Implications of ubiquitination and the maintenance of replication fork stability in cancer therapy.

DNA replication forks are subject to intricate surveillance and strict regulation by sophisticated cellular machinery. Such close regulation is necessary to ensure the accurate duplication of genetic information and to tackle the diverse endogenous and exogenous stresses that impede this process. Stalled replication forks are vulnerable to collapse, which is a major cause of genomic instability and carcinogenesis.

Determination of trace potassium permanganate in tap water by solid phase extraction combined with spectrophotometry.

A simple solid phase extraction (SPE) coupled with spectrophotometric method was developed for determination of trace permanganate (MnO ) in water. Commercial reagent polyamined-6 powder was used as SPE stationary phase to retain MnO which is based on the fact that MnO can be adsorbed on polyamide 6 (PA-6) sorbent as water samples flow through the SPE cartridge. 3,3',5,5'-tetramethylbenzidine (TMB) were used both as eluent solution and chromogenic agent to convert the adsorbed MnO into Mn and generate blue oxidized product with high molar absorptivity.

Oxidative cleavage of cellulose in the horse gut.

Background: Lytic polysaccharide monooxygenases (LPMOs) belonging to the auxiliary activity 9 family (AA9) are widely found in aerobic fungi. These enzymes are O-dependent copper oxidoreductases that catalyze the oxidative cleavage of cellulose. However, studies that have investigated AA9 LPMOs of aerobic fungi in the herbivore gut are scare.

A new type of zinc ion hybrid supercapacitor based on 2D materials.

Zinc ion hybrid supercapacitors (ZICs) are truly promising competitors in prospective extensive electrochemical energy storage fields due to their cost-effectiveness, environmentally friendly nature, inherent security, and satisfying gravimetric energy density. Thus, several investigation endeavors have been dedicated to the construction and exploitation of high-performance ZICs. However, the exploitation of ZICs is still in its preliminary stage and there are many problems that need to be overcome before their potential can be fully realized.

A broad range frequency measurement method for continuous and pulsed THz waves.

This paper proposes a method to measure the frequency of terahertz (THz) waves based on the Zeeman effect and the high magnetic field technology with a wideband range from 60 GHz to 3 THz. As the frequency of THz waves absorbed by the sample is linear to the magnetic field in the Zeeman effect, the frequency can be measured by the magnetic field strength. A comparison study of THz frequency measurement was carried out in two magnet systems (a superconducting one and a pulsed one) to investigate the performance in two kinds of high magnetic fields.

Chinese Herbal Medicine Effects on Muscle Atrophy Induced by Simulated Microgravity.

Background: Skeletal muscle atrophy is a striking example of the multiple changes in the physiological state of humans and animals induced by microgravity. Previous studies have shown that a blood circulation disorder may be a cause of this atrophy, and traditional Chinese medicine has been regarded as a potential countermeasure to reverse the atrophy in China. This study was carried out to test the effects of Xuefuzhuyu capsules (XFZY) on the skeletal muscle atrophy induced by simulated microgravity.

Notch2 is required for inflammatory cytokine-driven goblet cell metaplasia in the lung.

The balance and distribution of epithelial cell types is required to maintain tissue homeostasis. A hallmark of airway diseases is epithelial remodeling, leading to increased goblet cell numbers and an overproduction of mucus. In the conducting airway, basal cells act as progenitors for both secretory and ciliated cells.

Function and distribution of circulating human PCSK9 expressed extrahepatically in transgenic mice.

Proprotein convertase subtilisin/kexin type 9 (PCSK9) is predominantly expressed in liver and regulates cholesterol metabolism by down regulating liver LDL receptor (LDLR) proteins. Here we report transgenic overexpression of human PCSK9 in kidney increased plasma levels of PCSK9 and subsequently led to a dramatic reduction in liver LDLR proteins. The regulation of LDLR by PCSK9 displayed tissue specificity, with liver being the most responsive tissue.

Pharmacologic inhibition of site 1 protease activity inhibits sterol regulatory element-binding protein processing and reduces lipogenic enzyme gene expression and lipid synthesis in cultured cells and experimental animals.

Sterol regulatory element-binding proteins (SREBPs) are major transcriptional regulators of cholesterol, fatty acid, and glucose metabolism. Genetic disruption of SREBP activity reduces plasma and liver levels of cholesterol and triglycerides and insulin-stimulated lipogenesis, suggesting that SREBP is a viable target for pharmacological intervention. The proprotein convertase SREBP site 1 protease (S1P) is an important posttranscriptional regulator of SREBP activation.

Structural and biophysical studies of PCSK9 and its mutants linked to familial hypercholesterolemia.

Proprotein convertase subtilisin kexin type 9 (PCSK9) lowers the abundance of surface low-density lipoprotein (LDL) receptor through an undefined mechanism. The structure of human PCSK9 shows the subtilisin-like catalytic site blocked by the prodomain in a noncovalent complex and inaccessible to exogenous ligands, and that the C-terminal domain has a novel fold. Biosensor studies show that PCSK9 binds the extracellular domain of LDL receptor with K(d) = 170 nM at the neutral pH of plasma, but with a K(d) as low as 1 nM at the acidic pH of endosomes.