Extreme rainfall trends of 21 typical urban areas in China during 1998-2015 based on remotely sensed data sets.

With the increase of population, many cities are growing in size at a phenomenal rate. Urbanization changes the urban underlying surface, influences the micro-climate, and sometimes affects the local precipitation process. In this study, we investigated the trends of extreme rainfall in China's 21 typical urban areas.

[Expression of HOXC4 gene during early development in zebrafish].

Objective: To investigate the mechanism of HOXC4 gene during early development in zebrafish, study it's expression in hematopoiesis system with whole mount in situ hybridization (WISH), then make a foundation work for further study of HOXC4 gene in hematopoiesis development.

Methods: The total RNA of different phases of zebrafish embryos was extracted, and a fragment of HOXC4 gene was cloned with RT-PCR, then HOXC4 gene fragment and pCS2 was digested with BamH I and Xho I restriction enzyme, after that HOXC4 gene fragment was inserted into pCS2+ vector. After confirming the constructed plasmid, the digoxingenin-labeled anti-sence mRNA probe of HOXC4 gene was synthesized in vitro by using T3 RNA polymerase, the exprression pattern of HOXC4 during zebrafish embryogenesis using anti-sence probe with WISH was conducted.

[Expression pattern of hoxd3 gene during early development of wild-type zebrafish embryos].

Objective: To investigate the expression pattern of hoxd3 gene during early embryogenesis and angiogenesis of wild-type zebrafish.

Methods: Total RNA was extracted from embryos of zebrafish in different development stages by trizol. The cDNA of hoxd3 gene was amplified by RT-PCR.