.

It was found that reduced state carbon dots (r-CDs) possessed intrinsic peroxidase-like activity, and could catalytically oxidize 3,3',5,5'-tetramethylbenzidine (TMB) by H2O2 to produce a color reaction. The effects of temperature, pH, incubating time and the concentration of H2O2 and TMB on catalytic activity of r-CDs were investigated. Finally we calculated the kinetic constant was ca.