Generation of Inheritable A-to-G Transitions Using Adenine Base Editing and NG-PAM Cas9 in .

Towards precise genome editing, base editors have been developed by fusing catalytically compromised Cas9 with deaminase components, mediating C-to-T (cytosine base editors) or A-to-G (adenine base editors) transition. We developed a set of vectors consisting of a 5'-NG-3' PAM-recognizing variant of SpCas9 with adenosine deaminases TadA7.10 or TadA8e.

Application of high-polarity hydroxyl polyacrylate pressure sensitive adhesive in rizatriptan transdermal drug delivery patch.

This study aimed to design a rizatriptan (RIZ) transdermal patch by combining of high-polarity hydroxyl pressure sensitive adhesive (PSA) AAOH-45 with an ion-pair strategy and investigate the molecular mechanism of high content hydroxyl PSA to enhance drug-PSA miscibility. RIZ free base, ion-pair complexes and PSAs containing hydroxyl group were prepared and characterized. Formulation factors including counter-ions, PSAs, drug-loading and others were optimized through single-factor studies and evaluated through pharmacokinetic studies and skin irritation tests.

Assessment of urban flood resilience based on the socio-ecological composite index model: a case study in Wuhua District, Kunming City, China.

Global climate change and rapid urbanization have increased the frequency of flooding, making urban flood resilience a critical objective. This article introduces a methodology for assessing urban flood resilience, utilizing a social-ecological synthesis index that integrates geographical and temporal data with Geographic Information System (GIS). The study focuses on ten administrative subdistricts in Wuhua District, Kunming City, China, and selects 18 social-ecological indicators.

Effect of intensive care unit diary on quality of life of intensive care unit survivors and their relatives: A systematic review and meta-analysis.

Aim: To evaluate the current literature on the effect of ICU diaries on the quality of life of ICU survivors and their relatives.

Design: Systematic review and meta-analysis.

Methods: We searched the online databases Web of Science, PubMed, Embase, The Cochrane Library, CNKI and WanFang from inception to April 2021.

A pyrenoid-localized protein SAGA1 is necessary for Ca-binding protein CAS-dependent expression of nuclear genes encoding inorganic carbon transporters in Chlamydomonas reinhardtii.

Microalgae induce a CO-concentrating mechanism (CCM) to maintain photosynthetic affinity for dissolved inorganic carbon (Ci) under CO-limiting conditions. In the model alga Chlamydomonas reinhardtii, the pyrenoid-localized Ca-binding protein CAS is required to express genes encoding the Ci-transporters, high-light activated 3 (HLA3), and low-CO-inducible protein A (LCIA). To identify new factors related to the regulation or components of the CCM, we isolated CO-requiring mutants KO-60 and KO-62.

Unambiguous Imaging of Static Scenes and Moving Targets with the First Chinese Dual-Channel Spaceborne SAR Sensor.

Multichannel synthetic aperture radar (SAR) is a breakthrough given the inherent limitation between high-resolution and wide-swath (HRWS) faced with conventional SAR. This paper aims to obtain unambiguous imaging of static scenes and moving targets with the first Chinese dual-channel spaceborne SAR sensor. We propose an integrated imaging scheme with the dual-channel echoes.

Essential oil of Artemisia vestita exhibits potent in vitro and in vivo antibacterial activity: Investigation of the effect of oil on biofilm formation, leakage of potassium ions and survival curve measurement.

The aim of the present study was to investigate the chemical composition of the essential oil of Artemisia vestita and to determine the antibacterial activity of the essential oil and its two major components, grandisol and 1,8‑cineole, against certain respiratory infection‑causing bacterial strains, in vitro and in vivo. The chemical composition of the essential oil was analyzed using gas chromatography‑mass spectrometry. A micro‑well dilution method was used to determine the minimum inhibition concentration (MIC) values of the essential oil and its major constituents.

Umbelliferone exhibits anticancer activity via the induction of apoptosis and cell cycle arrest in HepG2 hepatocellular carcinoma cells.

Hepatocellular carcinoma (HCC) is a highly malignant tumor, associated with poor patient prognoses, and high rates of morbidity and mortality. To date, the therapeutic strategies available for the treatment of HCC remain limited. The present study aimed to elucidate the anticancer activity of umbelliferone, a naturally occurring coumarin derivative isolated from Ferula communis, against the HepG2 HCC cell line.

Antibacterial activity and mode of action of the Artemisia capillaris essential oil and its constituents against respiratory tract infection-causing pathogens.

Inhalation therapy using essential oils has been used to treat acute and chronic sinusitis and bronchitis. The aim of the present study was to determine the chemical composition of the essential oil of Artemisia capillaris, and evaluate the antibacterial effects of the essential oil and its main components, against common clinically relevant respiratory bacterial pathogens. Gas chromatography and gas chromatography‑mass spectrometry revealed the presence of 25 chemical constituents, the main constituents being: α‑pinene, β‑pinene, limonene, 1,8‑cineole, piperitone, β‑caryophyllene and capillin.